Development of an effective dissociation protocol for isolating mesenchymal stem cells from bovine intermuscular adipose tissues
Intermuscular fat is essential for enhancing the flavor and texture of cultured meat. Mesenchymal stem cells derived from intermuscular adipose tissues are a source of intermuscular fat. Therefore, as a step towards developing a platform to derive intermuscular fat from mesenchymal stem cells (MSCs)...
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| Format: | Article |
| Language: | English |
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The Korean Society of Animal Reproduction and Biotechnology
2023-03-01
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| Series: | Journal of Animal Reproduction and Biotechnology |
| Subjects: | |
| Online Access: | https://www.e-jarb.org/journal/view.html?uid=2648&vmd=Full |
| _version_ | 1797854822388465664 |
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| author | Jeong Min Lee Hyun Lee Seung Tae Lee |
| author_facet | Jeong Min Lee Hyun Lee Seung Tae Lee |
| author_sort | Jeong Min Lee |
| collection | DOAJ |
| description | Intermuscular fat is essential for enhancing the flavor and texture of cultured meat. Mesenchymal stem cells derived from intermuscular adipose tissues are a source of intermuscular fat. Therefore, as a step towards developing a platform to derive intermuscular fat from mesenchymal stem cells (MSCs) for insertion between myofibrils in cultured beef, an advanced protocol of intermuscular adipose tissue dissociation effective to the isolation of MSCs from intermuscular adipose tissues was developed in cattle. To accomplish this, physical steps were added to the enzymatic dissociation of intermuscular adipose tissues, and the MSCs were established from primary cells dissociated with physical step-free and step-added enzymatic dissociation protocols. The application of a physical step (intensive shaking up) at 5 minutes intervals during enzymatic dissociation resulted in the greatest number of primary cells derived from intermuscular adipose tissues, showed effective formation of colony forming units-fibroblasts (CFU-Fs) from the retrieved primary cells, and generated MSCs with no increase in doubling time. Thus, this protocol will contribute to the stable supply of good quality adipose-derived mesenchymal stem cells (ADMSCs) as a fat source for the production of marbled cultured beef. |
| first_indexed | 2024-04-09T20:13:34Z |
| format | Article |
| id | doaj.art-bc1e53e02e6e41fdb60492e49bba7fc0 |
| institution | Directory Open Access Journal |
| issn | 2671-4639 2671-4663 |
| language | English |
| last_indexed | 2024-04-09T20:13:34Z |
| publishDate | 2023-03-01 |
| publisher | The Korean Society of Animal Reproduction and Biotechnology |
| record_format | Article |
| series | Journal of Animal Reproduction and Biotechnology |
| spelling | doaj.art-bc1e53e02e6e41fdb60492e49bba7fc02023-03-31T15:03:41ZengThe Korean Society of Animal Reproduction and BiotechnologyJournal of Animal Reproduction and Biotechnology2671-46392671-46632023-03-01381101610.12750/JARB.38.1.10Development of an effective dissociation protocol for isolating mesenchymal stem cells from bovine intermuscular adipose tissuesJeong Min Lee0https://orcid.org/0000-0002-1894-0629Hyun Lee1https://orcid.org/0000-0002-1090-3479Seung Tae Lee2https://orcid.org/0000-0002-8952-3881Department of Applied Animal Science, Kangwon National University, Chuncheon 24341, KoreaDepartment of Applied Animal Science, Kangwon National University, Chuncheon 24341, KoreaDepartment of Applied Animal Science, Kangwon National University, Chuncheon 24341, KoreaIntermuscular fat is essential for enhancing the flavor and texture of cultured meat. Mesenchymal stem cells derived from intermuscular adipose tissues are a source of intermuscular fat. Therefore, as a step towards developing a platform to derive intermuscular fat from mesenchymal stem cells (MSCs) for insertion between myofibrils in cultured beef, an advanced protocol of intermuscular adipose tissue dissociation effective to the isolation of MSCs from intermuscular adipose tissues was developed in cattle. To accomplish this, physical steps were added to the enzymatic dissociation of intermuscular adipose tissues, and the MSCs were established from primary cells dissociated with physical step-free and step-added enzymatic dissociation protocols. The application of a physical step (intensive shaking up) at 5 minutes intervals during enzymatic dissociation resulted in the greatest number of primary cells derived from intermuscular adipose tissues, showed effective formation of colony forming units-fibroblasts (CFU-Fs) from the retrieved primary cells, and generated MSCs with no increase in doubling time. Thus, this protocol will contribute to the stable supply of good quality adipose-derived mesenchymal stem cells (ADMSCs) as a fat source for the production of marbled cultured beef.https://www.e-jarb.org/journal/view.html?uid=2648&vmd=Fullbovine adipose tissue-derived stem cellsculture meatintermuscular adipose tissue-derived stem cellsisolation procedurephysical step |
| spellingShingle | Jeong Min Lee Hyun Lee Seung Tae Lee Development of an effective dissociation protocol for isolating mesenchymal stem cells from bovine intermuscular adipose tissues Journal of Animal Reproduction and Biotechnology bovine adipose tissue-derived stem cells culture meat intermuscular adipose tissue-derived stem cells isolation procedure physical step |
| title | Development of an effective dissociation protocol for isolating mesenchymal stem cells from bovine intermuscular adipose tissues |
| title_full | Development of an effective dissociation protocol for isolating mesenchymal stem cells from bovine intermuscular adipose tissues |
| title_fullStr | Development of an effective dissociation protocol for isolating mesenchymal stem cells from bovine intermuscular adipose tissues |
| title_full_unstemmed | Development of an effective dissociation protocol for isolating mesenchymal stem cells from bovine intermuscular adipose tissues |
| title_short | Development of an effective dissociation protocol for isolating mesenchymal stem cells from bovine intermuscular adipose tissues |
| title_sort | development of an effective dissociation protocol for isolating mesenchymal stem cells from bovine intermuscular adipose tissues |
| topic | bovine adipose tissue-derived stem cells culture meat intermuscular adipose tissue-derived stem cells isolation procedure physical step |
| url | https://www.e-jarb.org/journal/view.html?uid=2648&vmd=Full |
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