A microplate technique to simultaneously assay calcium accumulation in endoplasmic reticulum and SERCA release of inorganic phosphate
<p>Abstract</p> <p>Traditional analyses of calcium homeostasis have separately quantified either calcium accumulation or release mechanisms. To define the system as a whole, however, requires multiple experimental techniques to examine both accumulation and release. Here we describ...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2012-04-01
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| Series: | Biological Procedures Online |
| Subjects: | |
| Online Access: | http://www.biologicalproceduresonline.com/content/14/1/4 |
| _version_ | 1818581779534053376 |
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| author | McMullen David C Kean William S Verma Ajay Cole Jeffrey T Watson William D |
| author_facet | McMullen David C Kean William S Verma Ajay Cole Jeffrey T Watson William D |
| author_sort | McMullen David C |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Traditional analyses of calcium homeostasis have separately quantified either calcium accumulation or release mechanisms. To define the system as a whole, however, requires multiple experimental techniques to examine both accumulation and release. Here we describe a technique that couples the simultaneous quantification of radio-labeled calcium accumulation in endoplasmic reticulum (ER) microsomes with the release of inorganic phosphate (Pi) by the hydrolytic activity of sarco-endoplasmic reticulum calcium ATPase (SERCA) all in the convenience of a 96-well format.</p> |
| first_indexed | 2024-12-16T07:38:55Z |
| format | Article |
| id | doaj.art-bc3ac7882b49486ea6b8706a47a92248 |
| institution | Directory Open Access Journal |
| issn | 1480-9222 |
| language | English |
| last_indexed | 2024-12-16T07:38:55Z |
| publishDate | 2012-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Biological Procedures Online |
| spelling | doaj.art-bc3ac7882b49486ea6b8706a47a922482022-12-21T22:39:08ZengBMCBiological Procedures Online1480-92222012-04-01141410.1186/1480-9222-14-4A microplate technique to simultaneously assay calcium accumulation in endoplasmic reticulum and SERCA release of inorganic phosphateMcMullen David CKean William SVerma AjayCole Jeffrey TWatson William D<p>Abstract</p> <p>Traditional analyses of calcium homeostasis have separately quantified either calcium accumulation or release mechanisms. To define the system as a whole, however, requires multiple experimental techniques to examine both accumulation and release. Here we describe a technique that couples the simultaneous quantification of radio-labeled calcium accumulation in endoplasmic reticulum (ER) microsomes with the release of inorganic phosphate (Pi) by the hydrolytic activity of sarco-endoplasmic reticulum calcium ATPase (SERCA) all in the convenience of a 96-well format.</p>http://www.biologicalproceduresonline.com/content/14/1/4CalciumSERCA activityMicrosomesInorganic phosphateMalachite green |
| spellingShingle | McMullen David C Kean William S Verma Ajay Cole Jeffrey T Watson William D A microplate technique to simultaneously assay calcium accumulation in endoplasmic reticulum and SERCA release of inorganic phosphate Biological Procedures Online Calcium SERCA activity Microsomes Inorganic phosphate Malachite green |
| title | A microplate technique to simultaneously assay calcium accumulation in endoplasmic reticulum and SERCA release of inorganic phosphate |
| title_full | A microplate technique to simultaneously assay calcium accumulation in endoplasmic reticulum and SERCA release of inorganic phosphate |
| title_fullStr | A microplate technique to simultaneously assay calcium accumulation in endoplasmic reticulum and SERCA release of inorganic phosphate |
| title_full_unstemmed | A microplate technique to simultaneously assay calcium accumulation in endoplasmic reticulum and SERCA release of inorganic phosphate |
| title_short | A microplate technique to simultaneously assay calcium accumulation in endoplasmic reticulum and SERCA release of inorganic phosphate |
| title_sort | microplate technique to simultaneously assay calcium accumulation in endoplasmic reticulum and serca release of inorganic phosphate |
| topic | Calcium SERCA activity Microsomes Inorganic phosphate Malachite green |
| url | http://www.biologicalproceduresonline.com/content/14/1/4 |
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