Detection of deoxynivalenol (DON) using europium chelates and magnetic nanoparticles
Background: Several immunoassay methods have been developed for the detection of DON in cereal. However, there has been no method based on Eu-chelate antibody-conjugated magnetic nanoparticles established. Methods: Indirect competition for DON detection was established by combining Eu-chelate and im...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2019-01-01
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| Series: | Food and Agricultural Immunology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/09540105.2018.1548577 |
| Summary: | Background: Several immunoassay methods have been developed for the detection of DON in cereal. However, there has been no method based on Eu-chelate antibody-conjugated magnetic nanoparticles established. Methods: Indirect competition for DON detection was established by combining Eu-chelate and immunomagnetic separation. The immunocomplex was separated and washed by exposure to a magnetic field and treatment with enhancement solution; then, fluorescences were measured according to the number of europium ions dissociated. Results: The working concentrations ranged from 0.05 to 100 ng/mL, and the sensitivity was 0.5 μg/Kg in cereal. The intra–assay CVs of corn and wheat were 5.7% and 7.1%, respectively. The recovery rates obtained using cereal sample were 83.2%–120.0%. Conclusion: This study suggested that the Eu-chelate antibody-conjugated magnetic nanoparticles method was fast and sensitive in screening large quantities of samples, and could be used for detection of DON in cereal. |
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| ISSN: | 0954-0105 1465-3443 |