Intravital imaging of immune responses in intestinal inflammation
Abstract To date, many kinds of immune cells have been identified, but their precise roles in intestinal immunity remain unclear. Understanding the in vivo behavior of these immune cells and their function in gastrointestinal inflammation, including colitis, inflammatory bowel disease, ischemia–repe...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2023-02-01
|
| Series: | Inflammation and Regeneration |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s41232-023-00262-w |
| _version_ | 1811171842462842880 |
|---|---|
| author | Masaki Honda Masashi Kadohisa Daiki Yoshii Yoshihiro Komohara Taizo Hibi |
| author_facet | Masaki Honda Masashi Kadohisa Daiki Yoshii Yoshihiro Komohara Taizo Hibi |
| author_sort | Masaki Honda |
| collection | DOAJ |
| description | Abstract To date, many kinds of immune cells have been identified, but their precise roles in intestinal immunity remain unclear. Understanding the in vivo behavior of these immune cells and their function in gastrointestinal inflammation, including colitis, inflammatory bowel disease, ischemia–reperfusion injury, and neutrophil extracellular traps, is critical for gastrointestinal research to proceed to the next step. Additionally, understanding the immune responses involved in gastrointestinal tumors and tissue repair is becoming increasingly important for the elucidation of disease mechanisms that have been unknown. In recent years, the application of intravital microscopy in gastrointestinal research has provided novel insights into the mechanisms of intestine-specific events including innate and adaptive immunities. In this review, we focus on the emerging role of intravital imaging in gastrointestinal research and describe how to observe the intestines and immune cells using intravital microscopy. Additionally, we outline novel findings obtained by this new technique. |
| first_indexed | 2024-04-10T17:20:54Z |
| format | Article |
| id | doaj.art-bc36f736f9d04dbcae4a464206f3ded1 |
| institution | Directory Open Access Journal |
| issn | 1880-8190 |
| language | English |
| last_indexed | 2024-04-10T17:20:54Z |
| publishDate | 2023-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Inflammation and Regeneration |
| spelling | doaj.art-bc36f736f9d04dbcae4a464206f3ded12023-02-05T12:06:53ZengBMCInflammation and Regeneration1880-81902023-02-014311910.1186/s41232-023-00262-wIntravital imaging of immune responses in intestinal inflammationMasaki Honda0Masashi Kadohisa1Daiki Yoshii2Yoshihiro Komohara3Taizo Hibi4Department of Pediatric Surgery and Transplantation, Kumamoto University Graduate School of Medical SciencesDepartment of Pediatric Surgery and Transplantation, Kumamoto University Graduate School of Medical SciencesDepartment of Diagnostic Pathology, Kumamoto University HospitalDepartment of Cell Pathology, Kumamoto University Graduate School of Medical SciencesDepartment of Pediatric Surgery and Transplantation, Kumamoto University Graduate School of Medical SciencesAbstract To date, many kinds of immune cells have been identified, but their precise roles in intestinal immunity remain unclear. Understanding the in vivo behavior of these immune cells and their function in gastrointestinal inflammation, including colitis, inflammatory bowel disease, ischemia–reperfusion injury, and neutrophil extracellular traps, is critical for gastrointestinal research to proceed to the next step. Additionally, understanding the immune responses involved in gastrointestinal tumors and tissue repair is becoming increasingly important for the elucidation of disease mechanisms that have been unknown. In recent years, the application of intravital microscopy in gastrointestinal research has provided novel insights into the mechanisms of intestine-specific events including innate and adaptive immunities. In this review, we focus on the emerging role of intravital imaging in gastrointestinal research and describe how to observe the intestines and immune cells using intravital microscopy. Additionally, we outline novel findings obtained by this new technique.https://doi.org/10.1186/s41232-023-00262-wIntravital imagingImmune cellsIntestinal inflammationColorectal cancerTissue repair |
| spellingShingle | Masaki Honda Masashi Kadohisa Daiki Yoshii Yoshihiro Komohara Taizo Hibi Intravital imaging of immune responses in intestinal inflammation Inflammation and Regeneration Intravital imaging Immune cells Intestinal inflammation Colorectal cancer Tissue repair |
| title | Intravital imaging of immune responses in intestinal inflammation |
| title_full | Intravital imaging of immune responses in intestinal inflammation |
| title_fullStr | Intravital imaging of immune responses in intestinal inflammation |
| title_full_unstemmed | Intravital imaging of immune responses in intestinal inflammation |
| title_short | Intravital imaging of immune responses in intestinal inflammation |
| title_sort | intravital imaging of immune responses in intestinal inflammation |
| topic | Intravital imaging Immune cells Intestinal inflammation Colorectal cancer Tissue repair |
| url | https://doi.org/10.1186/s41232-023-00262-w |
| work_keys_str_mv | AT masakihonda intravitalimagingofimmuneresponsesinintestinalinflammation AT masashikadohisa intravitalimagingofimmuneresponsesinintestinalinflammation AT daikiyoshii intravitalimagingofimmuneresponsesinintestinalinflammation AT yoshihirokomohara intravitalimagingofimmuneresponsesinintestinalinflammation AT taizohibi intravitalimagingofimmuneresponsesinintestinalinflammation |