Integrated nontargeted and targeted metabolomics analyses amino acids metabolism in infantile hemangioma
Infantile hemangioma (IH) is the most common benign tumor in children. However, the exact pathogenesis of IH remains unclear. Integrated nontargeted and targeted metabolic analyses were performed to obtain insight into the possible pathogenic mechanism of IH. The results of nontargeted metabolic ana...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-03-01
|
| Series: | Frontiers in Oncology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fonc.2023.1132344/full |
| _version_ | 1797866862109786112 |
|---|---|
| author | Kaiying Yang Kaiying Yang Tong Qiu Xue Gong Jiangyuan Zhou Yuru Lan Siyuan Chen Yi Ji |
| author_facet | Kaiying Yang Kaiying Yang Tong Qiu Xue Gong Jiangyuan Zhou Yuru Lan Siyuan Chen Yi Ji |
| author_sort | Kaiying Yang |
| collection | DOAJ |
| description | Infantile hemangioma (IH) is the most common benign tumor in children. However, the exact pathogenesis of IH remains unclear. Integrated nontargeted and targeted metabolic analyses were performed to obtain insight into the possible pathogenic mechanism of IH. The results of nontargeted metabolic analysis showed that 216 and 128 differential metabolites (DMs) were identified between hemangioma-derived endothelial cells (HemECs) and HUVECs in positive-ion and negative-ion models, respectively. In both models, these DMs were predominantly enriched in pathways related to amino acid metabolism, including aminoacyl-tRNA biosynthesis and arginine and proline metabolism. Then, targeted metabolic analysis of amino acids was further performed to further clarify HemEC metabolism. A total of 22 amino acid metabolites were identified, among which only 16 metabolites, including glutamine, arginine and asparagine, were significantly differentially expressed between HemECs and HUVECs. These significant amino acids were significantly enriched in 10 metabolic pathways, including ‘alanine, aspartate and glutamate metabolism’, ‘arginine biosynthesis’, ‘arginine and proline metabolism’, and ‘glycine, serine and threonine metabolism’. The results of our study revealed that amino acid metabolism is involved in IH. Key differential amino acid metabolites, including glutamine, asparagine and arginine, may play an important role in regulating HemEC metabolism. |
| first_indexed | 2024-04-09T23:31:08Z |
| format | Article |
| id | doaj.art-bc3bb03e3def4152bf6aa3f26302ef03 |
| institution | Directory Open Access Journal |
| issn | 2234-943X |
| language | English |
| last_indexed | 2024-04-09T23:31:08Z |
| publishDate | 2023-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-bc3bb03e3def4152bf6aa3f26302ef032023-03-21T05:21:25ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2023-03-011310.3389/fonc.2023.11323441132344Integrated nontargeted and targeted metabolomics analyses amino acids metabolism in infantile hemangiomaKaiying Yang0Kaiying Yang1Tong Qiu2Xue Gong3Jiangyuan Zhou4Yuru Lan5Siyuan Chen6Yi Ji7Division of Oncology, Department of Pediatric Surgery, West China Hospital of Sichuan University, Chengdu, Sichuan, ChinaDepartment of Pediatric Surgery, Guangzhou Women and Children’s Medical Center, National Children’s Medical Center for South Central Region, Guangzhou Medical University, Guangzhou, ChinaDivision of Oncology, Department of Pediatric Surgery, West China Hospital of Sichuan University, Chengdu, Sichuan, ChinaDivision of Oncology, Department of Pediatric Surgery, West China Hospital of Sichuan University, Chengdu, Sichuan, ChinaDivision of Oncology, Department of Pediatric Surgery, West China Hospital of Sichuan University, Chengdu, Sichuan, ChinaDivision of Oncology, Department of Pediatric Surgery, West China Hospital of Sichuan University, Chengdu, Sichuan, ChinaPediatric Intensive Care Unit, Department of Critical Care Medicine, West China Hospital of Sichuan