The Relationship between Seminal Melatonin with Sperm Parameters, DNA Fragmentation and Nuclear Maturity in Intra-Cytoplasmic Sperm Injection Candidates
Objective: Melatonin, the chief secretory product of the pineal gland, regulates dynamic physiological adaptations that occur in seasonally breeding mammals as a response to changes in daylight hours. Because of the presence of melatonin in semen and the membrane melatonin receptor in spermatozoa...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Royan Institute (ACECR), Tehran
2015-10-01
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| Series: | Cell Journal |
| Subjects: | |
| Online Access: | http://celljournal.org/web/journal/article/1018/download |
| _version_ | 1819002846055497728 |
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| author | Mina Sharbatoghli Mojtaba Rezazadeh Valojerdi Mohammad Hadi Bahadori Reza Salman Yazdi Leila Rashki Ghaleno |
| author_facet | Mina Sharbatoghli Mojtaba Rezazadeh Valojerdi Mohammad Hadi Bahadori Reza Salman Yazdi Leila Rashki Ghaleno |
| author_sort | Mina Sharbatoghli |
| collection | DOAJ |
| description | Objective: Melatonin, the chief secretory product of the pineal gland, regulates dynamic
physiological adaptations that occur in seasonally breeding mammals as a response to
changes in daylight hours. Because of the presence of melatonin in semen and the membrane
melatonin receptor in spermatozoa, the impact of melatonin on the regulation of
male infertility is still questionable. The aim of this study was to determine the effects of
endogenous melatonin on human semen parameters (sperm concentration, motility and
normal morphology), DNA fragmentation (DF) and nuclear maturity.
Materials and Methods: In this clinical prospective study, semen samples from 75 infertile
men were routinely analyzed and assessed for melatonin and total antioxidant capacity
(TAC) levels using the enzyme-linked immunosorbent assay (ELISA) and colorimetric
assay kits, respectively. DF was examined by the sperm chromatin dispersion (SCD) test.
Acidic aniline blue staining was used to detect chromatin defects in the sperm nuclei.
Results: There was no significant correlation between seminal plasma melatonin and
TAC with sperm parameters and nuclear maturity. However, we observed a positive
significant correlation between DF and melatonin level (r=0.273, P<0.05).
Conclusion: Melatonin in seminal plasma is positively correlated with damaged sperm
DNA of infertile patients. The mechanism of this phenomenon needs further study. |
| first_indexed | 2024-12-20T23:11:35Z |
| format | Article |
| id | doaj.art-bc3ba955a26443a9a019add8896336d4 |
| institution | Directory Open Access Journal |
| issn | 2228-5806 2228-5814 |
| language | English |
| last_indexed | 2024-12-20T23:11:35Z |
| publishDate | 2015-10-01 |
| publisher | Royan Institute (ACECR), Tehran |
| record_format | Article |
| series | Cell Journal |
| spelling | doaj.art-bc3ba955a26443a9a019add8896336d42022-12-21T19:23:44ZengRoyan Institute (ACECR), TehranCell Journal2228-58062228-58142015-10-01173547553The Relationship between Seminal Melatonin with Sperm Parameters, DNA Fragmentation and Nuclear Maturity in Intra-Cytoplasmic Sperm Injection CandidatesMina Sharbatoghli0Mojtaba Rezazadeh Valojerdi1Mohammad Hadi Bahadori2Reza Salman Yazdi3Leila Rashki Ghaleno4Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, IranDepartment of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, IranDepartment of Anatomy, Faculty of Medical Science, Guilan University of Medical Sciences, Rasht, IranDepartment of Andrology, Reproductive Biomedicine Rersearch Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, IranDepartment of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, IranObjective: Melatonin, the chief secretory product of the pineal gland, regulates dynamic physiological adaptations that occur in seasonally breeding mammals as a response to changes in daylight hours. Because of the presence of melatonin in semen and the membrane melatonin receptor in spermatozoa, the impact of melatonin on the regulation of male infertility is still questionable. The aim of this study was to determine the effects of endogenous melatonin on human semen parameters (sperm concentration, motility and normal morphology), DNA fragmentation (DF) and nuclear maturity. Materials and Methods: In this clinical prospective study, semen samples from 75 infertile men were routinely analyzed and assessed for melatonin and total antioxidant capacity (TAC) levels using the enzyme-linked immunosorbent assay (ELISA) and colorimetric assay kits, respectively. DF was examined by the sperm chromatin dispersion (SCD) test. Acidic aniline blue staining was used to detect chromatin defects in the sperm nuclei. Results: There was no significant correlation between seminal plasma melatonin and TAC with sperm parameters and nuclear maturity. However, we observed a positive significant correlation between DF and melatonin level (r=0.273, P<0.05). Conclusion: Melatonin in seminal plasma is positively correlated with damaged sperm DNA of infertile patients. The mechanism of this phenomenon needs further study.http://celljournal.org/web/journal/article/1018/downloadMelatoninDNA FragmentationAntioxidantSperm Maturation |
| spellingShingle | Mina Sharbatoghli Mojtaba Rezazadeh Valojerdi Mohammad Hadi Bahadori Reza Salman Yazdi Leila Rashki Ghaleno The Relationship between Seminal Melatonin with Sperm Parameters, DNA Fragmentation and Nuclear Maturity in Intra-Cytoplasmic Sperm Injection Candidates Cell Journal Melatonin DNA Fragmentation Antioxidant Sperm Maturation |
| title | The Relationship between Seminal Melatonin with Sperm Parameters, DNA Fragmentation and Nuclear Maturity in Intra-Cytoplasmic Sperm Injection Candidates |
| title_full | The Relationship between Seminal Melatonin with Sperm Parameters, DNA Fragmentation and Nuclear Maturity in Intra-Cytoplasmic Sperm Injection Candidates |
| title_fullStr | The Relationship between Seminal Melatonin with Sperm Parameters, DNA Fragmentation and Nuclear Maturity in Intra-Cytoplasmic Sperm Injection Candidates |
| title_full_unstemmed | The Relationship between Seminal Melatonin with Sperm Parameters, DNA Fragmentation and Nuclear Maturity in Intra-Cytoplasmic Sperm Injection Candidates |
| title_short | The Relationship between Seminal Melatonin with Sperm Parameters, DNA Fragmentation and Nuclear Maturity in Intra-Cytoplasmic Sperm Injection Candidates |
| title_sort | relationship between seminal melatonin with sperm parameters dna fragmentation and nuclear maturity in intra cytoplasmic sperm injection candidates |
| topic | Melatonin DNA Fragmentation Antioxidant Sperm Maturation |
| url | http://celljournal.org/web/journal/article/1018/download |
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