Induction of a Hypersensitive Response by Chimeric Helicase Sequences of Tobamoviruses U1 and Ob in N-Carrying Tobacco
Recently, the helicase domain of the Tobacco mosaic virus (TMV)-U1 replicase proteins (designated MOREHEL:U1) was identified as the elicitor of the N gene-mediated hypersensitive response (HR) in tobacco. In this study, we used agroinfiltration to express the equivalent MOREHEL domain of the non-HR-...
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2001-09-01
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| Series: | Molecular Plant-Microbe Interactions |
| Subjects: | |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI.2001.14.9.1086 |
| _version_ | 1811264535163568128 |
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| author | Truus E. M. Abbink Johan de Vogel John F. Bol Huub J. M. Linthorst |
| author_facet | Truus E. M. Abbink Johan de Vogel John F. Bol Huub J. M. Linthorst |
| author_sort | Truus E. M. Abbink |
| collection | DOAJ |
| description | Recently, the helicase domain of the Tobacco mosaic virus (TMV)-U1 replicase proteins (designated MOREHEL:U1) was identified as the elicitor of the N gene-mediated hypersensitive response (HR) in tobacco. In this study, we used agroinfiltration to express the equivalent MOREHEL domain of the non-HR-inducing tobamovirus strain TMV-Ob. It appeared that this MOREHEL:Ob sequence did not elicit a HR in N gene-carrying tobacco. Both MOREHEL sequences were divided into eight subdomains, and chimeras of MOREHEL sequences from U1 and Ob were constructed. Expression of these chimeric MOREHEL sequences revealed that, in the TMV-U1 MOREHEL sequence, at least four domains involved in full HR induction were present. The presence of at least three of these four domains seems a minimal requirement for HR induction. Two additional domains may play a minor role in HR induction. To study the elicitor function of the chimeras during the TMV life cycle, chimeric MOREHEL domains were introduced into full-length TMV cDNA clones. These constructs, however, were unable to establish an infection in Nicotiana benthamiana or Nicotiana tabacum plants. |
| first_indexed | 2024-04-12T20:06:05Z |
| format | Article |
| id | doaj.art-bc267baae19446229625aa1dfb2efffc |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-12T20:06:05Z |
| publishDate | 2001-09-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-bc267baae19446229625aa1dfb2efffc2022-12-22T03:18:24ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062001-09-011491086109510.1094/MPMI.2001.14.9.1086Induction of a Hypersensitive Response by Chimeric Helicase Sequences of Tobamoviruses U1 and Ob in N-Carrying TobaccoTruus E. M. AbbinkJohan de VogelJohn F. BolHuub J. M. LinthorstRecently, the helicase domain of the Tobacco mosaic virus (TMV)-U1 replicase proteins (designated MOREHEL:U1) was identified as the elicitor of the N gene-mediated hypersensitive response (HR) in tobacco. In this study, we used agroinfiltration to express the equivalent MOREHEL domain of the non-HR-inducing tobamovirus strain TMV-Ob. It appeared that this MOREHEL:Ob sequence did not elicit a HR in N gene-carrying tobacco. Both MOREHEL sequences were divided into eight subdomains, and chimeras of MOREHEL sequences from U1 and Ob were constructed. Expression of these chimeric MOREHEL sequences revealed that, in the TMV-U1 MOREHEL sequence, at least four domains involved in full HR induction were present. The presence of at least three of these four domains seems a minimal requirement for HR induction. Two additional domains may play a minor role in HR induction. To study the elicitor function of the chimeras during the TMV life cycle, chimeric MOREHEL domains were introduced into full-length TMV cDNA clones. These constructs, however, were unable to establish an infection in Nicotiana benthamiana or Nicotiana tabacum plants.https://apsjournals.apsnet.org/doi/10.1094/MPMI.2001.14.9.1086avirulencegene for geneprotein-protein interactionsignal transduction |
| spellingShingle | Truus E. M. Abbink Johan de Vogel John F. Bol Huub J. M. Linthorst Induction of a Hypersensitive Response by Chimeric Helicase Sequences of Tobamoviruses U1 and Ob in N-Carrying Tobacco Molecular Plant-Microbe Interactions avirulence gene for gene protein-protein interaction signal transduction |
| title | Induction of a Hypersensitive Response by Chimeric Helicase Sequences of Tobamoviruses U1 and Ob in N-Carrying Tobacco |
| title_full | Induction of a Hypersensitive Response by Chimeric Helicase Sequences of Tobamoviruses U1 and Ob in N-Carrying Tobacco |
| title_fullStr | Induction of a Hypersensitive Response by Chimeric Helicase Sequences of Tobamoviruses U1 and Ob in N-Carrying Tobacco |
| title_full_unstemmed | Induction of a Hypersensitive Response by Chimeric Helicase Sequences of Tobamoviruses U1 and Ob in N-Carrying Tobacco |
| title_short | Induction of a Hypersensitive Response by Chimeric Helicase Sequences of Tobamoviruses U1 and Ob in N-Carrying Tobacco |
| title_sort | induction of a hypersensitive response by chimeric helicase sequences of tobamoviruses u1 and ob in n carrying tobacco |
| topic | avirulence gene for gene protein-protein interaction signal transduction |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI.2001.14.9.1086 |
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