Examination of the effect of xenogeneic mesenchymal stem cells and conditioned medium on cartilage graft viability: a rabbit model
Background Since cartilage, unlike skin, does not contain vessels, it obtains nutrition by diffusion. This reduces graft viability, resulting in problems such as reductions in size, changes in shape, and resorption of the cartilage graft in the late post-graft period. This study aimed to investigate...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Korean Society for Aesthetic Plastic Surgery
2023-07-01
|
| Series: | Archives of Aesthetic Plastic Surgery |
| Subjects: | |
| Online Access: | http://e-aaps.org/upload/pdf/aaps-2022-00773.pdf |
| _version_ | 1797755762171183104 |
|---|---|
| author | Abdullah Unlu Muhammet Volkan Bulbul Bircan Kolbasi Erkan İlkay Ozdemir Ilknur Keskin Mustafa Sutcu |
| author_facet | Abdullah Unlu Muhammet Volkan Bulbul Bircan Kolbasi Erkan İlkay Ozdemir Ilknur Keskin Mustafa Sutcu |
| author_sort | Abdullah Unlu |
| collection | DOAJ |
| description | Background Since cartilage, unlike skin, does not contain vessels, it obtains nutrition by diffusion. This reduces graft viability, resulting in problems such as reductions in size, changes in shape, and resorption of the cartilage graft in the late post-graft period. This study aimed to investigate the effects of adipose-derived mesenchymal cells and conditioned medium (CM) on cartilage graft viability. Methods Dissections were performed 4 months after the injection of 0.5 mL of CM or 2×106 mesenchymal stem cells (MSCs) in 0.5 mL after grafting into a control group and two experimental groups (n=21 rabbits in total). Chondrocyte viability and type II collagen expression in the grafted areas were analyzed by hematoxylin-eosin staining and immunohistochemical methods, respectively. Results In the MSC and CM groups, chondrocyte proliferation at the graft tissue incision margin (MSC: P<0.01, CM: P<0.0001), chondrocyte proliferation at the auricular cartilage incision margin (MSC: P<0.05, CM: P<0.0001), integration of the graft with the surrounding cartilage (MSC: P<0.001, CM: P<0.0001) and type II collagen expression levels (MSC: P=0.001, CM: P=0.0002) significantly increased. Conclusions Xenogenic injection of MSCs and CM contributed to new cartilage production without any tumoral effects or immune reactions. In particular, the cell-free nature of CM strengthened its potential for safe use. Since injections of MSC and CM can preserve cartilage graft viability, interest in this technique is expected to increase as long-term results from clinical studies on the subject become available. |
| first_indexed | 2024-03-12T17:51:52Z |
| format | Article |
| id | doaj.art-bc330f54d00342299d81c19de5c94ae0 |
| institution | Directory Open Access Journal |
| issn | 2234-0831 2288-9337 |
| language | English |
| last_indexed | 2024-03-12T17:51:52Z |
| publishDate | 2023-07-01 |
| publisher | Korean Society for Aesthetic Plastic Surgery |
| record_format | Article |
| series | Archives of Aesthetic Plastic Surgery |
| spelling | doaj.art-bc330f54d00342299d81c19de5c94ae02023-08-03T06:11:18ZengKorean Society for Aesthetic Plastic SurgeryArchives of Aesthetic Plastic Surgery2234-08312288-93372023-07-0129312913510.14730/aaps.2022.00773808Examination of the effect of xenogeneic mesenchymal stem cells and conditioned medium on cartilage graft viability: a rabbit modelAbdullah Unlu0Muhammet Volkan Bulbul1Bircan Kolbasi Erkan2İlkay Ozdemir3Ilknur Keskin4Mustafa Sutcu5 Department of Plastic Reconstructive and Aesthetic Surgery, Faculty of Medicine, Malatya Training and Research Hospital, Malatya, Turkey Department of Histology and Embryology, Faculty of Medicine, Nisantasi University, Istanbul, Turkey Department of Histology and Embryology, School of Medicine, Istanbul Medipol University, Istanbul, Turkey Department of Histology and Embryology, School of Medicine, Istanbul Medipol University, Istanbul, Turkey Department of Histology and Embryology, School of Medicine, Istanbul Medipol