A novel phytopathogen Erwinia sorbitola sp. nov., isolated from the feces of ruddy shelducks
The species in the genus Erwinia are Gram-stain-negative, facultatively anaerobic, motile, and rod-shaped. Most species in the genus Erwinia are phytopathogens. Also, Erwinia persicina was involved in several human infections. Based on the reverse microbial etiology principles, it is worth analyzing...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-02-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2023.1109634/full |
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| author | Yuanmeihui Tao Yajun Ge Yajun Ge Jing Yang Jing Yang Weitao Song Dong Jin Dong Jin Hong Lin Han Zheng Han Zheng Shan Lu Shan Lu Wenbo Luo Yuyuan Huang Zhenhong Zhuang Jianguo Xu Jianguo Xu Jianguo Xu |
| author_facet | Yuanmeihui Tao Yajun Ge Yajun Ge Jing Yang Jing Yang Weitao Song Dong Jin Dong Jin Hong Lin Han Zheng Han Zheng Shan Lu Shan Lu Wenbo Luo Yuyuan Huang Zhenhong Zhuang Jianguo Xu Jianguo Xu Jianguo Xu |
| author_sort | Yuanmeihui Tao |
| collection | DOAJ |
| description | The species in the genus Erwinia are Gram-stain-negative, facultatively anaerobic, motile, and rod-shaped. Most species in the genus Erwinia are phytopathogens. Also, Erwinia persicina was involved in several human infections. Based on the reverse microbial etiology principles, it is worth analyzing the pathogenicity of species in this genus. In this study, we isolated and sequenced two species of Erwinia. Phylogenetic, phenotypic, biochemical, and chemotaxonomic analyses were performed to identify its taxonomy position. The virulence tests on plant leaves and pear fruits were used to identify the plant pathogenicity of two species of Erwinia. Bioinformatic methods predicted the possible pathogenic determinants based on the genome sequence. Meanwhile, adhesion, invasion, and cytotoxicity assays on RAW 264.7 cells were applied to identify animal pathogenicity. We isolated two Gram-stain-negative, facultatively anaerobic, motile, and rod-shaped strains from the feces of ruddy shelducks in the Tibet Plateau of China, designated J780T and J316. Distinct phylogenetic, genomic, phenotypic, biochemical, and chemotaxonomic characters of J780T and J316 identified they were novel species and belonged to the genus Erwinia, for which the name Erwinia sorbitola sp. nov. was proposed, the type strain was J780T (= CGMCC 1.17334T = GDMCC 1.1666T = JCM 33839T). Virulence tests showed blight and rot on the leaves and pear fruits confirmed Erwinia sorbitola sp. nov. was a phytopathogen. Predicted gene clusters of motility, biofilm formation, exopolysaccharides, stress survival, siderophores, and Type VI secretion system might be the causes of pathogenicity. In addition, predicted polysaccharide biosynthesis gene clusters on the genome sequence, and the high capacity for adhesion, invasion, and cytotoxicity to animal cells confirmed it has pathogenicity on animals. In conclusion, we isolated and identified a novel phytopathogen Erwinia sorbitola sp. nov. in ruddy shelducks. A predefined pathogen is beneficial for preventing from suffering potential economic losses caused by this new pathogen. |
| first_indexed | 2024-04-10T09:59:12Z |
| format | Article |
| id | doaj.art-bc20d6d79e814bd1a2feba5343deea8f |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-04-10T09:59:12Z |
| publishDate | 2023-02-01 |
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| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-bc20d6d79e814bd1a2feba5343deea8f2023-02-16T10:25:18ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882023-02-011310.3389/fcimb.2023.11096341109634A novel phytopathogen Erwinia sorbitola sp. nov., isolated from the feces of ruddy shelducksYuanmeihui Tao0Yajun Ge1Yajun Ge2Jing Yang3Jing Yang4Weitao Song5Dong Jin6Dong Jin7Hong Lin8Han Zheng9Han Zheng10Shan Lu11Shan Lu12Wenbo Luo13Yuyuan Huang14Zhenhong Zhuang15Jianguo Xu16Jianguo Xu17Jianguo Xu18State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping, Beijing, ChinaState Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping, Beijing, ChinaCollege of Basic Medicine, Gansu University of Chinese Medicine, Lanzhou, ChinaState Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping, Beijing, ChinaResearch Units of Discovery of Unknown Bacteria and Function, Chinese Academy of Medical Sciences, Beijing, ChinaKey Laboratory of Pathogenic Fungi and Mycotoxins of Fujian Province, Proteomic Research Center, and School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, ChinaState Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping, Beijing, ChinaResearch Units of Discovery of Unknown Bacteria and Function, Chinese Academy of Medical Sciences, Beijing, ChinaKey