Association between miRNA-145 and miRNA-155 expression in peripheral blood mononuclear cells of patients with multiple sclerosis: a case-control study
Abstract Introduction MicroRNAs (miR or miRNA) are short regulatory RNAs, which modulate post-transcriptional gene expression. Dysregulation of these molecules contributes to pathogenicity of autoimmune disorders, such as multiple sclerosis (MS). Aims This study was conducted to investigate changed...
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| Format: | Article |
| Language: | English |
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BMC
2022-11-01
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| Series: | BMC Neurology |
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| Online Access: | https://doi.org/10.1186/s12883-022-02909-6 |
| _version_ | 1811328741160255488 |
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| author | Sepide Ali Ashrafi Milad Asadi Dariush Shanehbandi Saeed Sadigh Eteghad Asra Fazlollahi Seyed Aria Nejadghaderi Sheida Shaafi |
| author_facet | Sepide Ali Ashrafi Milad Asadi Dariush Shanehbandi Saeed Sadigh Eteghad Asra Fazlollahi Seyed Aria Nejadghaderi Sheida Shaafi |
| author_sort | Sepide Ali Ashrafi |
| collection | DOAJ |
| description | Abstract Introduction MicroRNAs (miR or miRNA) are short regulatory RNAs, which modulate post-transcriptional gene expression. Dysregulation of these molecules contributes to pathogenicity of autoimmune disorders, such as multiple sclerosis (MS). Aims This study was conducted to investigate changed expression pattern of miRNA-145 and miRNA-155 in MS. Methods We collected blood samples of 75 patients with relapsing-remitting MS patients and 75 healthy controls. Ficoll-Hypaque density gradient method was used to isolate peripheral blood mononuclear cells. Also, total RNA was extracted and subjected to RT-PCR analysis. We used the Mann–Whitney U test to evaluate the differences in expression levels of target miRNAs between the groups. Results We found that expression of miRNA-145 (P = 0.012) and miRNA-155 (P = 0.005) were partly reduced in patients with relapse-remitting MS in comparison with healthy controls. The miRNA-145 had an area under curve (AUC) of 0.621 (P = 0.01) and miRNA-155 levels had an AUC of 0.625 (P = 0.008). Conclusion Decreased expression of miRNA-145 and miRNA-155 contributes to development of relapse-remitting MS, while further large scale observational studies and meta-analyses are required. |
| first_indexed | 2024-04-13T15:31:20Z |
| format | Article |
| id | doaj.art-bc38e317dc48412f8d5072ed29d046b6 |
| institution | Directory Open Access Journal |
| issn | 1471-2377 |
| language | English |
| last_indexed | 2024-04-13T15:31:20Z |
| publishDate | 2022-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Neurology |
| spelling | doaj.art-bc38e317dc48412f8d5072ed29d046b62022-12-22T02:41:23ZengBMCBMC Neurology1471-23772022-11-012211510.1186/s12883-022-02909-6Association between miRNA-145 and miRNA-155 expression in peripheral blood mononuclear cells of patients with multiple sclerosis: a case-control studySepide Ali Ashrafi0Milad Asadi1Dariush Shanehbandi2Saeed Sadigh Eteghad3Asra Fazlollahi4Seyed Aria Nejadghaderi5Sheida Shaafi6Neurosciences Research Center (NSRC), Tabriz University of Medical SciencesDepartment of Basic Oncology, Health Institute of Ege UniversityImmunology Research Center, Tabriz University of Medical SciencesNeurosciences Research Center (NSRC), Tabriz University of Medical SciencesStudent Research Committee, Tabriz University of Medical SciencesResearch Center for Integrative Medicine in Aging, Aging Research Institute, Tabriz University of Medical SciencesNeurosciences Research Center (NSRC), Tabriz University of Medical SciencesAbstract Introduction MicroRNAs (miR or miRNA) are short regulatory RNAs, which modulate post-transcriptional gene expression. Dysregulation of these molecules contributes to pathogenicity of autoimmune disorders, such as multiple sclerosis (MS). Aims This study was conducted to investigate changed expression pattern of miRNA-145 and miRNA-155 in MS. Methods We collected blood samples of 75 patients with relapsing-remitting MS patients and 75 healthy controls. Ficoll-Hypaque density gradient method was used to isolate peripheral blood mononuclear cells. Also, total RNA was extracted and subjected to RT-PCR analysis. We used the Mann–Whitney U test to evaluate the differences in expression levels of target miRNAs between the groups. Results We found that expression of miRNA-145 (P = 0.012) and miRNA-155 (P = 0.005) were partly reduced in patients with relapse-remitting MS in comparison with healthy controls. The miRNA-145 had an area under curve (AUC) of 0.621 (P = 0.01) and miRNA-155 levels had an AUC of 0.625 (P = 0.008). Conclusion Decreased expression of miRNA-145 and miRNA-155 contributes to development of relapse-remitting MS, while further large scale observational studies and meta-analyses are required.https://doi.org/10.1186/s12883-022-02909-6microRNAmiR-145miR-155Real-time PCRRelapsing-remitting MSMultiple sclerosis |
| spellingShingle | Sepide Ali Ashrafi Milad Asadi Dariush Shanehbandi Saeed Sadigh Eteghad Asra Fazlollahi Seyed Aria Nejadghaderi Sheida Shaafi Association between miRNA-145 and miRNA-155 expression in peripheral blood mononuclear cells of patients with multiple sclerosis: a case-control study BMC Neurology microRNA miR-145 miR-155 Real-time PCR Relapsing-remitting MS Multiple sclerosis |
| title | Association between miRNA-145 and miRNA-155 expression in peripheral blood mononuclear cells of patients with multiple sclerosis: a case-control study |
| title_full | Association between miRNA-145 and miRNA-155 expression in peripheral blood mononuclear cells of patients with multiple sclerosis: a case-control study |
| title_fullStr | Association between miRNA-145 and miRNA-155 expression in peripheral blood mononuclear cells of patients with multiple sclerosis: a case-control study |
| title_full_unstemmed | Association between miRNA-145 and miRNA-155 expression in peripheral blood mononuclear cells of patients with multiple sclerosis: a case-control study |
| title_short | Association between miRNA-145 and miRNA-155 expression in peripheral blood mononuclear cells of patients with multiple sclerosis: a case-control study |
| title_sort | association between mirna 145 and mirna 155 expression in peripheral blood mononuclear cells of patients with multiple sclerosis a case control study |
| topic | microRNA miR-145 miR-155 Real-time PCR Relapsing-remitting MS Multiple sclerosis |
| url | https://doi.org/10.1186/s12883-022-02909-6 |
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