CRISPR/Cas9‐enhanced ssDNA recombineering for Pseudomonas putida

Summary Implementation of single‐stranded DNA (ssDNA) recombineering in Pseudomonas putida has widened the range of genetic manipulations applicable to this biotechnologically relevant bacterium. Yet, the relatively low efficiency of the technology hampers identification of mutated clones lacking co...

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Main Authors: Tomás Aparicio, Víctor deLorenzo, Esteban Martínez‐García
格式: 文件
语言:English
出版: Wiley 2019-09-01
丛编:Microbial Biotechnology
在线阅读:https://doi.org/10.1111/1751-7915.13453