Detection of deoxynivalenol (DON) using europium chelates and magnetic nanoparticles
Background: Several immunoassay methods have been developed for the detection of DON in cereal. However, there has been no method based on Eu-chelate antibody-conjugated magnetic nanoparticles established. Methods: Indirect competition for DON detection was established by combining Eu-chelate and im...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2019-01-01
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| Series: | Food and Agricultural Immunology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/09540105.2018.1548577 |
| _version_ | 1818198151533690880 |
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| author | Bin Zhou Xueyu Yuan Yonghong Hu Jun Fan Wenge Yang Mingming Guo Yi Zhang Wenxin Li Jue Zhang |
| author_facet | Bin Zhou Xueyu Yuan Yonghong Hu Jun Fan Wenge Yang Mingming Guo Yi Zhang Wenxin Li Jue Zhang |
| author_sort | Bin Zhou |
| collection | DOAJ |
| description | Background: Several immunoassay methods have been developed for the detection of DON in cereal. However, there has been no method based on Eu-chelate antibody-conjugated magnetic nanoparticles established. Methods: Indirect competition for DON detection was established by combining Eu-chelate and immunomagnetic separation. The immunocomplex was separated and washed by exposure to a magnetic field and treatment with enhancement solution; then, fluorescences were measured according to the number of europium ions dissociated. Results: The working concentrations ranged from 0.05 to 100 ng/mL, and the sensitivity was 0.5 μg/Kg in cereal. The intra–assay CVs of corn and wheat were 5.7% and 7.1%, respectively. The recovery rates obtained using cereal sample were 83.2%–120.0%. Conclusion: This study suggested that the Eu-chelate antibody-conjugated magnetic nanoparticles method was fast and sensitive in screening large quantities of samples, and could be used for detection of DON in cereal. |
| first_indexed | 2024-12-12T02:01:19Z |
| format | Article |
| id | doaj.art-bc1d88a3f20047749aba298b1fcab6d4 |
| institution | Directory Open Access Journal |
| issn | 0954-0105 1465-3443 |
| language | English |
| last_indexed | 2024-12-12T02:01:19Z |
| publishDate | 2019-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Food and Agricultural Immunology |
| spelling | doaj.art-bc1d88a3f20047749aba298b1fcab6d42022-12-22T00:42:10ZengTaylor & Francis GroupFood and Agricultural Immunology0954-01051465-34432019-01-01301879410.1080/09540105.2018.15485771548577Detection of deoxynivalenol (DON) using europium chelates and magnetic nanoparticlesBin Zhou0Xueyu Yuan1Yonghong Hu2Jun Fan3Wenge Yang4Mingming Guo5Yi Zhang6Wenxin Li7Jue Zhang8Jiangsu Institute of Nuclear MedicineTongji University School of MedicineNanjing Tech UniversityJiangsu Institute of Nuclear MedicineNanjing Tech UniversityJiangsu Institute of Nuclear MedicineJiangsu Institute of Nuclear MedicineJiangsu Institute of Nuclear MedicineJiangsu Institute of Nuclear MedicineBackground: Several immunoassay methods have been developed for the detection of DON in cereal. However, there has been no method based on Eu-chelate antibody-conjugated magnetic nanoparticles established. Methods: Indirect competition for DON detection was established by combining Eu-chelate and immunomagnetic separation. The immunocomplex was separated and washed by exposure to a magnetic field and treatment with enhancement solution; then, fluorescences were measured according to the number of europium ions dissociated. Results: The working concentrations ranged from 0.05 to 100 ng/mL, and the sensitivity was 0.5 μg/Kg in cereal. The intra–assay CVs of corn and wheat were 5.7% and 7.1%, respectively. The recovery rates obtained using cereal sample were 83.2%–120.0%. Conclusion: This study suggested that the Eu-chelate antibody-conjugated magnetic nanoparticles method was fast and sensitive in screening large quantities of samples, and could be used for detection of DON in cereal.http://dx.doi.org/10.1080/09540105.2018.1548577europium chelatesmagnetic nanoparticlesdeoxynivalenol (don) |
| spellingShingle | Bin Zhou Xueyu Yuan Yonghong Hu Jun Fan Wenge Yang Mingming Guo Yi Zhang Wenxin Li Jue Zhang Detection of deoxynivalenol (DON) using europium chelates and magnetic nanoparticles Food and Agricultural Immunology europium chelates magnetic nanoparticles deoxynivalenol (don) |
| title | Detection of deoxynivalenol (DON) using europium chelates and magnetic nanoparticles |
| title_full | Detection of deoxynivalenol (DON) using europium chelates and magnetic nanoparticles |
| title_fullStr | Detection of deoxynivalenol (DON) using europium chelates and magnetic nanoparticles |
| title_full_unstemmed | Detection of deoxynivalenol (DON) using europium chelates and magnetic nanoparticles |
| title_short | Detection of deoxynivalenol (DON) using europium chelates and magnetic nanoparticles |
| title_sort | detection of deoxynivalenol don using europium chelates and magnetic nanoparticles |
| topic | europium chelates magnetic nanoparticles deoxynivalenol (don) |
| url | http://dx.doi.org/10.1080/09540105.2018.1548577 |
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