Effects of platelet‐rich plasma on the memory impairment, apoptosis, and hippocampal synaptic plasticity in a rat model of hepatic encephalopathy
Abstract Objectives In the present study, we aimed to determine whether intraperitoneal injection of platelet‐rich plasma (PRP) could have a neuroprotective effect on learning, memory, and synaptic plasticity impairment as well as hippocampal apoptosis in rats with hepatic encephalopathy induced by...
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | Brain and Behavior |
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| Online Access: | https://doi.org/10.1002/brb3.2447 |
| _version_ | 1819320069008654336 |
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| author | Mahnaz Bayat Azadeh Khalili Gholamreza Bayat Somayeh Akbari Amirhossein Yousefi Nejad Afshin Borhani Haghighi Masoud Haghani |
| author_facet | Mahnaz Bayat Azadeh Khalili Gholamreza Bayat Somayeh Akbari Amirhossein Yousefi Nejad Afshin Borhani Haghighi Masoud Haghani |
| author_sort | Mahnaz Bayat |
| collection | DOAJ |
| description | Abstract Objectives In the present study, we aimed to determine whether intraperitoneal injection of platelet‐rich plasma (PRP) could have a neuroprotective effect on learning, memory, and synaptic plasticity impairment as well as hippocampal apoptosis in rats with hepatic encephalopathy induced by bile duct ligated (BDL). Methods The rats were divided into four groups: the control, sham, BDL+ V (vehicle), and BDL+ PRP. The BDL rats were treated with PRP immediately after the surgery, and the injection was done every 3 days for 30 days. The passive avoidance and Morris water maze tests were used for the evaluation of learning and memory. The long‐term potentiation (LTP), basal‐synaptic transmission, and paired‐pulse ratio, as an index for measurement of neurotransmitter release probability, were evaluated by field‐potential recording. After taking a blood sample for assessment of the liver enzymes, the animals were sacrificed and their hippocampus was removed for evaluation of cleaved caspase‐3 by Western blot. Results Serological assessment of the liver function showed that BDL severely impaired the liver function. Also, PRP treatment could partially improve the liver dysfunction along with recovery in fear memory and spatial learning memory performance, LTP, basal‐synaptic transmission, and neurotransmitter release probability. PRP‐treated rats also showed a significant reduction in neuronal apoptosis in the CA1 area. Conclusions The results of this study suggest that PRP improves cognitive performance and synaptic plasticity in BDL rats via direct neuroprotective property and/or indirectly by improvement of hepatic dysfunction. |
| first_indexed | 2024-12-24T11:13:43Z |
| format | Article |
| id | doaj.art-bc4f1c4f43ef47bf92672c919ccb9b90 |
| institution | Directory Open Access Journal |
| issn | 2162-3279 |
| language | English |
| last_indexed | 2024-12-24T11:13:43Z |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Brain and Behavior |
| spelling | doaj.art-bc4f1c4f43ef47bf92672c919ccb9b902022-12-21T16:58:27ZengWileyBrain and Behavior2162-32792022-01-01121n/an/a10.1002/brb3.2447Effects of platelet‐rich plasma on the memory impairment, apoptosis, and hippocampal synaptic plasticity in a rat model of hepatic encephalopathyMahnaz Bayat0Azadeh Khalili1Gholamreza Bayat2Somayeh Akbari3Amirhossein Yousefi Nejad4Afshin Borhani Haghighi5Masoud Haghani6Clinical Neurology Research Centre Shiraz University of Medical Sciences Shiraz IranDepartment of Physiology‐Pharmacology‐Medical Physic School of Medicine Alborz University of Medical Sciences Karaj IranDepartment of Physiology‐Pharmacology‐Medical Physic School of Medicine Alborz University of Medical Sciences Karaj IranDepartment of Physiology The Medical School Shiraz University of Medical Sciences Shiraz IranFaculty of Veterinary Medicine, Department of Veterinary Medicine Islamic Azad University of Kazeroon Shiraz IranClinical Neurology Research Centre Shiraz University of Medical Sciences Shiraz IranDepartment of Physiology The Medical School Shiraz University of Medical Sciences Shiraz IranAbstract Objectives In the present study, we aimed to determine whether intraperitoneal injection of platelet‐rich plasma (PRP) could have a neuroprotective effect on learning, memory, and synaptic plasticity impairment as well as hippocampal apoptosis in rats with hepatic encephalopathy induced by bile duct ligated (BDL). Methods The rats were divided into four groups: the control, sham, BDL+ V (vehicle), and BDL+ PRP. The BDL rats were treated with PRP immediately after the surgery, and the injection was done every 3 days for 30 days. The passive avoidance and Morris water maze tests were used for the evaluation of learning and memory. The long‐term potentiation (LTP), basal‐synaptic transmission, and paired‐pulse ratio, as an index for measurement of neurotransmitter release probability, were evaluated by field‐potential recording. After taking a blood sample for assessment of the liver enzymes, the animals were sacrificed and their hippocampus was removed for evaluation of cleaved caspase‐3 by Western blot. Results Serological assessment of the liver function showed that BDL severely impaired the liver function. Also, PRP treatment could partially improve the liver dysfunction along with recovery in fear memory and spatial learning memory performance, LTP, basal‐synaptic transmission, and neurotransmitter release probability. PRP‐treated rats also showed a significant reduction in neuronal apoptosis in the CA1 area. Conclusions The results of this study suggest that PRP improves cognitive performance and synaptic plasticity in BDL rats via direct neuroprotective property and/or indirectly by improvement of hepatic dysfunction.https://doi.org/10.1002/brb3.2447field potential recordinghepatic encephalopathyLTPplatelet‐rich plasma |
| spellingShingle | Mahnaz Bayat Azadeh Khalili Gholamreza Bayat Somayeh Akbari Amirhossein Yousefi Nejad Afshin Borhani Haghighi Masoud Haghani Effects of platelet‐rich plasma on the memory impairment, apoptosis, and hippocampal synaptic plasticity in a rat model of hepatic encephalopathy Brain and Behavior field potential recording hepatic encephalopathy LTP platelet‐rich plasma |
| title | Effects of platelet‐rich plasma on the memory impairment, apoptosis, and hippocampal synaptic plasticity in a rat model of hepatic encephalopathy |
| title_full | Effects of platelet‐rich plasma on the memory impairment, apoptosis, and hippocampal synaptic plasticity in a rat model of hepatic encephalopathy |
| title_fullStr | Effects of platelet‐rich plasma on the memory impairment, apoptosis, and hippocampal synaptic plasticity in a rat model of hepatic encephalopathy |
| title_full_unstemmed | Effects of platelet‐rich plasma on the memory impairment, apoptosis, and hippocampal synaptic plasticity in a rat model of hepatic encephalopathy |
| title_short | Effects of platelet‐rich plasma on the memory impairment, apoptosis, and hippocampal synaptic plasticity in a rat model of hepatic encephalopathy |
| title_sort | effects of platelet rich plasma on the memory impairment apoptosis and hippocampal synaptic plasticity in a rat model of hepatic encephalopathy |
| topic | field potential recording hepatic encephalopathy LTP platelet‐rich plasma |
| url | https://doi.org/10.1002/brb3.2447 |
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