Laboratory of Pathogenic Fungi and Mycotoxins of Fujian Province, Proteomic Research Center, and School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, ChinaState Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping, Beijing, ChinaResearch Units of Discovery of Unknown Bacteria and Function, Chinese Academy of Medical Sciences, Beijing, ChinaState Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping, Beijing, ChinaResearch Units of Discovery of Unknown Bacteria and Function, Chinese Academy of Medical Sciences, Beijing, ChinaState Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping, Beijing, ChinaState Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping, Beijing, ChinaKey Laboratory of Pathogenic Fungi and Mycotoxins of Fujian Province, Proteomic Research Center, and School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, ChinaState Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping, Beijing, ChinaResearch Units of Discovery of Unknown Bacteria and Function, Chinese Academy of Medical Sciences, Beijing, ChinaResearch Institute of Public Health, Nankai University, Tianjin, ChinaThe species in the genus Erwinia are Gram-stain-negative, facultatively anaerobic, motile, and rod-shaped. Most species in the genus Erwinia are phytopathogens. Also, Erwinia persicina was involved in several human infections. Based on the reverse microbial etiology principles, it is worth analyzing the pathogenicity of species in this genus. In this study, we isolated and sequenced two species of Erwinia. Phylogenetic, phenotypic, biochemical, and chemotaxonomic analyses were performed to identify its taxonomy position. The virulence tests on plant leaves and pear fruits were used to identify the plant pathogenicity of two species of Erwinia. Bioinformatic methods predicted the possible pathogenic determinants based on the genome sequence. Meanwhile, adhesion, invasion, and cytotoxicity assays on RAW 264.7 cells were applied to identify animal pathogenicity. We isolated two Gram-stain-negative, facultatively anaerobic, motile, and rod-shaped strains from the feces of ruddy shelducks in the Tibet Plateau of China, designated J780T and J316. Distinct phylogenetic, genomic, phenotypic, biochemical, and chemotaxonomic characters of J780T and J316 identified they were novel species and belonged to the genus Erwinia, for which the name Erwinia sorbitola sp. nov. was proposed, the type strain was J780T (= CGMCC 1.17334T = GDMCC 1.1666T = JCM 33839T). Virulence tests showed blight and rot on the leaves and pear fruits confirmed Erwinia sorbitola sp. nov. was a phytopathogen. Predicted gene clusters of motility, biofilm formation, exopolysaccharides, stress survival, siderophores, and Type VI secretion system might be the causes of pathogenicity. In addition, predicted polysaccharide biosynthesis gene clusters on the genome sequence, and the high capacity for adhesion, invasion, and cytotoxicity to animal cells confirmed it has pathogenicity on animals. In conclusion, we isolated and identified a novel phytopathogen Erwinia sorbitola sp. nov. in ruddy shelducks. A predefined pathogen is beneficial for preventing from suffering potential economic losses caused by this new pathogen.https://www.frontiersin.org/articles/10.3389/fcimb.2023.1109634/fullphytopathogenreverse microbial etiologypathogenicity analysisErwinia sorbitolaruddy shelducks |
| spellingShingle | Yuanmeihui Tao Yajun Ge Yajun Ge Jing Yang Jing Yang Weitao Song Dong Jin Dong Jin Hong Lin Han Zheng Han Zheng Shan Lu Shan Lu Wenbo Luo Yuyuan Huang Zhenhong Zhuang Jianguo Xu Jianguo Xu Jianguo Xu A novel phytopathogen Erwinia sorbitola sp. nov., isolated from the feces of ruddy shelducks Frontiers in Cellular and Infection Microbiology phytopathogen reverse microbial etiology pathogenicity analysis Erwinia sorbitola ruddy shelducks |
| title | A novel phytopathogen Erwinia sorbitola sp. nov., isolated from the feces of ruddy shelducks |
| title_full | A novel phytopathogen Erwinia sorbitola sp. nov., isolated from the feces of ruddy shelducks |
| title_fullStr | A novel phytopathogen Erwinia sorbitola sp. nov., isolated from the feces of ruddy shelducks |
| title_full_unstemmed | A novel phytopathogen Erwinia sorbitola sp. nov., isolated from the feces of ruddy shelducks |
| title_short | A novel phytopathogen Erwinia sorbitola sp. nov., isolated from the feces of ruddy shelducks |
| title_sort | novel phytopathogen erwinia sorbitola sp nov isolated from the feces of ruddy shelducks |
| topic | phytopathogen reverse microbial etiology pathogenicity analysis Erwinia sorbitola ruddy shelducks |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2023.1109634/full |
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