Easy and quick (EQ) sperm freezing method for urgent preservation of mouse strains
Abstract Cryopreservation of mouse spermatozoa is widely used for the efficient preservation and safe transport of valuable mouse strains. However, the current cryopreservation method requires special containers (plastic straws), undefined chemicals (e.g., skim milk), liquid nitrogen, and expertise...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2021-07-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-93604-y |
| _version_ | 1819202667700813824 |
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| author | Keiji Mochida Ayumi Hasegawa Daiki Shikata Nobuhiko Itami Masashi Hada Naomi Watanabe Toshiko Tomishima Atsuo Ogura |
| author_facet | Keiji Mochida Ayumi Hasegawa Daiki Shikata Nobuhiko Itami Masashi Hada Naomi Watanabe Toshiko Tomishima Atsuo Ogura |
| author_sort | Keiji Mochida |
| collection | DOAJ |
| description | Abstract Cryopreservation of mouse spermatozoa is widely used for the efficient preservation and safe transport of valuable mouse strains. However, the current cryopreservation method requires special containers (plastic straws), undefined chemicals (e.g., skim milk), liquid nitrogen, and expertise when handling sperm suspensions. Here, we report an easy and quick (EQ) sperm freezing method. The main procedure consists of only one step: dissecting a single cauda epididymis in a microtube containing 20% raffinose solution, which is then stored in a −80 °C freezer. The frozen–thawed spermatozoa retain practical fertilization rates after 1 (51%) or even 3 months (25%) with the C57BL/6 J strain, the most sensitive strain for sperm freezing. More than half of the embryos thus obtained developed into offspring after embryo transfer. Importantly, spermatozoa stored at −80 °C can be transferred into liquid nitrogen for indefinite storage. As far as we know, our EQ method is the easiest and quickest method for mouse sperm freezing and should be applicable in all laboratories without expertise in sperm cryopreservation. This technique can help avoid the loss of irreplaceable strains because of closure of animal rooms in emergency situations such as unexpected microbiological contamination or social emergencies such as the COVID-19 threat. |
| first_indexed | 2024-12-23T04:07:40Z |
| format | Article |
| id | doaj.art-bc39811397874fd4bcdd714e9d6a1b3d |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-23T04:07:40Z |
| publishDate | 2021-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-bc39811397874fd4bcdd714e9d6a1b3d2022-12-21T18:00:35ZengNature PortfolioScientific Reports2045-23222021-07-0111111210.1038/s41598-021-93604-yEasy and quick (EQ) sperm freezing method for urgent preservation of mouse strainsKeiji Mochida0Ayumi Hasegawa1Daiki Shikata2Nobuhiko Itami3Masashi Hada4Naomi Watanabe5Toshiko Tomishima6Atsuo Ogura7RIKEN BioResouce Research CenterRIKEN BioResouce Research CenterRIKEN BioResouce Research CenterRIKEN BioResouce Research CenterRIKEN BioResouce Research CenterRIKEN BioResouce Research CenterRIKEN BioResouce Research CenterRIKEN BioResouce Research CenterAbstract Cryopreservation of mouse spermatozoa is widely used for the efficient preservation and safe transport of valuable mouse strains. However, the current cryopreservation method requires special containers (plastic straws), undefined chemicals (e.g., skim milk), liquid nitrogen, and expertise when handling sperm suspensions. Here, we report an easy and quick (EQ) sperm freezing method. The main procedure consists of only one step: dissecting a single cauda epididymis in a microtube containing 20% raffinose solution, which is then stored in a −80 °C freezer. The frozen–thawed spermatozoa retain practical fertilization rates after 1 (51%) or even 3 months (25%) with the C57BL/6 J strain, the most sensitive strain for sperm freezing. More than half of the embryos thus obtained developed into offspring after embryo transfer. Importantly, spermatozoa stored at −80 °C can be transferred into liquid nitrogen for indefinite storage. As far as we know, our EQ method is the easiest and quickest method for mouse sperm freezing and should be applicable in all laboratories without expertise in sperm cryopreservation. This technique can help avoid the loss of irreplaceable strains because of closure of animal rooms in emergency situations such as unexpected microbiological contamination or social emergencies such as the COVID-19 threat.https://doi.org/10.1038/s41598-021-93604-y |
| spellingShingle | Keiji Mochida Ayumi Hasegawa Daiki Shikata Nobuhiko Itami Masashi Hada Naomi Watanabe Toshiko Tomishima Atsuo Ogura Easy and quick (EQ) sperm freezing method for urgent preservation of mouse strains Scientific Reports |
| title | Easy and quick (EQ) sperm freezing method for urgent preservation of mouse strains |
| title_full | Easy and quick (EQ) sperm freezing method for urgent preservation of mouse strains |
| title_fullStr | Easy and quick (EQ) sperm freezing method for urgent preservation of mouse strains |
| title_full_unstemmed | Easy and quick (EQ) sperm freezing method for urgent preservation of mouse strains |
| title_short | Easy and quick (EQ) sperm freezing method for urgent preservation of mouse strains |
| title_sort | easy and quick eq sperm freezing method for urgent preservation of mouse strains |
| url | https://doi.org/10.1038/s41598-021-93604-y |
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