Differential expression of novel potential regulators in hematopoietic stem cells.
The hematopoietic system is an invaluable model both for understanding basic developmental biology and for developing clinically relevant cell therapies. Using highly purified cells and rigorous microarray analysis we have compared the expression pattern of three of the most primitive hematopoietic...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2005-09-01
|
| Series: | PLoS Genetics |
| Online Access: | http://europepmc.org/articles/PMC1200425?pdf=render |
| _version_ | 1818565372441264128 |
|---|---|
| author | E Camilla Forsberg Susan S Prohaska Sol Katzman Garrett C Heffner Josh M Stuart Irving L Weissman |
| author_facet | E Camilla Forsberg Susan S Prohaska Sol Katzman Garrett C Heffner Josh M Stuart Irving L Weissman |
| author_sort | E Camilla Forsberg |
| collection | DOAJ |
| description | The hematopoietic system is an invaluable model both for understanding basic developmental biology and for developing clinically relevant cell therapies. Using highly purified cells and rigorous microarray analysis we have compared the expression pattern of three of the most primitive hematopoietic subpopulations in adult mouse bone marrow: long-term hematopoietic stem cells (HSC), short-term HSC, and multipotent progenitors. All three populations are capable of differentiating into a spectrum of mature blood cells, but differ in their self-renewal and proliferative capacity. We identified numerous novel potential regulators of HSC self-renewal and proliferation that were differentially expressed between these closely related cell populations. Many of the differentially expressed transcripts fit into pathways and protein complexes not previously identified in HSC, providing evidence for new HSC regulatory units. Extending these observations to the protein level, we demonstrate expression of several of the corresponding proteins, which provide novel surface markers for HSC. We discuss the implications of our findings for HSC biology. In particular, our data suggest that cell-cell and cell-matrix interactions are major regulators of long-term HSC, and that HSC themselves play important roles in regulating their immediate microenvironment. |
| first_indexed | 2024-12-14T01:40:23Z |
| format | Article |
| id | doaj.art-bc4ad70ba49341c7af1f1034ca399145 |
| institution | Directory Open Access Journal |
| issn | 1553-7390 1553-7404 |
| language | English |
| last_indexed | 2024-12-14T01:40:23Z |
| publishDate | 2005-09-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj.art-bc4ad70ba49341c7af1f1034ca3991452022-12-21T23:21:46ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042005-09-0113e2810.1371/journal.pgen.0010028Differential expression of novel potential regulators in hematopoietic stem cells.E Camilla ForsbergSusan S ProhaskaSol KatzmanGarrett C HeffnerJosh M StuartIrving L WeissmanThe hematopoietic system is an invaluable model both for understanding basic developmental biology and for developing clinically relevant cell therapies. Using highly purified cells and rigorous microarray analysis we have compared the expression pattern of three of the most primitive hematopoietic subpopulations in adult mouse bone marrow: long-term hematopoietic stem cells (HSC), short-term HSC, and multipotent progenitors. All three populations are capable of differentiating into a spectrum of mature blood cells, but differ in their self-renewal and proliferative capacity. We identified numerous novel potential regulators of HSC self-renewal and proliferation that were differentially expressed between these closely related cell populations. Many of the differentially expressed transcripts fit into pathways and protein complexes not previously identified in HSC, providing evidence for new HSC regulatory units. Extending these observations to the protein level, we demonstrate expression of several of the corresponding proteins, which provide novel surface markers for HSC. We discuss the implications of our findings for HSC biology. In particular, our data suggest that cell-cell and cell-matrix interactions are major regulators of long-term HSC, and that HSC themselves play important roles in regulating their immediate microenvironment.http://europepmc.org/articles/PMC1200425?pdf=render |
| spellingShingle | E Camilla Forsberg Susan S Prohaska Sol Katzman Garrett C Heffner Josh M Stuart Irving L Weissman Differential expression of novel potential regulators in hematopoietic stem cells. PLoS Genetics |
| title | Differential expression of novel potential regulators in hematopoietic stem cells. |
| title_full | Differential expression of novel potential regulators in hematopoietic stem cells. |
| title_fullStr | Differential expression of novel potential regulators in hematopoietic stem cells. |
| title_full_unstemmed | Differential expression of novel potential regulators in hematopoietic stem cells. |
| title_short | Differential expression of novel potential regulators in hematopoietic stem cells. |
| title_sort | differential expression of novel potential regulators in hematopoietic stem cells |
| url | http://europepmc.org/articles/PMC1200425?pdf=render |
| work_keys_str_mv | AT ecamillaforsberg differentialexpressionofnovelpotentialregulatorsinhematopoieticstemcells AT susansprohaska differentialexpressionofnovelpotentialregulatorsinhematopoieticstemcells AT solkatzman differentialexpressionofnovelpotentialregulatorsinhematopoieticstemcells AT garrettcheffner differentialexpressionofnovelpotentialregulatorsinhematopoieticstemcells AT joshmstuart differentialexpressionofnovelpotentialregulatorsinhematopoieticstemcells AT irvinglweissman differentialexpressionofnovelpotentialregulatorsinhematopoieticstemcells |