Analysis of the Effect of <i>Plutella xylostella Polycalin</i> and <i>ABCC2</i> Transporter on Cry1Ac Susceptibility by CRISPR/Cas9-Mediated Knockout
Many insects, including the <i>Plutella xylostella</i> (L.), have developed varying degrees of resistance to many insecticides, including <i>Bacillus thuringiensis</i> (<i>Bt</i>) toxins, the bioinsecticides derived from <i>Bt</i>. The polycalin protei...
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| Format: | Article |
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MDPI AG
2023-04-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/15/4/273 |
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| author | Lei Xiong Zhaoxia Liu Jingge Li Shuyuan Yao Zeyun Li Xuanhao Chen Lingling Shen Zhen Zhang Yongbin Li Qing Hou Yuhang Zhang Minsheng You Zhiguang Yuchi Shijun You |
| author_facet | Lei Xiong Zhaoxia Liu Jingge Li Shuyuan Yao Zeyun Li Xuanhao Chen Lingling Shen Zhen Zhang Yongbin Li Qing Hou Yuhang Zhang Minsheng You Zhiguang Yuchi Shijun You |
| author_sort | Lei Xiong |
| collection | DOAJ |
| description | Many insects, including the <i>Plutella xylostella</i> (L.), have developed varying degrees of resistance to many insecticides, including <i>Bacillus thuringiensis</i> (<i>Bt</i>) toxins, the bioinsecticides derived from <i>Bt</i>. The polycalin protein is one of the potential receptors for <i>Bt</i> toxins, and previous studies have confirmed that the Cry1Ac toxin can bind to the polycalin protein of <i>P. xylostella</i>, but whether polycalin is associated with the resistance of <i>Bt</i> toxins remains controversial. In this study, we compared the midgut of larvae from Cry1Ac-susceptible and -resistant strains, and found that the expression of the <i>Pxpolycalin</i> gene was largely reduced in the midgut of the resistant strains. Moreover, the spatial and temporal expression patterns of <i>Pxpolycalin</i> showed that it was mainly expressed in the larval stage and midgut tissue. However, genetic linkage experiments showed that the <i>Pxpolycalin</i> gene and its transcript level were not linked to Cry1Ac resistance, whereas both the <i>PxABCC2</i> gene and its transcript levels were linked to Cry1Ac resistance. The larvae fed on a diet containing the Cry1Ac toxin showed no significant change in the expression of the <i>Pxpolycalin</i> gene in a short term. Furthermore, the knockout of <i>polycalin</i> and ATP-binding cassette transporter subfamily C2 <i>(ABCC2)</i> genes separately by CRISPR/Cas9 technology resulted in resistance to decreased susceptibility to Cry1Ac toxin. Our results provide new insights into the potential role of polycalin and ABCC2 proteins in Cry1Ac resistance and the mechanism underlying the resistance of insects to <i>Bt</i> toxins. |
| first_indexed | 2024-03-11T04:28:23Z |
| format | Article |
| id | doaj.art-bc1dd27f78904e0d991fd83a1381b12d |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-03-11T04:28:23Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
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| series | Toxins |
| spelling | doaj.art-bc1dd27f78904e0d991fd83a1381b12d2023-11-17T21:38:52ZengMDPI AGToxins2072-66512023-04-0115427310.3390/toxins15040273Analysis of the Effect of <i>Plutella xylostella Polycalin</i> and <i>ABCC2</i> Transporter on Cry1Ac Susceptibility by CRISPR/Cas9-Mediated KnockoutLei Xiong0Zhaoxia Liu1Jingge Li2Shuyuan Yao3Zeyun Li4Xuanhao Chen5Lingling Shen6Zhen Zhang7Yongbin Li8Qing Hou9Yuhang Zhang10Minsheng You11Zhiguang Yuchi12Shijun You13State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaSchool of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaMany insects, including the <i>Plutella xylostella</i> (L.), have developed varying degrees of resistance to many insecticides, including <i>Bacillus thuringiensis</i> (<i>Bt</i>) toxins, the bioinsecticides derived from <i>Bt</i>. The polycalin protein is one of the potential receptors for <i>Bt</i> toxins, and previous studies have confirmed that the Cry1Ac toxin can bind to the polycalin protein of <i>P. xylostella</i>, but whether polycalin is associated with the resistance of <i>Bt</i> toxins remains controversial. In this study, we compared the midgut of larvae from Cry1Ac-susceptible and -resistant strains, and found that the expression of the <i>Pxpolycalin</i> gene was largely reduced in the midgut of the resistant strains. Moreover, the spatial and temporal expression patterns of <i>Pxpolycalin</i> showed that it was mainly expressed in the larval stage and midgut tissue. However, genetic linkage experiments showed that the <i>Pxpolycalin</i> gene and its transcript level were not linked to Cry1Ac resistance, whereas both the <i>PxABCC2</i> gene and its transcript levels were linked to Cry1Ac resistance. The larvae fed on a diet containing the Cry1Ac toxin showed no significant change in the expression of the <i>Pxpolycalin</i> gene in a short term. Furthermore, the knockout of <i>polycalin</i> and ATP-binding cassette transporter subfamily C2 <i>(ABCC2)</i> genes separately by CRISPR/Cas9 technology resulted in resistance to decreased susceptibility to Cry1Ac toxin. Our results provide new insights into the potential role of polycalin and ABCC2 proteins in Cry1Ac resistance and the mechanism underlying the resistance of insects to <i>Bt</i> toxins.https://www.mdpi.com/2072-6651/15/4/273polycalinABCC2<i>Bacillus thuringiensis</i>CRISPR/Cas9 |
| spellingShingle | Lei Xiong Zhaoxia Liu Jingge Li Shuyuan Yao Zeyun Li Xuanhao Chen Lingling Shen Zhen Zhang Yongbin Li Qing Hou Yuhang Zhang Minsheng You Zhiguang Yuchi Shijun You Analysis of the Effect of <i>Plutella xylostella Polycalin</i> and <i>ABCC2</i> Transporter on Cry1Ac Susceptibility by CRISPR/Cas9-Mediated Knockout Toxins polycalin ABCC2 <i>Bacillus thuringiensis</i> CRISPR/Cas9 |
| title | Analysis of the Effect of <i>Plutella xylostella Polycalin</i> and <i>ABCC2</i> Transporter on Cry1Ac Susceptibility by CRISPR/Cas9-Mediated Knockout |
| title_full | Analysis of the Effect of <i>Plutella xylostella Polycalin</i> and <i>ABCC2</i> Transporter on Cry1Ac Susceptibility by CRISPR/Cas9-Mediated Knockout |
| title_fullStr | Analysis of the Effect of <i>Plutella xylostella Polycalin</i> and <i>ABCC2</i> Transporter on Cry1Ac Susceptibility by CRISPR/Cas9-Mediated Knockout |
| title_full_unstemmed | Analysis of the Effect of <i>Plutella xylostella Polycalin</i> and <i>ABCC2</i> Transporter on Cry1Ac Susceptibility by CRISPR/Cas9-Mediated Knockout |
| title_short | Analysis of the Effect of <i>Plutella xylostella Polycalin</i> and <i>ABCC2</i> Transporter on Cry1Ac Susceptibility by CRISPR/Cas9-Mediated Knockout |
| title_sort | analysis of the effect of i plutella xylostella polycalin i and i abcc2 i transporter on cry1ac susceptibility by crispr cas9 mediated knockout |
| topic | polycalin ABCC2 <i>Bacillus thuringiensis</i> CRISPR/Cas9 |
| url | https://www.mdpi.com/2072-6651/15/4/273 |
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