Passaging Primary Human Bronchial Epithelia Reduces CFTR-Mediated Fluid Transport and Alters mRNA Expression

Passaging Primary Human Bronchial Epithelia Reduces CFTR-Mediated Fluid Transport and Alters mRNA Expression

Primary human bronchial epithelial cultures (HBECs) are used to study airway physiology, disease, and drug development. HBECs often replicate human airway physiology/pathophysiology. Indeed, in the search for cystic fibrosis (CF) transmembrane conductance regulator (CFTR) therapies, HBECs were seen...

Volledige beschrijving

Bibliografische gegevens
Hoofdauteurs:	Tongde Wu, Joe A. Wrennall, Hong Dang, Deborah L. Baines, Robert Tarran
Formaat:	Artikel
Taal:	English
Gepubliceerd in:	MDPI AG 2023-03-01
Reeks:	Cells
Onderwerpen:	airway surface liquid tight junction membrane transport Ussing chamber water permeability
Online toegang:	https://www.mdpi.com/2073-4409/12/7/997

Gelijkaardige items

Acute Effects of Butyrate on Induced Hyperpermeability and Tight Junction Protein Expression in Human Colonic Tissues
door: Mathias W. Tabat, et al.
Gepubliceerd in: (2020-05-01)

Dysregulation of Intestinal Physiology by Aflatoxicosis in the Gilthead Seabream (Sparus aurata)
door: Andre Barany, et al.
Gepubliceerd in: (2021-12-01)

Transport and Barrier Functions in Rainbow Trout Trunk Skin Are Regulated by Environmental Salinity
door: D. Doyle, et al.
Gepubliceerd in: (2022-05-01)

Dose and time dependence of functional impairments in rat jejunum following ionizing radiation exposure
door: Alexandra A. Livanova, et al.
Gepubliceerd in: (2021-08-01)

Permeability of the Retina and RPE-Choroid-Sclera to Three Ophthalmic Drugs and the Associated Factors
door: Hyeong Min Kim, et al.
Gepubliceerd in: (2021-05-01)

Simple graphical approach to investigate differences in transepithelial paracellular leak pathway permeability
door: Ashley Monaco, et al.
Gepubliceerd in: (2022-03-01)

Taste Receptor Activation in Tracheal Brush Cells by Denatonium Modulates ENaC Channels via Ca<sup>2+</sup>, cAMP and ACh
door: Monika I. Hollenhorst, et al.
Gepubliceerd in: (2022-08-01)

Intestinal Membrane Function in Inflammatory Bowel Disease
door: Daisuke Nakai, et al.
Gepubliceerd in: (2023-12-01)

Changes in Intestinal Permeability Ex Vivo and Immune Cell Activation by Three Commonly Used Emulsifiers
door: Elin Oscarsson, et al.
Gepubliceerd in: (2020-12-01)

Absorption and metabolism of volatile fatty acids by rumen and omasum Absorção e metabolismo de ácidos graxos voláteis pelo rúmen e omaso
door: João Luiz Pratti Daniel, et al.
Gepubliceerd in: (2012-02-01)

Ussing's 'Little Chamber': 60 Years+ old and Counting
door: Kirk L Hamilton
Gepubliceerd in: (2011-03-01)

The effects of amiloride and age on oxygen consumption coupled to electrogenic sodium transport in the human sigmoid colon
door: Graciela E Carra, et al.
Gepubliceerd in: (2015-01-01)

Alteration in the mRNA expression of genes associated with gastrointestinal permeability and ileal TNF-α secretion due to the exposure of silver nanoparticles in Sprague–Dawley rats
door: Sarah E. Orr, et al.
Gepubliceerd in: (2019-05-01)

Electrogenic transport of amino acids and glucose differs between jejunum and ileum of female and castrated male finishing pigs
door: Marco Tretola, et al.
Gepubliceerd in: (2024-12-01)

Design of using chamber system based on electrical impedance spectroscopy (EIS) to measure epithelial tissue
door: Paulo C. Calvo, et al.
Gepubliceerd in: (2020-08-01)

Preventive effects of royal jelly against anaphylactic response in a murine model of cow’s milk allergy
door: Malika Guendouz, et al.
Gepubliceerd in: (2017-01-01)

Porcine Colostrum Protects the IPEC-J2 Cells and Piglet Colon Epithelium against <i>Clostridioides</i> (syn. <i>Clostridium</i>) <i>difficile</i> Toxin-Induced Effects
door: Łukasz Grześkowiak, et al.
Gepubliceerd in: (2020-01-01)

Using Porcine Jejunum Ex Vivo to Study Absorption and Biotransformation of Natural Products in Plant Extracts: <i>Pueraria lobata</i> as a Case Study
door: Joëlle Houriet, et al.
Gepubliceerd in: (2021-08-01)

