Glucansucrase Produced by Lactic Acid Bacteria: Structure, Properties, and Applications
Glucansucrase (GS) belongs to the GH70 family, which not only can synthesize exopolysaccharides (EPSs) with different physicochemical properties through glucosyl transglycosylation (by hydrolyzing sucrose) but can also produce oligosaccharides. Different strains produce different GSs, which catalyze...
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MDPI AG
2022-11-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/8/11/629 |
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| author | Liansheng Yu Zhigang Qian Jingping Ge Renpeng Du |
| author_facet | Liansheng Yu Zhigang Qian Jingping Ge Renpeng Du |
| author_sort | Liansheng Yu |
| collection | DOAJ |
| description | Glucansucrase (GS) belongs to the GH70 family, which not only can synthesize exopolysaccharides (EPSs) with different physicochemical properties through glucosyl transglycosylation (by hydrolyzing sucrose) but can also produce oligosaccharides. Different strains produce different GSs, which catalyze the synthesis of EPS with different glycosidic bond structures; these EPSs have different biological functions. As an important enzymatic tool, GS has great potential in health care medicine, biological materials, ecological protection, the food chemical industry, etc. GS is mainly produced by lactic acid bacteria (LAB), including <i>Leuconostoc</i>, <i>Streptococcus</i>, <i>Lactobacillus</i>, and <i>Weissella</i> species. With the elucidation of the crystal structure of GS and the advancement of genome sequencing technology, its synthesis reaction mechanism and specific structural characteristics are gradually becoming clear. This review summarizes the isolation, purification, physical and chemical properties, detection methods, sources, and applications of GS in order to provide a reference for the research and development of GS. |
| first_indexed | 2024-03-09T18:20:56Z |
| format | Article |
| id | doaj.art-bc38e55e3c174a5fbf7be87517787bd4 |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-09T18:20:56Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-bc38e55e3c174a5fbf7be87517787bd42023-11-24T08:19:35ZengMDPI AGFermentation2311-56372022-11-0181162910.3390/fermentation8110629Glucansucrase Produced by Lactic Acid Bacteria: Structure, Properties, and ApplicationsLiansheng Yu0Zhigang Qian1Jingping Ge2Renpeng Du3Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education & Heilongjiang Provincial Key Laboratory of Plant Genetic Engineering and Biological Fermentation Engineering for Cold Region & Key Laboratory of Microbiology, College of Heilongjiang Province & School of Life Sciences, Heilongjiang University, Harbin 150080, ChinaState Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, ChinaEngineering Research Center of Agricultural Microbiology Technology, Ministry of Education & Heilongjiang Provincial Key Laboratory of Plant Genetic Engineering and Biological Fermentation Engineering for Cold Region & Key Laboratory of Microbiology, College of Heilongjiang Province & School of Life Sciences, Heilongjiang University, Harbin 150080, ChinaEngineering Research Center of Agricultural Microbiology Technology, Ministry of Education & Heilongjiang Provincial Key Laboratory of Plant Genetic Engineering and Biological Fermentation Engineering for Cold Region & Key Laboratory of Microbiology, College of Heilongjiang Province & School of Life Sciences, Heilongjiang University, Harbin 150080, ChinaGlucansucrase (GS) belongs to the GH70 family, which not only can synthesize exopolysaccharides (EPSs) with different physicochemical properties through glucosyl transglycosylation (by hydrolyzing sucrose) but can also produce oligosaccharides. Different strains produce different GSs, which catalyze the synthesis of EPS with different glycosidic bond structures; these EPSs have different biological functions. As an important enzymatic tool, GS has great potential in health care medicine, biological materials, ecological protection, the food chemical industry, etc. GS is mainly produced by lactic acid bacteria (LAB), including <i>Leuconostoc</i>, <i>Streptococcus</i>, <i>Lactobacillus</i>, and <i>Weissella</i> species. With the elucidation of the crystal structure of GS and the advancement of genome sequencing technology, its synthesis reaction mechanism and specific structural characteristics are gradually becoming clear. This review summarizes the isolation, purification, physical and chemical properties, detection methods, sources, and applications of GS in order to provide a reference for the research and development of GS.https://www.mdpi.com/2311-5637/8/11/629lactic acid bacteriaglucansucraseexopolysaccharidesstructure and propertiesapplications |
| spellingShingle | Liansheng Yu Zhigang Qian Jingping Ge Renpeng Du Glucansucrase Produced by Lactic Acid Bacteria: Structure, Properties, and Applications Fermentation lactic acid bacteria glucansucrase exopolysaccharides structure and properties applications |
| title | Glucansucrase Produced by Lactic Acid Bacteria: Structure, Properties, and Applications |
| title_full | Glucansucrase Produced by Lactic Acid Bacteria: Structure, Properties, and Applications |
| title_fullStr | Glucansucrase Produced by Lactic Acid Bacteria: Structure, Properties, and Applications |
| title_full_unstemmed | Glucansucrase Produced by Lactic Acid Bacteria: Structure, Properties, and Applications |
| title_short | Glucansucrase Produced by Lactic Acid Bacteria: Structure, Properties, and Applications |
| title_sort | glucansucrase produced by lactic acid bacteria structure properties and applications |
| topic | lactic acid bacteria glucansucrase exopolysaccharides structure and properties applications |
| url | https://www.mdpi.com/2311-5637/8/11/629 |
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