The Endopolygalacturonase 1 from Botrytis cinerea Activates Grapevine Defense Reactions Unrelated to Its Enzymatic Activity
A purified glycoprotein from Botrytis cinerea(strain T4), identified as endopolygalacturonase 1 (T4BcPG1) by mass spectrometry analysis, has been shown to activate defense reactions in grapevine (Vitis vinifera cv. Gamay). These reactions include calcium influx, production of active oxygen species,...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
The American Phytopathological Society
2003-06-01
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| Series: | Molecular Plant-Microbe Interactions |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI.2003.16.6.553 |
| _version_ | 1811264535363846144 |
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| author | Benoît Poinssot Elodie Vandelle Marc Bentéjac Marielle Adrian Caroline Levis Yves Brygoo Jérome Garin Francesca Sicilia Pierre Coutos-Thévenot Alain Pugin |
| author_facet | Benoît Poinssot Elodie Vandelle Marc Bentéjac Marielle Adrian Caroline Levis Yves Brygoo Jérome Garin Francesca Sicilia Pierre Coutos-Thévenot Alain Pugin |
| author_sort | Benoît Poinssot |
| collection | DOAJ |
| description | A purified glycoprotein from Botrytis cinerea(strain T4), identified as endopolygalacturonase 1 (T4BcPG1) by mass spectrometry analysis, has been shown to activate defense reactions in grapevine (Vitis vinifera cv. Gamay). These reactions include calcium influx, production of active oxygen species, activation of two mitogen-activated protein kinases, defense gene transcript accumulation, and phytoalexin production. Most of these defense reactions were also activated in grapevine in response to purified oligogalacturonides (OGA) with a degree of polymerization of 9 to 20. In vivo, these active OGA might be a part of the released products resulting from endopolygalacturonase activity on plant cell walls. Nevertheless, the intensity and kinetics of events triggered by OGA were very different when compared with T4BcPG1 effects. Moreover, chemical treatments of T4BcPG1 and desensitization assays have allowed us to discriminate enzymatic and elicitor activities, indicating that elicitor activity was not due to released oligogalacturonides. Thus, BcPG1 should be considered as both an avirulence and a virulence factor. The role of the secreted BcPG1 in the pathogenicity of Botrytis cinerea is discussed. |
| first_indexed | 2024-04-12T20:06:05Z |
| format | Article |
| id | doaj.art-bc42f206483f4560946ea9295fb6787d |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-12T20:06:05Z |
| publishDate | 2003-06-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-bc42f206483f4560946ea9295fb6787d2022-12-22T03:18:24ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062003-06-0116655356410.1094/MPMI.2003.16.6.553The Endopolygalacturonase 1 from Botrytis cinerea Activates Grapevine Defense Reactions Unrelated to Its Enzymatic ActivityBenoît PoinssotElodie VandelleMarc BentéjacMarielle AdrianCaroline LevisYves BrygooJérome GarinFrancesca SiciliaPierre Coutos-ThévenotAlain PuginA purified glycoprotein from Botrytis cinerea(strain T4), identified as endopolygalacturonase 1 (T4BcPG1) by mass spectrometry analysis, has been shown to activate defense reactions in grapevine (Vitis vinifera cv. Gamay). These reactions include calcium influx, production of active oxygen species, activation of two mitogen-activated protein kinases, defense gene transcript accumulation, and phytoalexin production. Most of these defense reactions were also activated in grapevine in response to purified oligogalacturonides (OGA) with a degree of polymerization of 9 to 20. In vivo, these active OGA might be a part of the released products resulting from endopolygalacturonase activity on plant cell walls. Nevertheless, the intensity and kinetics of events triggered by OGA were very different when compared with T4BcPG1 effects. Moreover, chemical treatments of T4BcPG1 and desensitization assays have allowed us to discriminate enzymatic and elicitor activities, indicating that elicitor activity was not due to released oligogalacturonides. Thus, BcPG1 should be considered as both an avirulence and a virulence factor. The role of the secreted BcPG1 in the pathogenicity of Botrytis cinerea is discussed.https://apsjournals.apsnet.org/doi/10.1094/MPMI.2003.16.6.553 |
| spellingShingle | Benoît Poinssot Elodie Vandelle Marc Bentéjac Marielle Adrian Caroline Levis Yves Brygoo Jérome Garin Francesca Sicilia Pierre Coutos-Thévenot Alain Pugin The Endopolygalacturonase 1 from Botrytis cinerea Activates Grapevine Defense Reactions Unrelated to Its Enzymatic Activity Molecular Plant-Microbe Interactions |
| title | The Endopolygalacturonase 1 from Botrytis cinerea Activates Grapevine Defense Reactions Unrelated to Its Enzymatic Activity |
| title_full | The Endopolygalacturonase 1 from Botrytis cinerea Activates Grapevine Defense Reactions Unrelated to Its Enzymatic Activity |
| title_fullStr | The Endopolygalacturonase 1 from Botrytis cinerea Activates Grapevine Defense Reactions Unrelated to Its Enzymatic Activity |
| title_full_unstemmed | The Endopolygalacturonase 1 from Botrytis cinerea Activates Grapevine Defense Reactions Unrelated to Its Enzymatic Activity |
| title_short | The Endopolygalacturonase 1 from Botrytis cinerea Activates Grapevine Defense Reactions Unrelated to Its Enzymatic Activity |
| title_sort | endopolygalacturonase 1 from botrytis cinerea activates grapevine defense reactions unrelated to its enzymatic activity |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI.2003.16.6.553 |
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