Systemic inflammatory proteins in offspring following maternal probiotic supplementation for atopic dermatitis prevention
Abstract Background Maternal probiotic supplementation has a promising effect on atopic dermatitis (AD) prevention in infancy. In the randomised controlled study, Probiotics in the Prevention of Allergy among Children in Trondheim (ProPACT), maternal probiotics reduced the cumulative incidence of AD...
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| Format: | Article |
| Language: | English |
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BMC
2023-07-01
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| Series: | Clinical and Molecular Allergy |
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| Online Access: | https://doi.org/10.1186/s12948-023-00186-3 |
| _version_ | 1797769389327515648 |
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| author | Dinastry Pramadita Zakiudin Anne Dorthea Bjerkenes Rø Vibeke Videm Torbjørn Øien Melanie Rae Simpson |
| author_facet | Dinastry Pramadita Zakiudin Anne Dorthea Bjerkenes Rø Vibeke Videm Torbjørn Øien Melanie Rae Simpson |
| author_sort | Dinastry Pramadita Zakiudin |
| collection | DOAJ |
| description | Abstract Background Maternal probiotic supplementation has a promising effect on atopic dermatitis (AD) prevention in infancy. In the randomised controlled study, Probiotics in the Prevention of Allergy among Children in Trondheim (ProPACT), maternal probiotics reduced the cumulative incidence of AD in their offspring by 40% at 2 years of age. However, our understanding on how probiotics prevented AD is still limited, and the role of inflammatory proteins in infants following maternal probiotic supplementation is unclear. We hypothesised that maternal probiotics lowered pro-inflammatory proteins and increased anti-inflammatory proteins in their 2-year-old children as a mechanism of AD prevention. We aimed to explore this hypothesis and the association between these proteins and the presence of AD, severity of AD, and the degree of preventive effect of probiotics. Methods Plasma samples were collected from 2-year-old children (n = 202) during the ProPACT study, a randomised placebo-controlled trial of maternal probiotic supplementation. These samples were analysed for 92 inflammatory proteins using a multiplex proximity extension assay. Associations between inflammatory proteins and the presence and severity of AD, and the degree of preventive effect, was estimated individually using regression analysis and then collectively using unsupervised cluster analysis. Results Several proteins were observed to differ between the groups. The probiotic group had lower CCL11 and IL-17C, while children with AD had higher IL-17C, MCP-4, uPA, and CD6. Cytokine CCL20 and IL-18 had moderate correlation (r = 0.35 and r = 0.46) with the severity of AD. The cluster analysis revealed that children in the cluster of samples with the highest value of immune checkpoint receptors and inflammatory suppressor enzymes showed the greatest AD preventive effect from probiotics. Conclusions The proteins associated with both maternal probiotic supplementation and the presence and severity of AD warrant attention because of their potential biological relevance. Cluster analysis may provide a new insight when considering which subgroups benefit from probiotic supplementation. Larger studies are needed to confirm the results. Trial registration number: The study was retrospectively registered at ClinicalTrials.gov (NCT00159523) on 12nd September 2005. |
| first_indexed | 2024-03-12T21:08:21Z |
| format | Article |
| id | doaj.art-bc3df511f29e4750b103586c58df617d |
| institution | Directory Open Access Journal |
| issn | 1476-7961 |
| language | English |
| last_indexed | 2024-03-12T21:08:21Z |
| publishDate | 2023-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Clinical and Molecular Allergy |
| spelling | doaj.art-bc3df511f29e4750b103586c58df617d2023-07-30T11:21:58ZengBMCClinical and Molecular Allergy1476-79612023-07-0121111110.1186/s12948-023-00186-3Systemic inflammatory proteins in offspring following maternal probiotic supplementation for atopic dermatitis preventionDinastry Pramadita Zakiudin0Anne Dorthea Bjerkenes Rø1Vibeke Videm2Torbjørn Øien3Melanie Rae Simpson4Department of Public Health and Nursing, NTNU, Norwegian University of Science and TechnologyDepartment of Clinical and Molecular Medicine, NTNU, Norwegian University of Science and TechnologyDepartment of Clinical and Molecular Medicine, NTNU, Norwegian University of Science and TechnologyDepartment of Public Health and Nursing, NTNU, Norwegian University of Science and TechnologyDepartment of Public Health and Nursing, NTNU, Norwegian University of Science and TechnologyAbstract Background Maternal probiotic supplementation has a promising effect on atopic dermatitis (AD) prevention in infancy. In the randomised controlled study, Probiotics in the Prevention of Allergy among Children in Trondheim (ProPACT), maternal probiotics reduced the cumulative incidence of AD in their offspring by 40% at 2 years of age. However, our understanding on how probiotics prevented AD is still limited, and the role of inflammatory proteins in infants following maternal probiotic supplementation is unclear. We hypothesised that maternal probiotics lowered pro-inflammatory proteins and increased anti-inflammatory proteins in their 2-year-old children as a mechanism of AD prevention. We aimed to explore this hypothesis and the association between these proteins and the presence of AD, severity of AD, and the degree of preventive effect of probiotics. Methods Plasma samples were collected from 2-year-old children (n = 202) during the ProPACT study, a randomised placebo-controlled trial of maternal probiotic supplementation. These samples were analysed for 92 inflammatory proteins using a multiplex proximity extension assay. Associations between inflammatory proteins and the presence and severity of AD, and the degree of preventive effect, was estimated individually using regression analysis and then collectively using unsupervised cluster analysis. Results Several proteins were observed to differ between the groups. The probiotic group had lower CCL11 and IL-17C, while children with AD had higher IL-17C, MCP-4, uPA, and CD6. Cytokine CCL20 and IL-18 had moderate correlation (r = 0.35 and r = 0.46) with the severity of AD. The cluster analysis revealed that children in the cluster of samples with the highest value of immune checkpoint receptors and inflammatory suppressor enzymes showed the greatest AD preventive effect from probiotics. Conclusions The proteins associated with both maternal probiotic supplementation and the presence and severity of AD warrant attention because of their potential biological relevance. Cluster analysis may provide a new insight when considering which subgroups benefit from probiotic supplementation. Larger studies are needed to confirm the results. Trial registration number: The study was retrospectively registered at ClinicalTrials.gov (NCT00159523) on 12nd September 2005.https://doi.org/10.1186/s12948-023-00186-3Atopic dermatitisBiomarkersChildrenPlasmaProbiotics |
| spellingShingle | Dinastry Pramadita Zakiudin Anne Dorthea Bjerkenes Rø Vibeke Videm Torbjørn Øien Melanie Rae Simpson Systemic inflammatory proteins in offspring following maternal probiotic supplementation for atopic dermatitis prevention Clinical and Molecular Allergy Atopic dermatitis Biomarkers Children Plasma Probiotics |
| title | Systemic inflammatory proteins in offspring following maternal probiotic supplementation for atopic dermatitis prevention |
| title_full | Systemic inflammatory proteins in offspring following maternal probiotic supplementation for atopic dermatitis prevention |
| title_fullStr | Systemic inflammatory proteins in offspring following maternal probiotic supplementation for atopic dermatitis prevention |
| title_full_unstemmed | Systemic inflammatory proteins in offspring following maternal probiotic supplementation for atopic dermatitis prevention |
| title_short | Systemic inflammatory proteins in offspring following maternal probiotic supplementation for atopic dermatitis prevention |
| title_sort | systemic inflammatory proteins in offspring following maternal probiotic supplementation for atopic dermatitis prevention |
| topic | Atopic dermatitis Biomarkers Children Plasma Probiotics |
| url | https://doi.org/10.1186/s12948-023-00186-3 |
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