Quantitative determination of 0.05 % chlorhexidine solution by capillary electrophoresis
Abstract The aim of the work was to estimate the quantitative content of 0.05 % chlorhexidine solution by capillary electrophoresis. Materials and methods. The object of the study was the drug 0.05 % solution of chlorhexidine for local and external use of pharmacopoeia quality. The study was car...
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| Format: | Article |
| Language: | English |
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Zaporozhye State Medical University
2019-07-01
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| Series: | Zaporožskij Medicinskij Žurnal |
| Subjects: | |
| Online Access: | http://zmj.zsmu.edu.ua/article/view/173352/173883 |
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| author | A. M. Sampiiev N. A. Davitavian Ye. B. Nikiforova Yu. F. Yakuba |
| author_facet | A. M. Sampiiev N. A. Davitavian Ye. B. Nikiforova Yu. F. Yakuba |
| author_sort | A. M. Sampiiev |
| collection | DOAJ |
| description | Abstract
The aim of the work was to estimate the quantitative content of 0.05 % chlorhexidine solution by capillary electrophoresis.
Materials and methods. The object of the study was the drug 0.05 % solution of chlorhexidine for local and external use of pharmacopoeia quality. The study was carried out on a Kapel-104T device, equipped with an ultraviolet photometric detector and personal computer with the Multichrom software.
Electrophoretic determination of the quantitative content of chlorhexidine was carried out in the UV region of the spectrum at a wavelength of 254 nm with a positive voltage on a capillary of 16 kV and a recommended capillary thermostating from 20 °C to 30 °C. The sample was introduced in pneumatical mode in the 30 mbar for 5 seconds. The analysis time was 5 minutes. As the leading electrolyte an aqueous solution consisting of 0.6 % solution of imidazole, 0.6 % solution of sodium tetraborate and 0.5 % solution of tartaric acid were used. The determination of chlorhexidine in the test solution was carried out in the UV spectral region at a wavelength of 254 nm, since at this wavelength one of the absorption maxima of the indicated drug was observed.
Results. The validation analysis showed that the technique is characterized by specificity, linearity in the concentration range from 100 μg/ml to 700 μg/ml (y = 0.3438х - 12.4789; r = 0.9913), accuracy for chlorhexidine levels of 80–120 % (R = 98.82–100.44 %), precision (Sr = 0.44–0.61 %). The relative error of the result of separate determination of chlorhexidine in 0.05 % solution using capillary electrophoresis was 1.28 %.
Conclusions. Quantitative determination of 0.05 % chlorhexidine solution by capillary electrophoresis was carried out and the parameters of the validation of the electrophoretic technique were established. The proposed electrophoretic conditions can serve as the basis for the development of methods for the quantitative determination of chlorhexidine bigluconate as part of this drug. |
| first_indexed | 2024-12-11T11:07:10Z |
| format | Article |
| id | doaj.art-bc334b6081194ce69b00faabb3b63223 |
| institution | Directory Open Access Journal |
| issn | 2306-4145 2310-1210 |
| language | English |
| last_indexed | 2024-12-11T11:07:10Z |
| publishDate | 2019-07-01 |
| publisher | Zaporozhye State Medical University |
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| series | Zaporožskij Medicinskij Žurnal |
| spelling | doaj.art-bc334b6081194ce69b00faabb3b632232022-12-22T01:09:40ZengZaporozhye State Medical UniversityZaporožskij Medicinskij Žurnal2306-41452310-12102019-07-0121451752110.14739/2310-1210.2019.4.173352Quantitative determination of 0.05 % chlorhexidine solution by capillary electrophoresisA. M. SampiievN. A. DavitavianYe. B. NikiforovaYu. F. YakubaAbstract The aim of the work was to estimate the quantitative content of 0.05 % chlorhexidine solution by capillary electrophoresis. Materials and methods. The object of the study was the drug 0.05 % solution of chlorhexidine for local and external use of pharmacopoeia quality. The study was carried out on a Kapel-104T device, equipped with an ultraviolet photometric detector and personal computer with the Multichrom software. Electrophoretic determination of the quantitative content of chlorhexidine was carried out in the UV region of the spectrum at a wavelength of 254 nm with a positive voltage on a capillary of 16 kV and a recommended capillary thermostating from 20 °C to 30 °C. The sample was introduced in pneumatical mode in the 30 mbar for 5 seconds. The analysis time was 5 minutes. As the leading electrolyte an aqueous solution consisting of 0.6 % solution of imidazole, 0.6 % solution of sodium tetraborate and 0.5 % solution of tartaric acid were used. The determination of chlorhexidine in the test solution was carried out in the UV spectral region at a wavelength of 254 nm, since at this wavelength one of the absorption maxima of the indicated drug was observed. Results. The validation analysis showed that the technique is characterized by specificity, linearity in the concentration range from 100 μg/ml to 700 μg/ml (y = 0.3438х - 12.4789; r = 0.9913), accuracy for chlorhexidine levels of 80–120 % (R = 98.82–100.44 %), precision (Sr = 0.44–0.61 %). The relative error of the result of separate determination of chlorhexidine in 0.05 % solution using capillary electrophoresis was 1.28 %. Conclusions. Quantitative determination of 0.05 % chlorhexidine solution by capillary electrophoresis was carried out and the parameters of the validation of the electrophoretic technique were established. The proposed electrophoretic conditions can serve as the basis for the development of methods for the quantitative determination of chlorhexidine bigluconate as part of this drug.http://zmj.zsmu.edu.ua/article/view/173352/173883chlorhexidinecapillary zone electrophoresisbiguanidesamidinesantiseptics |
| spellingShingle | A. M. Sampiiev N. A. Davitavian Ye. B. Nikiforova Yu. F. Yakuba Quantitative determination of 0.05 % chlorhexidine solution by capillary electrophoresis Zaporožskij Medicinskij Žurnal chlorhexidine capillary zone electrophoresis biguanides amidines antiseptics |
| title | Quantitative determination of 0.05 % chlorhexidine solution by capillary electrophoresis |
| title_full | Quantitative determination of 0.05 % chlorhexidine solution by capillary electrophoresis |
| title_fullStr | Quantitative determination of 0.05 % chlorhexidine solution by capillary electrophoresis |
| title_full_unstemmed | Quantitative determination of 0.05 % chlorhexidine solution by capillary electrophoresis |
| title_short | Quantitative determination of 0.05 % chlorhexidine solution by capillary electrophoresis |
| title_sort | quantitative determination of 0 05 chlorhexidine solution by capillary electrophoresis |
| topic | chlorhexidine capillary zone electrophoresis biguanides amidines antiseptics |
| url | http://zmj.zsmu.edu.ua/article/view/173352/173883 |
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