In Vitro Antifungal Activity of <i>Peltophorum dubium</i> (Spreng.) Taub. extracts against <i>Aspergillus flavus</i>
<i>Aspergillus flavus</i> is a filamentous, saprophytic fungus, whose colonization occurs mainly in cereal grains and oilseeds once harvested. Under certain conditions, it could produce mycotoxins called aflatoxins, known as powerful human liver carcinogens. The aim of the present study...
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MDPI AG
2020-04-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/9/4/438 |
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| author | Lucía S. Di Ciaccio Alejandra V. Catalano Paula G. López Dante Rojas Diego Cristos Renée H. Fortunato Adriana E. Salvat |
| author_facet | Lucía S. Di Ciaccio Alejandra V. Catalano Paula G. López Dante Rojas Diego Cristos Renée H. Fortunato Adriana E. Salvat |
| author_sort | Lucía S. Di Ciaccio |
| collection | DOAJ |
| description | <i>Aspergillus flavus</i> is a filamentous, saprophytic fungus, whose colonization occurs mainly in cereal grains and oilseeds once harvested. Under certain conditions, it could produce mycotoxins called aflatoxins, known as powerful human liver carcinogens. The aim of the present study was to describe the antifungal activity of extracts of <i>Peltophorum dubium</i>, a species from northern Argentina (Oriental Chaco), against <i>A. flavus</i>. The antifungal activities of different collection sites are reported. The extracts exhibited a minimum inhibitory concentration of 125 µg/mL, and the differences between the treatments and the inoculum control were 11 mm of <i>P. dubium A</i> and 10 mm of <i>P. dubium F</i> in colony growth. Moreover, hyphae treated with the extracts stained blue with Evans blue showed alterations in the membrane and/or cell wall, allowing the dye income. Bio-guided fractionation, High Performance Liquid Chromatography diode array ultraviolet/visible (HPLC UV/VIS DAD), and Ultra-High Performance Liquid Chromatography Electrospray Ionization Mass Spectrometry (UPLC ESI-MS) analyses were conducted to characterize the extracts and their active fractions. The HPLC UV/VIS DAD analysis allowed the determination of the presence of flavonoids (flavonols and flavones), coumarins, terpenes, and steroids. UPLC ESI/MS analysis of active fractions revealed the presence of Kaempferol, Apigenin, Naringenin, Chrysin and Daidzein. |
| first_indexed | 2024-03-10T20:44:13Z |
| format | Article |
| id | doaj.art-bc4394059cc94f049bdc314b97f56dcc |
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| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T20:44:13Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-bc4394059cc94f049bdc314b97f56dcc2023-11-19T20:27:20ZengMDPI AGPlants2223-77472020-04-019443810.3390/plants9040438In Vitro Antifungal Activity of <i>Peltophorum dubium</i> (Spreng.) Taub. extracts against <i>Aspergillus flavus</i>Lucía S. Di Ciaccio0Alejandra V. Catalano1Paula G. López2Dante Rojas3Diego Cristos4Renée H. Fortunato5Adriana E. Salvat6Instituto de Patobiología Veterinaria, (IPvet), CICVyA, Instituto Nacional de Tecnología Agropecuaria, Hurlingham 1686, Prov. de Buenos Aires, ArgentinaConsejo Nacional de Investigaciones Científicas y Técnicas—CONICET, Ciudad Autónoma de Buenos Aires 1425, ArgentinaConsejo Nacional de Investigaciones Científicas y Técnicas—CONICET, Ciudad Autónoma de Buenos Aires 1425, ArgentinaInstituto de Tecnología de Alimentos, Instituto Nacional de Tecnología Agropecuaria, Hurlingham 1686, Prov. de Buenos Aires, ArgentinaInstituto de Tecnología de Alimentos, Instituto Nacional de Tecnología Agropecuaria, Hurlingham 1686, Prov. de Buenos Aires, ArgentinaConsejo Nacional de Investigaciones Científicas y Técnicas—CONICET, Ciudad Autónoma de Buenos Aires 1425, ArgentinaInstituto de Patobiología Veterinaria, (IPvet), CICVyA, Instituto Nacional de Tecnología Agropecuaria, Hurlingham 1686, Prov. de Buenos Aires, Argentina<i>Aspergillus flavus</i> is a filamentous, saprophytic fungus, whose colonization occurs mainly in cereal grains and oilseeds once harvested. Under certain conditions, it could produce mycotoxins called aflatoxins, known as powerful human liver carcinogens. The aim of the present study was to describe the antifungal activity of extracts of <i>Peltophorum dubium</i>, a species from northern Argentina (Oriental Chaco), against <i>A. flavus</i>. The antifungal activities of different collection sites are reported. The extracts exhibited a minimum inhibitory concentration of 125 µg/mL, and the differences between the treatments and the inoculum control were 11 mm of <i>P. dubium A</i> and 10 mm of <i>P. dubium F</i> in colony growth. Moreover, hyphae treated with the extracts stained blue with Evans blue showed alterations in the membrane and/or cell wall, allowing the dye income. Bio-guided fractionation, High Performance Liquid Chromatography diode array ultraviolet/visible (HPLC UV/VIS DAD), and Ultra-High Performance Liquid Chromatography Electrospray Ionization Mass Spectrometry (UPLC ESI-MS) analyses were conducted to characterize the extracts and their active fractions. The HPLC UV/VIS DAD analysis allowed the determination of the presence of flavonoids (flavonols and flavones), coumarins, terpenes, and steroids. UPLC ESI/MS analysis of active fractions revealed the presence of Kaempferol, Apigenin, Naringenin, Chrysin and Daidzein.https://www.mdpi.com/2223-7747/9/4/438<i>Aspergillus flavus</i>flavonoidsplant bioactivityevans blueactive extract characterization |
| spellingShingle | Lucía S. Di Ciaccio Alejandra V. Catalano Paula G. López Dante Rojas Diego Cristos Renée H. Fortunato Adriana E. Salvat In Vitro Antifungal Activity of <i>Peltophorum dubium</i> (Spreng.) Taub. extracts against <i>Aspergillus flavus</i> Plants <i>Aspergillus flavus</i> flavonoids plant bioactivity evans blue active extract characterization |
| title | In Vitro Antifungal Activity of <i>Peltophorum dubium</i> (Spreng.) Taub. extracts against <i>Aspergillus flavus</i> |
| title_full | In Vitro Antifungal Activity of <i>Peltophorum dubium</i> (Spreng.) Taub. extracts against <i>Aspergillus flavus</i> |
| title_fullStr | In Vitro Antifungal Activity of <i>Peltophorum dubium</i> (Spreng.) Taub. extracts against <i>Aspergillus flavus</i> |
| title_full_unstemmed | In Vitro Antifungal Activity of <i>Peltophorum dubium</i> (Spreng.) Taub. extracts against <i>Aspergillus flavus</i> |
| title_short | In Vitro Antifungal Activity of <i>Peltophorum dubium</i> (Spreng.) Taub. extracts against <i>Aspergillus flavus</i> |
| title_sort | in vitro antifungal activity of i peltophorum dubium i spreng taub extracts against i aspergillus flavus i |
| topic | <i>Aspergillus flavus</i> flavonoids plant bioactivity evans blue active extract characterization |
| url | https://www.mdpi.com/2223-7747/9/4/438 |
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