University, Chengdu, ChinaDivision of Oncology, Department of Pediatric Surgery, West China Hospital of Sichuan University, Chengdu, Sichuan, ChinaInfantile hemangioma (IH) is the most common benign tumor in children. However, the exact pathogenesis of IH remains unclear. Integrated nontargeted and targeted metabolic analyses were performed to obtain insight into the possible pathogenic mechanism of IH. The results of nontargeted metabolic analysis showed that 216 and 128 differential metabolites (DMs) were identified between hemangioma-derived endothelial cells (HemECs) and HUVECs in positive-ion and negative-ion models, respectively. In both models, these DMs were predominantly enriched in pathways related to amino acid metabolism, including aminoacyl-tRNA biosynthesis and arginine and proline metabolism. Then, targeted metabolic analysis of amino acids was further performed to further clarify HemEC metabolism. A total of 22 amino acid metabolites were identified, among which only 16 metabolites, including glutamine, arginine and asparagine, were significantly differentially expressed between HemECs and HUVECs. These significant amino acids were significantly enriched in 10 metabolic pathways, including ‘alanine, aspartate and glutamate metabolism’, ‘arginine biosynthesis’, ‘arginine and proline metabolism’, and ‘glycine, serine and threonine metabolism’. The results of our study revealed that amino acid metabolism is involved in IH. Key differential amino acid metabolites, including glutamine, asparagine and arginine, may play an important role in regulating HemEC metabolism.https://www.frontiersin.org/articles/10.3389/fonc.2023.1132344/fullinfantile hemangiomahemangioma-derived endothelial cellamino acidsnontargeted metabolictargeted metabolic |
| spellingShingle | Kaiying Yang Kaiying Yang Tong Qiu Xue Gong Jiangyuan Zhou Yuru Lan Siyuan Chen Yi Ji Integrated nontargeted and targeted metabolomics analyses amino acids metabolism in infantile hemangioma Frontiers in Oncology infantile hemangioma hemangioma-derived endothelial cell amino acids nontargeted metabolic targeted metabolic |
| title | Integrated nontargeted and targeted metabolomics analyses amino acids metabolism in infantile hemangioma |
| title_full | Integrated nontargeted and targeted metabolomics analyses amino acids metabolism in infantile hemangioma |
| title_fullStr | Integrated nontargeted and targeted metabolomics analyses amino acids metabolism in infantile hemangioma |
| title_full_unstemmed | Integrated nontargeted and targeted metabolomics analyses amino acids metabolism in infantile hemangioma |
| title_short | Integrated nontargeted and targeted metabolomics analyses amino acids metabolism in infantile hemangioma |
| title_sort | integrated nontargeted and targeted metabolomics analyses amino acids metabolism in infantile hemangioma |
| topic | infantile hemangioma hemangioma-derived endothelial cell amino acids nontargeted metabolic targeted metabolic |
| url | https://www.frontiersin.org/articles/10.3389/fonc.2023.1132344/full |
| work_keys_str_mv | AT kaiyingyang integratednontargetedandtargetedmetabolomicsanalysesaminoacidsmetabolismininfantilehemangioma AT kaiyingyang integratednontargetedandtargetedmetabolomicsanalysesaminoacidsmetabolismininfantilehemangioma AT tongqiu integratednontargetedandtargetedmetabolomicsanalysesaminoacidsmetabolismininfantilehemangioma AT xuegong integratednontargetedandtargetedmetabolomicsanalysesaminoacidsmetabolismininfantilehemangioma AT jiangyuanzhou integratednontargetedandtargetedmetabolomicsanalysesaminoacidsmetabolismininfantilehemangioma AT yurulan integratednontargetedandtargetedmetabolomicsanalysesaminoacidsmetabolismininfantilehemangioma AT siyuanchen integratednontargetedandtargetedmetabolomicsanalysesaminoacidsmetabolismininfantilehemangioma AT yiji integratednontargetedandtargetedmetabolomicsanalysesaminoacidsmetabolismininfantilehemangioma |