An Antioxidant Supplement Function Exploration: Rescue of Intestinal Structure Injury by Mannan Oligosaccharides after <i>Aeromonas hydrophila</i> Infection in Grass Carp (<i>Ctenopharyngodon idella</i>)
door: Zhi-Yuan Lu, et al.
Gepubliceerd in: (2022-04-01)

The panda-derived Lactiplantibacillus plantarum BSG201683 improves LPS-induced intestinal inflammation and epithelial barrier disruption in vitro
door: Yi Zhou, et al.
Gepubliceerd in: (2023-09-01)

Effect of nano- and micro-polystyrene particles on small intestinal epithelial functions and enteric neuronal activity in vitro
door: Kristin Elfers, et al.
Gepubliceerd in: (2025-02-01)

Two mechanisms of membrane digestion: enzymatic-transport ensemble exists
door: S. T. Metelsky, et al.
Gepubliceerd in: (2010-11-01)

L-Tryptophan Differentially Regulated Glucose and Amino Acid Transporters in the Small Intestine of Rat Challenged with Lipopolysaccharide
door: Bin Wang, et al.
Gepubliceerd in: (2022-11-01)

Does Deoxynivalenol Affect Amoxicillin and Doxycycline Absorption in the Gastrointestinal Tract? Ex Vivo Study on Swine Jejunum Mucosa Explants
door: Marta Mendel, et al.
Gepubliceerd in: (2022-10-01)

Physiological bioactivity of a postbiotic consisting of heat-treated lactobacilli on mouse small intestine
door: Friederike Uhlig, et al.
Gepubliceerd in: (2023-09-01)

A review on the evolution of methods for intestinal in vitro organ culture and its application in veterinary science
door: Barbara Ribeiro de Souza Cortez, et al.
Gepubliceerd in: (2023-02-01)

Cryptosporidium parvum competes with the intestinal epithelial cells for glucose and impairs systemic glucose supply in neonatal calves
door: Franziska Dengler, et al.
Gepubliceerd in: (2023-05-01)

The effect of Campylobacter jejuni and Campylobacter coli colonization on the gut morphology, functional integrity, and microbiota composition of female turkeys
door: Janina Rzeznitzeck, et al.
Gepubliceerd in: (2022-08-01)

Effect of sucralfate on gastric permeability in an ex vivo model of stress‐related mucosal disease in dogs
door: Tracy L. Hill, et al.
Gepubliceerd in: (2018-03-01)

Impact of <i>Campylobacter</i> spp. on the Integrity of the Porcine Gut
door: Alexandra Rath, et al.
Gepubliceerd in: (2021-09-01)

Drug Transport across Porcine Intestine Using an Ussing Chamber System: Regional Differences and the Effect of P-Glycoprotein and CYP3A4 Activity on Drug Absorption
door: Yvonne E. Arnold, et al.
Gepubliceerd in: (2019-03-01)

Regulation of Epithelial and Endothelial Barriers by Molecular Chaperones
door: Susana Lechuga, et al.
Gepubliceerd in: (2024-02-01)

Ex vivo and in vitro poultry intestinal models to evaluate antimycotoxins additives
door: Vinicius Duarte, et al.
Gepubliceerd in: (2021-11-01)

Experimental evidence that polystyrene nanoplastics cross the intestinal barrier of European seabass
door: M. Vagner, et al.
Gepubliceerd in: (2022-08-01)

Interactions Between Emodin and Efflux Transporters on Rat Enterocyte by a Validated Ussing Chamber Technique
door: Juan Huang, et al.
Gepubliceerd in: (2018-06-01)

Osteopontin (OPN)/Secreted Phosphoprotein 1 (SPP1) Binds Integrins to Activate Transport of Ions Across the Porcine Placenta
door: Guoyao Wu, et al.
Gepubliceerd in: (2022-04-01)

Assessment of Distinct Electrophysiological Parameters in Rectal Biopsies for the Choice of the Best Diagnosis/Prognosis Biomarkers for Cystic Fibrosis
door: Iris A. L. Silva, et al.
Gepubliceerd in: (2020-12-01)

Reconstitution of functional tight junctions with individual claudin subtypes in epithelial cells
door: Mikio Furuse, et al.
Gepubliceerd in: (2022-12-01)

Immunosuppressant MPA Modulates Tight Junction through Epigenetic Activation of MLCK/MLC-2 Pathway via p38MAPK
door: Niamat Khan, et al.
Gepubliceerd in: (2015-12-01)

Homocysteine Aggravates Intestinal Epithelial Barrier Dysfunction in Rats with Experimental Uremia
door: Shanshan Liang, et al.
Gepubliceerd in: (2018-10-01)