Inflammatory related plasma proteins involved in acute preschool wheeze
Abstract Background Preschool wheeze is a risk factor for asthma development. However, the molecular mechanism behind a wheezing episode is not well understood. Objective Our aims were to assess the association of plasma proteins with acute preschool wheeze and to study the proteins with differentia...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2023-11-01
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| Series: | Clinical and Translational Allergy |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/clt2.12308 |
| _version_ | 1797454515597737984 |
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| author | Idun Holmdahl Sandip Chakraborty Angela Hoyer Anastasia Filiou Anna Asarnoj Anders Sjölander Magnus P. Borres Marianne vanHage Gunilla Hedlin Jon R. Konradsen Cilla Söderhäll |
| author_facet | Idun Holmdahl Sandip Chakraborty Angela Hoyer Anastasia Filiou Anna Asarnoj Anders Sjölander Magnus P. Borres Marianne vanHage Gunilla Hedlin Jon R. Konradsen Cilla Söderhäll |
| author_sort | Idun Holmdahl |
| collection | DOAJ |
| description | Abstract Background Preschool wheeze is a risk factor for asthma development. However, the molecular mechanism behind a wheezing episode is not well understood. Objective Our aims were to assess the association of plasma proteins with acute preschool wheeze and to study the proteins with differential expression at the acute phase at revisit after 3 months. Additionally, to investigate the relationship between protein expression and clinical parameters. Method We measured 92 inflammatory proteins in plasma and clinical parameters from 145 children during an episode of preschool wheeze (PW) and at the revisit after 3 months (PW‐R, n = 113/145) and 101 healthy controls (HC) aged 6–48 months in the GEWAC cohort using the antibody‐mediated proximity extension‐based assay (Olink Proteomics, Uppsala). Results Of the 74 analysed proteins, 52 were differentially expressed between PW and HC. The expression profiles of the top 10 proteins, Oncostatin M (OSM), IL‐10, IL‐6, Fibroblast growth factor 21 (FGF21), AXIN1, CXCL10, SIRT2, TNFSF11, Tumour necrosis factor β (TNF‐β) and CASP8, could almost entirely separate PW from HC. Five out of 10 proteins were associated with intake of oral corticosteroids (OCS) 24 h preceding blood sampling (OSM, CASP8, IL‐10, TNF‐β and CXCL10). No differences in protein expression were seen between PWs with or without OCS in comparison to HC. At the revisit after 3 months, differential protein expressions were still seen between PW‐R and HC for three (IL‐10, SIRT2 and FGF21) of the 10 proteins. Conclusion Our results contribute to unravelling potential immunopathological pathways shared between preschool wheeze and asthma. |
| first_indexed | 2024-03-09T15:38:20Z |
| format | Article |
| id | doaj.art-bc3312c44fd24cf493eaf83d91828089 |
| institution | Directory Open Access Journal |
| issn | 2045-7022 |
| language | English |
| last_indexed | 2024-03-09T15:38:20Z |
| publishDate | 2023-11-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Translational Allergy |
| spelling | doaj.art-bc3312c44fd24cf493eaf83d918280892023-11-25T14:38:27ZengWileyClinical and Translational Allergy2045-70222023-11-011311n/an/a10.1002/clt2.12308Inflammatory related plasma proteins involved in acute preschool wheezeIdun Holmdahl0Sandip Chakraborty1Angela Hoyer2Anastasia Filiou3Anna Asarnoj4Anders Sjölander5Magnus P. Borres6Marianne vanHage7Gunilla Hedlin8Jon R. Konradsen9Cilla Söderhäll10Department of Women's and Children's Health Karolinska Institutet Stockholm SwedenDepartment of Women's and Children's Health Karolinska Institutet Stockholm SwedenDepartment of Women's and Children's Health Karolinska Institutet Stockholm SwedenDepartment of Women's and Children's Health Karolinska Institutet Stockholm SwedenDepartment of Women's and Children's Health Karolinska Institutet Stockholm SwedenThermo Fisher Scientific Uppsala SwedenThermo Fisher Scientific Uppsala SwedenDivision of Immunology and Allergy Department of Medicine Solna Karolinska Institutet and Karolinska University Hospital Stockholm SwedenDepartment of Women's and Children's Health Karolinska Institutet Stockholm SwedenDepartment of Women's and Children's Health Karolinska Institutet Stockholm SwedenDepartment of Women's and Children's Health Karolinska Institutet Stockholm SwedenAbstract Background Preschool wheeze is a risk factor for asthma development. However, the molecular mechanism behind a wheezing episode is not well understood. Objective Our aims were to assess the association of plasma proteins with acute preschool wheeze and to study the proteins with differential expression at the acute phase at revisit after 3 months. Additionally, to investigate the relationship between protein expression and clinical parameters. Method We measured 92 inflammatory proteins in plasma and clinical parameters from 145 children during an episode of preschool wheeze (PW) and at the revisit after 3 months (PW‐R, n = 113/145) and 101 healthy controls (HC) aged 6–48 months in the GEWAC cohort using the antibody‐mediated proximity extension‐based assay (Olink Proteomics, Uppsala). Results Of the 74 analysed proteins, 52 were differentially expressed between PW and HC. The expression profiles of the top 10 proteins, Oncostatin M (OSM), IL‐10, IL‐6, Fibroblast growth factor 21 (FGF21), AXIN1, CXCL10, SIRT2, TNFSF11, Tumour necrosis factor β (TNF‐β) and CASP8, could almost entirely separate PW from HC. Five out of 10 proteins were associated with intake of oral corticosteroids (OCS) 24 h preceding blood sampling (OSM, CASP8, IL‐10, TNF‐β and CXCL10). No differences in protein expression were seen between PWs with or without OCS in comparison to HC. At the revisit after 3 months, differential protein expressions were still seen between PW‐R and HC for three (IL‐10, SIRT2 and FGF21) of the 10 proteins. Conclusion Our results contribute to unravelling potential immunopathological pathways shared between preschool wheeze and asthma.https://doi.org/10.1002/clt2.12308asthmainflammationolinkplasma proteinspreschool wheeze |
| spellingShingle | Idun Holmdahl Sandip Chakraborty Angela Hoyer Anastasia Filiou Anna Asarnoj Anders Sjölander Magnus P. Borres Marianne vanHage Gunilla Hedlin Jon R. Konradsen Cilla Söderhäll Inflammatory related plasma proteins involved in acute preschool wheeze Clinical and Translational Allergy asthma inflammation olink plasma proteins preschool wheeze |
| title | Inflammatory related plasma proteins involved in acute preschool wheeze |
| title_full | Inflammatory related plasma proteins involved in acute preschool wheeze |
| title_fullStr | Inflammatory related plasma proteins involved in acute preschool wheeze |
| title_full_unstemmed | Inflammatory related plasma proteins involved in acute preschool wheeze |
| title_short | Inflammatory related plasma proteins involved in acute preschool wheeze |
| title_sort | inflammatory related plasma proteins involved in acute preschool wheeze |
| topic | asthma inflammation olink plasma proteins preschool wheeze |
| url | https://doi.org/10.1002/clt2.12308 |
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