Antibody titers in Group O platelet donors
Background and Objectives: The occurrence of hemolysis due to transfusion of ABO plasma-incompatible platelets (PLTs) is challenging. There has been no consensus for critical antibody titers in the transfusion community. This study was conducted to understand the trends of anti-A and anti-B antibody...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2017-01-01
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| Series: | Asian Journal of Transfusion Science |
| Subjects: | |
| Online Access: | http://www.ajts.org/article.asp?issn=0973-6247;year=2017;volume=11;issue=1;spage=22;epage=27;aulast=Tendulkar |
| _version_ | 1818669810273222656 |
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| author | Anita Amar Tendulkar Puneet Ashok Jain Sanjay Velaye |
| author_facet | Anita Amar Tendulkar Puneet Ashok Jain Sanjay Velaye |
| author_sort | Anita Amar Tendulkar |
| collection | DOAJ |
| description | Background and Objectives: The occurrence of hemolysis due to transfusion of ABO plasma-incompatible platelets (PLTs) is challenging. There has been no consensus for critical antibody titers in the transfusion community. This study was conducted to understand the trends of anti-A and anti-B antibody titer levels in O group donors and to identify any specific patterns of distribution in relation to age and gender.
Materials and Methods: A total of 1635 Group O PLT donors were randomly selected for this prospective study. Serial 2-fold doubling dilutions were prepared for each sample to calculate the titer of anti-A and anti-B in a standard 96 well micro-plate. Tube technique was used for comparison with the microplate method for 100 samples.
Results: Out of 1635 donors, 1430 (87.46%) were males and 205 (12.54%) were females. The median titer for anti-A and anti-B was 128 with range from 4 to 2048. Spearman's correlation coefficient for microplate versus tube technique was estimated to be 0.803 (P < 0.01, two-tailed). 57.12% and 51.19% of all donors had titers ≥128 for anti-A and anti-B, respectively. The geometric mean of anti-A and anti-B was 155.7 and 137.28, respectively. The titers were significantly higher (P < 0.001) in female donors. An inverse relation between titer levels and age was seen.
Conclusion: Microplate can be used to perform titers in resource-constrained settings. Screening for critical titers in O group donors is essential as they are more implicated in hemolytic transfusion reactions. In the absence of a global consensus on this topic, institutes may need to formulate their own guidelines on handling ABO plasma-incompatible PLT transfusions. |
| first_indexed | 2024-12-17T06:58:08Z |
| format | Article |
| id | doaj.art-bc28aa0462b843c9b89b00177c36e258 |
| institution | Directory Open Access Journal |
| issn | 0973-6247 1998-3565 |
| language | English |
| last_indexed | 2024-12-17T06:58:08Z |
| publishDate | 2017-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Journal of Transfusion Science |
| spelling | doaj.art-bc28aa0462b843c9b89b00177c36e2582022-12-21T21:59:22ZengWolters Kluwer Medknow PublicationsAsian Journal of Transfusion Science0973-62471998-35652017-01-01111222710.4103/0973-6247.200765Antibody titers in Group O platelet donorsAnita Amar TendulkarPuneet Ashok JainSanjay VelayeBackground and Objectives: The occurrence of hemolysis due to transfusion of ABO plasma-incompatible platelets (PLTs) is challenging. There has been no consensus for critical antibody titers in the transfusion community. This study was conducted to understand the trends of anti-A and anti-B antibody titer levels in O group donors and to identify any specific patterns of distribution in relation to age and gender. Materials and Methods: A total of 1635 Group O PLT donors were randomly selected for this prospective study. Serial 2-fold doubling dilutions were prepared for each sample to calculate the titer of anti-A and anti-B in a standard 96 well micro-plate. Tube technique was used for comparison with the microplate method for 100 samples. Results: Out of 1635 donors, 1430 (87.46%) were males and 205 (12.54%) were females. The median titer for anti-A and anti-B was 128 with range from 4 to 2048. Spearman's correlation coefficient for microplate versus tube technique was estimated to be 0.803 (P < 0.01, two-tailed). 57.12% and 51.19% of all donors had titers ≥128 for anti-A and anti-B, respectively. The geometric mean of anti-A and anti-B was 155.7 and 137.28, respectively. The titers were significantly higher (P < 0.001) in female donors. An inverse relation between titer levels and age was seen. Conclusion: Microplate can be used to perform titers in resource-constrained settings. Screening for critical titers in O group donors is essential as they are more implicated in hemolytic transfusion reactions. In the absence of a global consensus on this topic, institutes may need to formulate their own guidelines on handling ABO plasma-incompatible PLT transfusions.http://www.ajts.org/article.asp?issn=0973-6247;year=2017;volume=11;issue=1;spage=22;epage=27;aulast=TendulkarAntibody titershemolytic transfusion reactionO groupplatelet donor |
| spellingShingle | Anita Amar Tendulkar Puneet Ashok Jain Sanjay Velaye Antibody titers in Group O platelet donors Asian Journal of Transfusion Science Antibody titers hemolytic transfusion reaction O group platelet donor |
| title | Antibody titers in Group O platelet donors |
| title_full | Antibody titers in Group O platelet donors |
| title_fullStr | Antibody titers in Group O platelet donors |
| title_full_unstemmed | Antibody titers in Group O platelet donors |
| title_short | Antibody titers in Group O platelet donors |
| title_sort | antibody titers in group o platelet donors |
| topic | Antibody titers hemolytic transfusion reaction O group platelet donor |
| url | http://www.ajts.org/article.asp?issn=0973-6247;year=2017;volume=11;issue=1;spage=22;epage=27;aulast=Tendulkar |
| work_keys_str_mv | AT anitaamartendulkar antibodytitersingroupoplateletdonors AT puneetashokjain antibodytitersingroupoplateletdonors AT sanjayvelaye antibodytitersingroupoplateletdonors |