Wheat powdery mildew resistance gene Pm13 encodes a mixed lineage kinase domain-like protein
Abstract Wheat powdery mildew is one of the most destructive diseases threatening global wheat production. The wild relatives of wheat constitute rich sources of diversity for powdery mildew resistance. Here, we report the map-based cloning of the powdery mildew resistance gene Pm13 from the wild wh...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-03-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-024-46814-7 |
| _version_ | 1827310091160780800 |
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| author | Huanhuan Li Wenqiang Men Chao Ma Qianwen Liu Zhenjie Dong Xiubin Tian Chaoli Wang Cheng Liu Harsimardeep S. Gill Pengtao Ma Zhibin Zhang Bao Liu Yue Zhao Sunish K. Sehgal Wenxuan Liu |
| author_facet | Huanhuan Li Wenqiang Men Chao Ma Qianwen Liu Zhenjie Dong Xiubin Tian Chaoli Wang Cheng Liu Harsimardeep S. Gill Pengtao Ma Zhibin Zhang Bao Liu Yue Zhao Sunish K. Sehgal Wenxuan Liu |
| author_sort | Huanhuan Li |
| collection | DOAJ |
| description | Abstract Wheat powdery mildew is one of the most destructive diseases threatening global wheat production. The wild relatives of wheat constitute rich sources of diversity for powdery mildew resistance. Here, we report the map-based cloning of the powdery mildew resistance gene Pm13 from the wild wheat species Aegilops longissima. Pm13 encodes a mixed lineage kinase domain-like (MLKL) protein that contains an N-terminal-domain of MLKL (MLKL_NTD) domain in its N-terminus and a C-terminal serine/threonine kinase (STK) domain. The resistance function of Pm13 is validated by mutagenesis, gene silencing, transgenic assay, and allelic association analyses. The development of introgression lines with significantly reduced chromosome segments of Ae. longissima encompassing Pm13 enables widespread deployment of this gene into wheat cultivars. The cloning of Pm13 may provide valuable insights into the molecular mechanisms underlying Pm13-mediated powdery mildew resistance and highlight the important roles of kinase fusion proteins (KFPs) in wheat immunity. |
| first_indexed | 2024-04-24T19:54:11Z |
| format | Article |
| id | doaj.art-bc1d4e7ee6214264beb1d998b3cf19d2 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-04-24T19:54:11Z |
| publishDate | 2024-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-bc1d4e7ee6214264beb1d998b3cf19d22024-03-24T12:26:39ZengNature PortfolioNature Communications2041-17232024-03-0115111310.1038/s41467-024-46814-7Wheat powdery mildew resistance gene Pm13 encodes a mixed lineage kinase domain-like proteinHuanhuan Li0Wenqiang Men1Chao Ma2Qianwen Liu3Zhenjie Dong4Xiubin Tian5Chaoli Wang6Cheng Liu7Harsimardeep S. Gill8Pengtao Ma9Zhibin Zhang10Bao Liu11Yue Zhao12Sunish K. Sehgal13Wenxuan Liu14The State Key Laboratory of Wheat and Maize Crop Science, College of Life Sciences, Henan Agricultural UniversityThe State Key Laboratory of Wheat and Maize Crop Science, College of Life Sciences, Henan Agricultural UniversityThe State Key Laboratory of Wheat and Maize Crop Science, College of Life Sciences, Henan Agricultural UniversityThe State Key Laboratory of Wheat and Maize Crop Science, College of Life Sciences, Henan Agricultural UniversityCollege of Agronomy, Nanjing Agricultural UniversityInstitute of Genetics and Developmental Biology, Chinese Academy of SciencesThe State Key Laboratory of Wheat and Maize Crop Science, College of Life Sciences, Henan Agricultural UniversityCrop Research Institute, Shandong Academy of Agricultural SciencesDepartment of Agronomy, Horticulture and Plant Science, South Dakota State UniversityCollege of Life Sciences, Yantai UniversityKey Laboratory of Molecular Epigenetics of the Ministry of Education (MOE), Northeast Normal UniversityKey Laboratory of Molecular Epigenetics of the Ministry of Education (MOE), Northeast Normal UniversityThe State Key Laboratory of Wheat and Maize Crop Science, College of Life Sciences, Henan Agricultural UniversityDepartment of Agronomy, Horticulture and Plant Science, South Dakota State UniversityThe State Key Laboratory of Wheat and Maize Crop Science, College of Life Sciences, Henan Agricultural UniversityAbstract Wheat powdery mildew is one of the most destructive diseases threatening global wheat production. The wild relatives of wheat constitute rich sources of diversity for powdery mildew resistance. Here, we report the map-based cloning of the powdery mildew resistance gene Pm13 from the wild wheat species Aegilops longissima. Pm13 encodes a mixed lineage kinase domain-like (MLKL) protein that contains an N-terminal-domain of MLKL (MLKL_NTD) domain in its N-terminus and a C-terminal serine/threonine kinase (STK) domain. The resistance function of Pm13 is validated by mutagenesis, gene silencing, transgenic assay, and allelic association analyses. The development of introgression lines with significantly reduced chromosome segments of Ae. longissima encompassing Pm13 enables widespread deployment of this gene into wheat cultivars. The cloning of Pm13 may provide valuable insights into the molecular mechanisms underlying Pm13-mediated powdery mildew resistance and highlight the important roles of kinase fusion proteins (KFPs) in wheat immunity.https://doi.org/10.1038/s41467-024-46814-7 |
| spellingShingle | Huanhuan Li Wenqiang Men Chao Ma Qianwen Liu Zhenjie Dong Xiubin Tian Chaoli Wang Cheng Liu Harsimardeep S. Gill Pengtao Ma Zhibin Zhang Bao Liu Yue Zhao Sunish K. Sehgal Wenxuan Liu Wheat powdery mildew resistance gene Pm13 encodes a mixed lineage kinase domain-like protein Nature Communications |
| title | Wheat powdery mildew resistance gene Pm13 encodes a mixed lineage kinase domain-like protein |
| title_full | Wheat powdery mildew resistance gene Pm13 encodes a mixed lineage kinase domain-like protein |
| title_fullStr | Wheat powdery mildew resistance gene Pm13 encodes a mixed lineage kinase domain-like protein |
| title_full_unstemmed | Wheat powdery mildew resistance gene Pm13 encodes a mixed lineage kinase domain-like protein |
| title_short | Wheat powdery mildew resistance gene Pm13 encodes a mixed lineage kinase domain-like protein |
| title_sort | wheat powdery mildew resistance gene pm13 encodes a mixed lineage kinase domain like protein |
| url | https://doi.org/10.1038/s41467-024-46814-7 |
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