CRISPR/Cas9‐enhanced ssDNA recombineering for Pseudomonas putida
Summary Implementation of single‐stranded DNA (ssDNA) recombineering in Pseudomonas putida has widened the range of genetic manipulations applicable to this biotechnologically relevant bacterium. Yet, the relatively low efficiency of the technology hampers identification of mutated clones lacking co...
Main Authors: | Tomás Aparicio, Víctor deLorenzo, Esteban Martínez‐García |
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Format: | Article |
Language: | English |
Published: |
Wiley
2019-09-01
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Series: | Microbial Biotechnology |
Online Access: | https://doi.org/10.1111/1751-7915.13453 |
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