University, Istanbul, Turkey Department of Plastic Reconstructive and Aesthetic Surgery, Faculty of Medicine, Selcuk University, Konya, TurkeyBackground Since cartilage, unlike skin, does not contain vessels, it obtains nutrition by diffusion. This reduces graft viability, resulting in problems such as reductions in size, changes in shape, and resorption of the cartilage graft in the late post-graft period. This study aimed to investigate the effects of adipose-derived mesenchymal cells and conditioned medium (CM) on cartilage graft viability. Methods Dissections were performed 4 months after the injection of 0.5 mL of CM or 2×106 mesenchymal stem cells (MSCs) in 0.5 mL after grafting into a control group and two experimental groups (n=21 rabbits in total). Chondrocyte viability and type II collagen expression in the grafted areas were analyzed by hematoxylin-eosin staining and immunohistochemical methods, respectively. Results In the MSC and CM groups, chondrocyte proliferation at the graft tissue incision margin (MSC: P<0.01, CM: P<0.0001), chondrocyte proliferation at the auricular cartilage incision margin (MSC: P<0.05, CM: P<0.0001), integration of the graft with the surrounding cartilage (MSC: P<0.001, CM: P<0.0001) and type II collagen expression levels (MSC: P=0.001, CM: P=0.0002) significantly increased. Conclusions Xenogenic injection of MSCs and CM contributed to new cartilage production without any tumoral effects or immune reactions. In particular, the cell-free nature of CM strengthened its potential for safe use. Since injections of MSC and CM can preserve cartilage graft viability, interest in this technique is expected to increase as long-term results from clinical studies on the subject become available.http://e-aaps.org/upload/pdf/aaps-2022-00773.pdfcartilageadipose-derived mesenchymal stem cellsgraftcollagen type 2 |
| spellingShingle | Abdullah Unlu Muhammet Volkan Bulbul Bircan Kolbasi Erkan İlkay Ozdemir Ilknur Keskin Mustafa Sutcu Examination of the effect of xenogeneic mesenchymal stem cells and conditioned medium on cartilage graft viability: a rabbit model Archives of Aesthetic Plastic Surgery cartilage adipose-derived mesenchymal stem cells graft collagen type 2 |
| title | Examination of the effect of xenogeneic mesenchymal stem cells and conditioned medium on cartilage graft viability: a rabbit model |
| title_full | Examination of the effect of xenogeneic mesenchymal stem cells and conditioned medium on cartilage graft viability: a rabbit model |
| title_fullStr | Examination of the effect of xenogeneic mesenchymal stem cells and conditioned medium on cartilage graft viability: a rabbit model |
| title_full_unstemmed | Examination of the effect of xenogeneic mesenchymal stem cells and conditioned medium on cartilage graft viability: a rabbit model |
| title_short | Examination of the effect of xenogeneic mesenchymal stem cells and conditioned medium on cartilage graft viability: a rabbit model |
| title_sort | examination of the effect of xenogeneic mesenchymal stem cells and conditioned medium on cartilage graft viability a rabbit model |
| topic | cartilage adipose-derived mesenchymal stem cells graft collagen type 2 |
| url | http://e-aaps.org/upload/pdf/aaps-2022-00773.pdf |
| work_keys_str_mv | AT abdullahunlu examinationoftheeffectofxenogeneicmesenchymalstemcellsandconditionedmediumoncartilagegraftviabilityarabbitmodel AT muhammetvolkanbulbul examinationoftheeffectofxenogeneicmesenchymalstemcellsandconditionedmediumoncartilagegraftviabilityarabbitmodel AT bircankolbasierkan examinationoftheeffectofxenogeneicmesenchymalstemcellsandconditionedmediumoncartilagegraftviabilityarabbitmodel AT ilkayozdemir examinationoftheeffectofxenogeneicmesenchymalstemcellsandconditionedmediumoncartilagegraftviabilityarabbitmodel AT ilknurkeskin examinationoftheeffectofxenogeneicmesenchymalstemcellsandconditionedmediumoncartilagegraftviabilityarabbitmodel AT mustafasutcu examinationoftheeffectofxenogeneicmesenchymalstemcellsandconditionedmediumoncartilagegraftviabilityarabbitmodel |