Lachnospiraceae shift in the microbial community of mice faecal sample effects on water immersion restraint stress
Abstracts Stress, including both psychological and physical stimulation, can cause changes in the microbiota and mucosal function of the gastrointestinal system. There are few research studies available about the faecal microbiota changes after stress, such as water immersion restraint stress (WIRS)...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2017-04-01
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| Series: | AMB Express |
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| Online Access: | http://link.springer.com/article/10.1186/s13568-017-0383-4 |
| _version_ | 1818148478963941376 |
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| author | Shiyin Li Zelin Wang Yun Yang Sha Yang Chenchen Yao Kaiyun Liu Sixin Cui Quanming Zou Hongwu Sun Gang Guo |
| author_facet | Shiyin Li Zelin Wang Yun Yang Sha Yang Chenchen Yao Kaiyun Liu Sixin Cui Quanming Zou Hongwu Sun Gang Guo |
| author_sort | Shiyin Li |
| collection | DOAJ |
| description | Abstracts Stress, including both psychological and physical stimulation, can cause changes in the microbiota and mucosal function of the gastrointestinal system. There are few research studies available about the faecal microbiota changes after stress, such as water immersion restraint stress (WIRS). Therefore, in this study, we focused on analysing the composition changes of faecal microbiota in WIRS mice. The WIRS model, in which Blab/c mice were immersed in 21 ± 2 °C water for 4 h each day for 14 days, was established. Behavioural changes, the serum levels of corticosterone, IFN-γ and IL-17 and gastric mucosal injury were also assessed. Ten faecal microbiota samples were detected by Illumina Miseq sequencing of the 16S rRNA genes from 367205 characterised sequences. Finally, we find significant differences in the faecal microbiota composition between the control and the WIRS groups. There was an obvious increase in Lachnospiraceae in the WIRS mice (p = 0.0286, p < 0.05), which is associated with human diseases, such as ulcerative colitis, Crohn’s and celiac disease. Our research indicates that stress changes in the faecal microbiota. These results suggest that observing shifts of the intestinal microbiota is a promising method to explore the mechanism of the stress associated with gastrointestinal diseases and to provide us with a better understanding of the relationship between the microbiota and disease. |
| first_indexed | 2024-12-11T12:51:47Z |
| format | Article |
| id | doaj.art-bc1f580df2c24767bf6ff50356a1432a |
| institution | Directory Open Access Journal |
| issn | 2191-0855 |
| language | English |
| last_indexed | 2024-12-11T12:51:47Z |
| publishDate | 2017-04-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | AMB Express |
| spelling | doaj.art-bc1f580df2c24767bf6ff50356a1432a2022-12-22T01:06:40ZengSpringerOpenAMB Express2191-08552017-04-017111110.1186/s13568-017-0383-4Lachnospiraceae shift in the microbial community of mice faecal sample effects on water immersion restraint stressShiyin Li0Zelin Wang1Yun Yang2Sha Yang3Chenchen Yao4Kaiyun Liu5Sixin Cui6Quanming Zou7Hongwu Sun8Gang Guo9National Engineering Research Center of Immunological Products, Department of Microbiology and Biochemical Pharmacy, College of Pharmacy, Third Military Medical University of PLANational Engineering Research Center of Immunological Products, Department of Microbiology and Biochemical Pharmacy, College of Pharmacy, Third Military Medical University of PLANational Engineering Research Center of Immunological Products, Department of Microbiology and Biochemical Pharmacy, College of Pharmacy, Third Military Medical University of PLANational Engineering Research Center of Immunological Products, Department of Microbiology and Biochemical Pharmacy, College of Pharmacy, Third Military Medical University of PLANational Engineering Research Center of Immunological Products, Department of Microbiology and Biochemical Pharmacy, College of Pharmacy, Third Military Medical University of PLANational Engineering Research Center of Immunological Products, Department of Microbiology and Biochemical Pharmacy, College of Pharmacy, Third Military Medical University of PLANational Engineering Research Center of Immunological Products, Department of Microbiology and Biochemical Pharmacy, College of Pharmacy, Third Military Medical University of PLANational Engineering Research Center of Immunological Products, Department of Microbiology and Biochemical Pharmacy, College of Pharmacy, Third Military Medical University of PLANational Engineering Research Center of Immunological Products, Department of Microbiology and Biochemical Pharmacy, College of Pharmacy, Third Military Medical University of PLANational Engineering Research Center of Immunological Products, Department of Microbiology and Biochemical Pharmacy, College of Pharmacy, Third Military Medical University of PLAAbstracts Stress, including both psychological and physical stimulation, can cause changes in the microbiota and mucosal function of the gastrointestinal system. There are few research studies available about the faecal microbiota changes after stress, such as water immersion restraint stress (WIRS). Therefore, in this study, we focused on analysing the composition changes of faecal microbiota in WIRS mice. The WIRS model, in which Blab/c mice were immersed in 21 ± 2 °C water for 4 h each day for 14 days, was established. Behavioural changes, the serum levels of corticosterone, IFN-γ and IL-17 and gastric mucosal injury were also assessed. Ten faecal microbiota samples were detected by Illumina Miseq sequencing of the 16S rRNA genes from 367205 characterised sequences. Finally, we find significant differences in the faecal microbiota composition between the control and the WIRS groups. There was an obvious increase in Lachnospiraceae in the WIRS mice (p = 0.0286, p < 0.05), which is associated with human diseases, such as ulcerative colitis, Crohn’s and celiac disease. Our research indicates that stress changes in the faecal microbiota. These results suggest that observing shifts of the intestinal microbiota is a promising method to explore the mechanism of the stress associated with gastrointestinal diseases and to provide us with a better understanding of the relationship between the microbiota and disease.http://link.springer.com/article/10.1186/s13568-017-0383-4Water immersion restraint stressFaecal microbiota16s rRNAMicrobial communityIllumina Miseq sequencing |
| spellingShingle | Shiyin Li Zelin Wang Yun Yang Sha Yang Chenchen Yao Kaiyun Liu Sixin Cui Quanming Zou Hongwu Sun Gang Guo Lachnospiraceae shift in the microbial community of mice faecal sample effects on water immersion restraint stress AMB Express Water immersion restraint stress Faecal microbiota 16s rRNA Microbial community Illumina Miseq sequencing |
| title | Lachnospiraceae shift in the microbial community of mice faecal sample effects on water immersion restraint stress |
| title_full | Lachnospiraceae shift in the microbial community of mice faecal sample effects on water immersion restraint stress |
| title_fullStr | Lachnospiraceae shift in the microbial community of mice faecal sample effects on water immersion restraint stress |
| title_full_unstemmed | Lachnospiraceae shift in the microbial community of mice faecal sample effects on water immersion restraint stress |
| title_short | Lachnospiraceae shift in the microbial community of mice faecal sample effects on water immersion restraint stress |
| title_sort | lachnospiraceae shift in the microbial community of mice faecal sample effects on water immersion restraint stress |
| topic | Water immersion restraint stress Faecal microbiota 16s rRNA Microbial community Illumina Miseq sequencing |
| url | http://link.springer.com/article/10.1186/s13568-017-0383-4 |
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