Characterization of AI-2/LuxS quorum sensing system in biofilm formation, pathogenesis of Streptococcus equi subsp. zooepidemicus
Streptococcus equi subsp. zooepidemicus (SEZ) is an opportunistic pathogen of both humans and animals. Quorum sensing (QS) plays an important role in the regulation of bacterial group behaviors. The aim of this study was to characterize the LuxS in SEZ and evaluate its impact on biofilm formation, p...
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Frontiers Media S.A.
2024-02-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2024.1339131/full |
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| author | Honglin Xie Riteng Zhang Ruhai Guo Yining Zhang Jingya Zhang Hui Li Qiang Fu Xinglong Wang |
| author_facet | Honglin Xie Riteng Zhang Ruhai Guo Yining Zhang Jingya Zhang Hui Li Qiang Fu Xinglong Wang |
| author_sort | Honglin Xie |
| collection | DOAJ |
| description | Streptococcus equi subsp. zooepidemicus (SEZ) is an opportunistic pathogen of both humans and animals. Quorum sensing (QS) plays an important role in the regulation of bacterial group behaviors. The aim of this study was to characterize the LuxS in SEZ and evaluate its impact on biofilm formation, pathogenesis and gene expression. The wild-type SEZ and its LuxS mutant (ΔluxS) were examined for growth, biofilm formation, virulence factors, and transcriptomic profiles. Our results showed that LuxS deficiency did not affect SEZ hemolytic activity, adhesion or capsule production. For biofilm assay demonstrated that mutation in the luxS gene significantly enhances biofilm formation, produced a denser biofilm and attached to a glass surface. RAW264.7 cell infection indicated that ΔluxS promoted macrophage apoptosis and pro-inflammatory responses. In mice infection, there was no significant difference in mortality between SEZ and ΔluxS. However, the bacterial load in the spleen of mice infected with ΔluxS was significantly higher than in those infected with SEZ. And the pathological analysis further indicated that spleen damage was more severe in the ΔluxS group. Moreover, transcriptomics analysis revealed significant alterations in carbon metabolism, RNA binding and stress response genes in ΔluxS. In summary, this study provides the first evidence of AI-2/LuxS QS system in SEZ and reveals its regulatory effects on biofilm formation, pathogenicity and gene expression. |
| first_indexed | 2024-03-08T05:32:20Z |
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| id | doaj.art-bc3c4cd243e547178c11d979bebfa5d9 |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-03-08T05:32:20Z |
| publishDate | 2024-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-bc3c4cd243e547178c11d979bebfa5d92024-02-06T04:37:14ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882024-02-011410.3389/fcimb.2024.13391311339131Characterization of AI-2/LuxS quorum sensing system in biofilm formation, pathogenesis of Streptococcus equi subsp. zooepidemicusHonglin Xie0Riteng Zhang1Ruhai Guo2Yining Zhang3Jingya Zhang4Hui Li5Qiang Fu6Xinglong Wang7College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, ChinaCollege of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, ChinaCollege of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, ChinaCollege of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, ChinaCollege of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, ChinaCollege of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, ChinaSchool of Life Science and Engineering, Foshan University, Foshan, ChinaCollege of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, ChinaStreptococcus equi subsp. zooepidemicus (SEZ) is an opportunistic pathogen of both humans and animals. Quorum sensing (QS) plays an important role in the regulation of bacterial group behaviors. The aim of this study was to characterize the LuxS in SEZ and evaluate its impact on biofilm formation, pathogenesis and gene expression. The wild-type SEZ and its LuxS mutant (ΔluxS) were examined for growth, biofilm formation, virulence factors, and transcriptomic profiles. Our results showed that LuxS deficiency did not affect SEZ hemolytic activity, adhesion or capsule production. For biofilm assay demonstrated that mutation in the luxS gene significantly enhances biofilm formation, produced a denser biofilm and attached to a glass surface. RAW264.7 cell infection indicated that ΔluxS promoted macrophage apoptosis and pro-inflammatory responses. In mice infection, there was no significant difference in mortality between SEZ and ΔluxS. However, the bacterial load in the spleen of mice infected with ΔluxS was significantly higher than in those infected with SEZ. And the pathological analysis further indicated that spleen damage was more severe in the ΔluxS group. Moreover, transcriptomics analysis revealed significant alterations in carbon metabolism, RNA binding and stress response genes in ΔluxS. In summary, this study provides the first evidence of AI-2/LuxS QS system in SEZ and reveals its regulatory effects on biofilm formation, pathogenicity and gene expression.https://www.frontiersin.org/articles/10.3389/fcimb.2024.1339131/fullStreptococcus equi subsp. zooepidemicusLuxSbiofilmcapsular polysaccharidevirulenceinfection |
| spellingShingle | Honglin Xie Riteng Zhang Ruhai Guo Yining Zhang Jingya Zhang Hui Li Qiang Fu Xinglong Wang Characterization of AI-2/LuxS quorum sensing system in biofilm formation, pathogenesis of Streptococcus equi subsp. zooepidemicus Frontiers in Cellular and Infection Microbiology Streptococcus equi subsp. zooepidemicus LuxS biofilm capsular polysaccharide virulence infection |
| title | Characterization of AI-2/LuxS quorum sensing system in biofilm formation, pathogenesis of Streptococcus equi subsp. zooepidemicus |
| title_full | Characterization of AI-2/LuxS quorum sensing system in biofilm formation, pathogenesis of Streptococcus equi subsp. zooepidemicus |
| title_fullStr | Characterization of AI-2/LuxS quorum sensing system in biofilm formation, pathogenesis of Streptococcus equi subsp. zooepidemicus |
| title_full_unstemmed | Characterization of AI-2/LuxS quorum sensing system in biofilm formation, pathogenesis of Streptococcus equi subsp. zooepidemicus |
| title_short | Characterization of AI-2/LuxS quorum sensing system in biofilm formation, pathogenesis of Streptococcus equi subsp. zooepidemicus |
| title_sort | characterization of ai 2 luxs quorum sensing system in biofilm formation pathogenesis of streptococcus equi subsp zooepidemicus |
| topic | Streptococcus equi subsp. zooepidemicus LuxS biofilm capsular polysaccharide virulence infection |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2024.1339131/full |
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