Construction of Biocompatible Hydrogel Scaffolds With a Long-Term Drug Release for Facilitating Cartilage Repair
In tissue engineering, hydrogel scaffolds allow various cells to be cultured and grown in vitro and then implanted to repair or replace the damaged areas. Here in this work, kartogenin (KGN), an effectively chondro-inductive non-protein bioactive drug molecule, was incorporated into a composite hydr...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-06-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2022.922032/full |
| _version_ | 1817968159408259072 |
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| author | Wei Zhang Rui Chen Xiong Xu Liang Zhu Yanbin Liu XiaoJie Yu GuoKe Tang GuoKe Tang |
| author_facet | Wei Zhang Rui Chen Xiong Xu Liang Zhu Yanbin Liu XiaoJie Yu GuoKe Tang GuoKe Tang |
| author_sort | Wei Zhang |
| collection | DOAJ |
| description | In tissue engineering, hydrogel scaffolds allow various cells to be cultured and grown in vitro and then implanted to repair or replace the damaged areas. Here in this work, kartogenin (KGN), an effectively chondro-inductive non-protein bioactive drug molecule, was incorporated into a composite hydrogel comprising the positively charged chitosan (CS) and methacrylated gelatin (GelMA) polymers to fabricate appropriate microenvironments of bone marrow mesenchymal stem cells (BMSCs) for cartilage regeneration. Based on the combination of physical chain entanglements and chemical crosslinking effects, the resultant GelMA-CS@KGN composite hydrogels possessed favorable network pores and mechanical strength. In vitro cytotoxicity showed the excellent biocompatibility for facilitating the cell growth, adhesion, proliferation, and differentiation. The long-term sustainable KGN release from the hydrogel scaffolds in situ promoted the chondrogenic differentiation that can be employed as an alternative candidate for cartilage tissue regeneration. |
| first_indexed | 2024-04-13T20:04:25Z |
| format | Article |
| id | doaj.art-bc24ec2d638e47d2bfd00d23de594b8f |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-04-13T20:04:25Z |
| publishDate | 2022-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-bc24ec2d638e47d2bfd00d23de594b8f2022-12-22T02:32:04ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122022-06-011310.3389/fphar.2022.922032922032Construction of Biocompatible Hydrogel Scaffolds With a Long-Term Drug Release for Facilitating Cartilage RepairWei Zhang0Rui Chen1Xiong Xu2Liang Zhu3Yanbin Liu4XiaoJie Yu5GuoKe Tang6GuoKe Tang7Joint Surgery Department, Zhuzhou Central Hospital, Zhuzhou, ChinaDepartment of Orthopedics, Second Affiliated Hospital of Naval Medical University, Shanghai, ChinaDepartment of Graduate, Hebei North University, Zhangjiakou, ChinaDepartment of Orthopedics, Shanghai General Hospital, Shanghai Jiaotong University, Shanghai, ChinaDepartment of Orthopedics, Shanghai General Hospital, Shanghai Jiaotong University, Shanghai, ChinaDepartment of Orthopedics, Hunan Aerospace Hospital, Changsha, ChinaJoint Surgery Department, Zhuzhou Central Hospital, Zhuzhou, ChinaDepartment of Orthopedics, Shanghai General Hospital, Shanghai Jiaotong University, Shanghai, ChinaIn tissue engineering, hydrogel scaffolds allow various cells to be cultured and grown in vitro and then implanted to repair or replace the damaged areas. Here in this work, kartogenin (KGN), an effectively chondro-inductive non-protein bioactive drug molecule, was incorporated into a composite hydrogel comprising the positively charged chitosan (CS) and methacrylated gelatin (GelMA) polymers to fabricate appropriate microenvironments of bone marrow mesenchymal stem cells (BMSCs) for cartilage regeneration. Based on the combination of physical chain entanglements and chemical crosslinking effects, the resultant GelMA-CS@KGN composite hydrogels possessed favorable network pores and mechanical strength. In vitro cytotoxicity showed the excellent biocompatibility for facilitating the cell growth, adhesion, proliferation, and differentiation. The long-term sustainable KGN release from the hydrogel scaffolds in situ promoted the chondrogenic differentiation that can be employed as an alternative candidate for cartilage tissue regeneration.https://www.frontiersin.org/articles/10.3389/fphar.2022.922032/fullBMSCscartilage regenerationhydrogel scaffoldKGNlong-term release |
| spellingShingle | Wei Zhang Rui Chen Xiong Xu Liang Zhu Yanbin Liu XiaoJie Yu GuoKe Tang GuoKe Tang Construction of Biocompatible Hydrogel Scaffolds With a Long-Term Drug Release for Facilitating Cartilage Repair Frontiers in Pharmacology BMSCs cartilage regeneration hydrogel scaffold KGN long-term release |
| title | Construction of Biocompatible Hydrogel Scaffolds With a Long-Term Drug Release for Facilitating Cartilage Repair |
| title_full | Construction of Biocompatible Hydrogel Scaffolds With a Long-Term Drug Release for Facilitating Cartilage Repair |
| title_fullStr | Construction of Biocompatible Hydrogel Scaffolds With a Long-Term Drug Release for Facilitating Cartilage Repair |
| title_full_unstemmed | Construction of Biocompatible Hydrogel Scaffolds With a Long-Term Drug Release for Facilitating Cartilage Repair |
| title_short | Construction of Biocompatible Hydrogel Scaffolds With a Long-Term Drug Release for Facilitating Cartilage Repair |
| title_sort | construction of biocompatible hydrogel scaffolds with a long term drug release for facilitating cartilage repair |
| topic | BMSCs cartilage regeneration hydrogel scaffold KGN long-term release |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2022.922032/full |
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