Hydrogel Fiber Cultivation Method for Forming Bacterial Cellulose Microspheres
Forming microspheres or microbeads from nanofibrous materials has recently attracted research interest for their applications in various fields, because these structures greatly impact cellular behaviors and functions. However, conventional methods of preparing microspheres or microbeads have limita...
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| Format: | Article |
| Language: | English |
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MDPI AG
2018-01-01
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| Series: | Micromachines |
| Subjects: | |
| Online Access: | http://www.mdpi.com/2072-666X/9/1/36 |
| _version_ | 1819144161684619264 |
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| author | Kazuhiko Higashi Norihisa Miki |
| author_facet | Kazuhiko Higashi Norihisa Miki |
| author_sort | Kazuhiko Higashi |
| collection | DOAJ |
| description | Forming microspheres or microbeads from nanofibrous materials has recently attracted research interest for their applications in various fields, because these structures greatly impact cellular behaviors and functions. However, conventional methods of preparing microspheres or microbeads have limitations, such as limited variety of material. Here, we propose a new fabrication process for forming a nanofibrous microsphere composed of bacterial cellulose (BC), which is synthesized through fermentation by specific bacteria. The process uses a hydrogel fiber containing spherical cavities. The bacteria encapsulated into the cavities produce BC, resulting in the formation of BC microspheres. Because of its simplicity, robustness, and cost-effectiveness, this process is promising for applications, such as in biochemical engineering and cell delivery systems. |
| first_indexed | 2024-12-22T12:37:44Z |
| format | Article |
| id | doaj.art-bc30fa70716949b7ab4f5160a3141bb7 |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-12-22T12:37:44Z |
| publishDate | 2018-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-bc30fa70716949b7ab4f5160a3141bb72022-12-21T18:25:31ZengMDPI AGMicromachines2072-666X2018-01-01913610.3390/mi9010036mi9010036Hydrogel Fiber Cultivation Method for Forming Bacterial Cellulose MicrospheresKazuhiko Higashi0Norihisa Miki1School of Integrated Design Engineering, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Kanagawa, JapanSchool of Integrated Design Engineering, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Kanagawa, JapanForming microspheres or microbeads from nanofibrous materials has recently attracted research interest for their applications in various fields, because these structures greatly impact cellular behaviors and functions. However, conventional methods of preparing microspheres or microbeads have limitations, such as limited variety of material. Here, we propose a new fabrication process for forming a nanofibrous microsphere composed of bacterial cellulose (BC), which is synthesized through fermentation by specific bacteria. The process uses a hydrogel fiber containing spherical cavities. The bacteria encapsulated into the cavities produce BC, resulting in the formation of BC microspheres. Because of its simplicity, robustness, and cost-effectiveness, this process is promising for applications, such as in biochemical engineering and cell delivery systems.http://www.mdpi.com/2072-666X/9/1/36microbeadsmicrocarriersmicrobesmicrofabricationmicrofluidicsdropletemulsionmonodispersity |
| spellingShingle | Kazuhiko Higashi Norihisa Miki Hydrogel Fiber Cultivation Method for Forming Bacterial Cellulose Microspheres Micromachines microbeads microcarriers microbes microfabrication microfluidics droplet emulsion monodispersity |
| title | Hydrogel Fiber Cultivation Method for Forming Bacterial Cellulose Microspheres |
| title_full | Hydrogel Fiber Cultivation Method for Forming Bacterial Cellulose Microspheres |
| title_fullStr | Hydrogel Fiber Cultivation Method for Forming Bacterial Cellulose Microspheres |
| title_full_unstemmed | Hydrogel Fiber Cultivation Method for Forming Bacterial Cellulose Microspheres |
| title_short | Hydrogel Fiber Cultivation Method for Forming Bacterial Cellulose Microspheres |
| title_sort | hydrogel fiber cultivation method for forming bacterial cellulose microspheres |
| topic | microbeads microcarriers microbes microfabrication microfluidics droplet emulsion monodispersity |
| url | http://www.mdpi.com/2072-666X/9/1/36 |
| work_keys_str_mv | AT kazuhikohigashi hydrogelfibercultivationmethodforformingbacterialcellulosemicrospheres AT norihisamiki hydrogelfibercultivationmethodforformingbacterialcellulosemicrospheres |