Microscale tissue engineering of liver lobule models: advancements and applications
The liver, as the body’s primary organ for maintaining internal balance, is composed of numerous hexagonal liver lobules, each sharing a uniform architectural framework. These liver lobules serve as the basic structural and functional units of the liver, comprised of central veins, hepatic plates, h...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-12-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1303053/full |
| _version_ | 1797400610468790272 |
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| author | Qi Wang Juan Liu Juan Liu Juan Liu Juan Liu Wenzhen Yin Anqi Wang Jingjing Zheng Yunfang Wang Yunfang Wang Yunfang Wang Yunfang Wang Yunfang Wang Jiahong Dong Jiahong Dong Jiahong Dong Jiahong Dong Jiahong Dong |
| author_facet | Qi Wang Juan Liu Juan Liu Juan Liu Juan Liu Wenzhen Yin Anqi Wang Jingjing Zheng Yunfang Wang Yunfang Wang Yunfang Wang Yunfang Wang Yunfang Wang Jiahong Dong Jiahong Dong Jiahong Dong Jiahong Dong Jiahong Dong |
| author_sort | Qi Wang |
| collection | DOAJ |
| description | The liver, as the body’s primary organ for maintaining internal balance, is composed of numerous hexagonal liver lobules, each sharing a uniform architectural framework. These liver lobules serve as the basic structural and functional units of the liver, comprised of central veins, hepatic plates, hepatic sinusoids, and minute bile ducts. Meanwhile, within liver lobules, distinct regions of hepatocytes carry out diverse functions. The in vitro construction of liver lobule models, faithfully replicating their structure and function, holds paramount significance for research in liver development and diseases. Presently, two primary technologies for constructing liver lobule models dominate the field: 3D bioprinting and microfluidic techniques. 3D bioprinting enables precise deposition of cells and biomaterials, while microfluidics facilitates targeted transport of cells or other culture materials to specified locations, effectively managing culture media input and output through micro-pump control, enabling dynamic simulations of liver lobules. In this comprehensive review, we provide an overview of the biomaterials, cells, and manufacturing methods employed by recent researchers in constructing liver lobule models. Our aim is to explore strategies and technologies that closely emulate the authentic structure and function of liver lobules, offering invaluable insights for research into liver diseases, drug screening, drug toxicity assessment, and cell replacement therapy. |
| first_indexed | 2024-03-09T01:58:01Z |
| format | Article |
| id | doaj.art-bc33c92fc9c74a6e98ff078af2e3ed4c |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-03-09T01:58:01Z |
| publishDate | 2023-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-bc33c92fc9c74a6e98ff078af2e3ed4c2023-12-08T12:32:47ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852023-12-011110.3389/fbioe.2023.13030531303053Microscale tissue engineering of liver lobule models: advancements and applicationsQi Wang0Juan Liu1Juan Liu2Juan Liu3Juan Liu4Wenzhen Yin5Anqi Wang6Jingjing Zheng7Yunfang Wang8Yunfang Wang9Yunfang Wang10Yunfang Wang11Yunfang Wang12Jiahong Dong13Jiahong Dong14Jiahong Dong15Jiahong Dong16Jiahong Dong17Department of Hepatobiliary and Pancreatic Surgery, The First Hospital of Jilin University, Jilin University, Changchun, ChinaResearch Unit of Precision Hepatobiliary Surgery Paradigm, Chinese Academy of Medical Sciences, Beijing, ChinaHepato-Pancreato-Biliary Center, Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Tsinghua University, Beijing, ChinaInstitute for Organ Transplant and Bionic Medicine, Tsinghua University, Beijing, ChinaKey Laboratory of Digital Intelligence Hepatology, Ministry of Education, School of Clinical Medicine, Tsinghua University, Beijing, ChinaClinical Translational Science Center, Beijing Tsinghua Changgung Hospital, Tsinghua University, Beijing, ChinaHepato-Pancreato-Biliary Center, Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Tsinghua University, Beijing, ChinaHepato-Pancreato-Biliary Center, Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Tsinghua University, Beijing, ChinaResearch Unit of Precision Hepatobiliary Surgery Paradigm, Chinese Academy of Medical Sciences, Beijing, ChinaHepato-Pancreato-Biliary Center, Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Tsinghua University, Beijing, ChinaInstitute for Organ Transplant and Bionic Medicine, Tsinghua University, Beijing, ChinaKey Laboratory of Digital Intelligence Hepatology, Ministry of Education, School of Clinical Medicine, Tsinghua University, Beijing, ChinaClinical Translational Science Center, Beijing Tsinghua Changgung Hospital, Tsinghua University, Beijing, ChinaDepartment of Hepatobiliary and Pancreatic Surgery, The First Hospital of Jilin University, Jilin University, Changchun, ChinaResearch Unit of Precision Hepatobiliary Surgery Paradigm, Chinese Academy of Medical Sciences, Beijing, ChinaHepato-Pancreato-Biliary Center, Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Tsinghua University, Beijing, ChinaInstitute for Organ Transplant and Bionic Medicine, Tsinghua University, Beijing, ChinaKey Laboratory of Digital Intelligence Hepatology, Ministry of Education, School of Clinical Medicine, Tsinghua University, Beijing, ChinaThe liver, as the body’s primary organ for maintaining internal balance, is composed of numerous hexagonal liver lobules, each sharing a uniform architectural framework. These liver lobules serve as the basic structural and functional units of the liver, comprised of central veins, hepatic plates, hepatic sinusoids, and minute bile ducts. Meanwhile, within liver lobules, distinct regions of hepatocytes carry out diverse functions. The in vitro construction of liver lobule models, faithfully replicating their structure and function, holds paramount significance for research in liver development and diseases. Presently, two primary technologies for constructing liver lobule models dominate the field: 3D bioprinting and microfluidic techniques. 3D bioprinting enables precise deposition of cells and biomaterials, while microfluidics facilitates targeted transport of cells or other culture materials to specified locations, effectively managing culture media input and output through micro-pump control, enabling dynamic simulations of liver lobules. In this comprehensive review, we provide an overview of the biomaterials, cells, and manufacturing methods employed by recent researchers in constructing liver lobule models. Our aim is to explore strategies and technologies that closely emulate the authentic structure and function of liver lobules, offering invaluable insights for research into liver diseases, drug screening, drug toxicity assessment, and cell replacement therapy.https://www.frontiersin.org/articles/10.3389/fbioe.2023.1303053/fullliver lobule model3D bioprintingmicrofluidicsbiomaterialsbiomimic |
| spellingShingle | Qi Wang Juan Liu Juan Liu Juan Liu Juan Liu Wenzhen Yin Anqi Wang Jingjing Zheng Yunfang Wang Yunfang Wang Yunfang Wang Yunfang Wang Yunfang Wang Jiahong Dong Jiahong Dong Jiahong Dong Jiahong Dong Jiahong Dong Microscale tissue engineering of liver lobule models: advancements and applications Frontiers in Bioengineering and Biotechnology liver lobule model 3D bioprinting microfluidics biomaterials biomimic |
| title | Microscale tissue engineering of liver lobule models: advancements and applications |
| title_full | Microscale tissue engineering of liver lobule models: advancements and applications |
| title_fullStr | Microscale tissue engineering of liver lobule models: advancements and applications |
| title_full_unstemmed | Microscale tissue engineering of liver lobule models: advancements and applications |
| title_short | Microscale tissue engineering of liver lobule models: advancements and applications |
| title_sort | microscale tissue engineering of liver lobule models advancements and applications |
| topic | liver lobule model 3D bioprinting microfluidics biomaterials biomimic |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1303053/full |
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