Season does not have a deleterious effect on proportions of stallion seminal plasma proteins
The mechanism by which the content of the major groups of seminal plasma proteins in stallion semen changes between the breeding and non-breeding seasons remains unknown. Here, we investigated the proportions of non-heparin-binding, phosphorylcholine-binding, and heparin-binding proteins in seminal...
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| Format: | Artikel |
| Sprache: | English |
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The Society for Reproduction and Development
2020-02-01
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| Schriftenreihe: | The Journal of Reproduction and Development |
| Schlagworte: | |
| Online Zugang: | https://www.jstage.jst.go.jp/article/jrd/66/3/66_2019-072/_pdf/-char/en |
| _version_ | 1827752625912676352 |
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| author | Anders JOHANNISSON Essraa M. AL-ESSAWE Anas Kh. AL-SAFFAR Saeid KARKEHABADI Isabel LIMA-VERDE Manuela WULF Christine AURICH Jane M. MORRELL |
| author_facet | Anders JOHANNISSON Essraa M. AL-ESSAWE Anas Kh. AL-SAFFAR Saeid KARKEHABADI Isabel LIMA-VERDE Manuela WULF Christine AURICH Jane M. MORRELL |
| author_sort | Anders JOHANNISSON |
| collection | DOAJ |
| description | The mechanism by which the content of the major groups of seminal plasma proteins in stallion semen changes between the breeding and non-breeding seasons remains unknown. Here, we investigated the proportions of non-heparin-binding, phosphorylcholine-binding, and heparin-binding proteins in seminal plasma with the aim of relating them to sperm quality and testosterone levels in good and bad freezer stallions. Only minor variations in the major protein groups were found between the breeding and non-breeding seasons. In the non-breeding season, a higher content of a subset of non-heparin binding proteins as well as of heparin-binding proteins was found. Analysis of semen characteristics revealed a somewhat contrasting picture. While only minor variations in sperm kinematics and sperm morphology were found between seasons, the flow-cytometric measurements of mitochondrial membrane potential and also, to some extent, reactive oxygen species production indicated lower sperm quality in the breeding season. Chromatin integrity and testosterone levels were unchanged between seasons. The results suggest that stallion ejaculates could be used year-round for freezing, since only minor differences in protein composition exist between the breeding and non-breeding seasons, as well as between good and bad freezers. In addition, sperm quality is not impaired during the non-breeding season. |
| first_indexed | 2024-03-11T07:24:52Z |
| format | Article |
| id | doaj.art-bc274a95fd8d41328008d9d24dd2a46c |
| institution | Directory Open Access Journal |
| issn | 0916-8818 1348-4400 |
| language | English |
| last_indexed | 2024-03-11T07:24:52Z |
| publishDate | 2020-02-01 |
| publisher | The Society for Reproduction and Development |
| record_format | Article |
| series | The Journal of Reproduction and Development |
| spelling | doaj.art-bc274a95fd8d41328008d9d24dd2a46c2023-11-17T07:42:39ZengThe Society for Reproduction and DevelopmentThe Journal of Reproduction and Development0916-88181348-44002020-02-0166321522110.1262/jrd.2019-072jrdSeason does not have a deleterious effect on proportions of stallion seminal plasma proteinsAnders JOHANNISSON0Essraa M. AL-ESSAWE1Anas Kh. AL-SAFFAR2Saeid KARKEHABADI3Isabel LIMA-VERDE4Manuela WULF5Christine AURICH6Jane M. MORRELL7Swedish University of Agricultural Sciences, Clinical Sciences, Uppsala 75007, SwedenSwedish University of Agricultural Sciences, Clinical Sciences, Uppsala 75007, SwedenGastroenterology and Hepatology Unit, Department of Medical Sciences, Uppsala University, Uppsala 75185, SwedenSwedish University of Agricultural Sciences, Molecular Sciences, Uppsala 75007, SwedenSwedish University of Agricultural Sciences, Clinical Sciences, Uppsala 75007, SwedenGraf Lehndorff Institute for Equine Sciences, Vetmeduni, Vienna, Austria, and Neustadt (Dosse) 16845, GermanyArtificial Insemination and Embryo Transfer, Department for Small Animals and Horses, Vetmeduni Vienna, Vienna 1210, AustriaSwedish University of Agricultural Sciences, Clinical Sciences, Uppsala 75007, SwedenThe mechanism by which the content of the major groups of seminal plasma proteins in stallion semen changes between the breeding and non-breeding seasons remains unknown. Here, we investigated the proportions of non-heparin-binding, phosphorylcholine-binding, and heparin-binding proteins in seminal plasma with the aim of relating them to sperm quality and testosterone levels in good and bad freezer stallions. Only minor variations in the major protein groups were found between the breeding and non-breeding seasons. In the non-breeding season, a higher content of a subset of non-heparin binding proteins as well as of heparin-binding proteins was found. Analysis of semen characteristics revealed a somewhat contrasting picture. While only minor variations in sperm kinematics and sperm morphology were found between seasons, the flow-cytometric measurements of mitochondrial membrane potential and also, to some extent, reactive oxygen species production indicated lower sperm quality in the breeding season. Chromatin integrity and testosterone levels were unchanged between seasons. The results suggest that stallion ejaculates could be used year-round for freezing, since only minor differences in protein composition exist between the breeding and non-breeding seasons, as well as between good and bad freezers. In addition, sperm quality is not impaired during the non-breeding season.https://www.jstage.jst.go.jp/article/jrd/66/3/66_2019-072/_pdf/-char/enchromatographycytometryprotein fractionsseasonal changesstallion |
| spellingShingle | Anders JOHANNISSON Essraa M. AL-ESSAWE Anas Kh. AL-SAFFAR Saeid KARKEHABADI Isabel LIMA-VERDE Manuela WULF Christine AURICH Jane M. MORRELL Season does not have a deleterious effect on proportions of stallion seminal plasma proteins The Journal of Reproduction and Development chromatography cytometry protein fractions seasonal changes stallion |
| title | Season does not have a deleterious effect on proportions of stallion seminal plasma proteins |
| title_full | Season does not have a deleterious effect on proportions of stallion seminal plasma proteins |
| title_fullStr | Season does not have a deleterious effect on proportions of stallion seminal plasma proteins |
| title_full_unstemmed | Season does not have a deleterious effect on proportions of stallion seminal plasma proteins |
| title_short | Season does not have a deleterious effect on proportions of stallion seminal plasma proteins |
| title_sort | season does not have a deleterious effect on proportions of stallion seminal plasma proteins |
| topic | chromatography cytometry protein fractions seasonal changes stallion |
| url | https://www.jstage.jst.go.jp/article/jrd/66/3/66_2019-072/_pdf/-char/en |
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