Advances in <i>Azorella glabra</i> Wedd. Extract Research: In Vitro Antioxidant Activity, Antiproliferative Effects on Acute Myeloid Leukemia Cells and Bioactive Compound Characterization
<i>Azorella glabra</i> Wedd. (AG) is traditionally used to treat gonorrhea or kidney’s problems. The antioxidant, antidiabetic, anticholinesterase and in vitro antitumor activities of AG extracts were recently reported. The aim of this work was to investigate anti-leukemic properties of...
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MDPI AG
2020-10-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/21/4890 |
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| author | Daniela Lamorte Immacolata Faraone Ilaria Laurenzana Stefania Trino Daniela Russo Dilip K. Rai Maria Francesca Armentano Pellegrino Musto Alessandro Sgambato Luciana De Luca Luigi Milella Antonella Caivano |
| author_facet | Daniela Lamorte Immacolata Faraone Ilaria Laurenzana Stefania Trino Daniela Russo Dilip K. Rai Maria Francesca Armentano Pellegrino Musto Alessandro Sgambato Luciana De Luca Luigi Milella Antonella Caivano |
| author_sort | Daniela Lamorte |
| collection | DOAJ |
| description | <i>Azorella glabra</i> Wedd. (AG) is traditionally used to treat gonorrhea or kidney’s problems. The antioxidant, antidiabetic, anticholinesterase and in vitro antitumor activities of AG extracts were recently reported. The aim of this work was to investigate anti-leukemic properties of AG chloroform fraction (AG CHCl<sub>3</sub>) and of its ten sub-fractions (I-X) and to identify their possible bioactive compounds. We determined their in vitro antioxidant activity using 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), nitric oxide (NO) and superoxide anion (SO) assays, and their phytochemical profile by spectrophotometric and LC-MS/MS techniques. I-X action on two acute myeloid leukemia (AML) cell lines viability, apoptosis and cell cycle were evaluated by MTS, western blotting and cytofluorimetric assays. Different polyphenol, flavonoid and terpenoid amount, and antioxidant activity were found among all samples. Most of I-X induced a dose/time dependent reduction of cell viability higher than parent extract. IV and VI sub-fractions showed highest cytotoxic activity and, of note, a negligible reduction of healthy cell viability. They activated intrinsic apoptotic pathway, induced a G<sub>0</sub>/G<sub>1</sub> block in leukemic cells and, interestingly, led to apoptosis in patient AML cells. These activities could be due to mulinic acid or azorellane terpenoids and their derivatives, tentatively identified in both IV and VI. In conclusion, our data suggest AG plant as a source of potential anti-AML agents. |
| first_indexed | 2024-03-10T15:24:55Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T15:24:55Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-bc4fce272e444d40895d54828289b19c2023-11-20T18:10:11ZengMDPI AGMolecules1420-30492020-10-012521489010.3390/molecules25214890Advances in <i>Azorella glabra</i> Wedd. Extract Research: In Vitro Antioxidant Activity, Antiproliferative Effects on Acute Myeloid Leukemia Cells and Bioactive Compound CharacterizationDaniela Lamorte0Immacolata Faraone1Ilaria Laurenzana2Stefania Trino3Daniela Russo4Dilip K. Rai5Maria Francesca Armentano6Pellegrino Musto7Alessandro Sgambato8Luciana De Luca9Luigi Milella10Antonella Caivano11Laboratory of Preclinical and Translational Research, Centro di Riferimento Oncologico della Basilicata (IRCCS CROB), 85028 Rionero in Vulture, Potenza, ItalyDepartment of Science, University of Basilicata, V.le dell’Ateneo Lucano 10, 85028 Rionero in Vulture, Potenza, ItalyLaboratory of Preclinical and Translational Research, Centro di Riferimento Oncologico della Basilicata (IRCCS CROB), 85028 Rionero in Vulture, Potenza, ItalyLaboratory of Preclinical and Translational Research, Centro di Riferimento Oncologico della Basilicata (IRCCS CROB), 85028 Rionero in Vulture, Potenza, ItalyDepartment of Science, University of Basilicata, V.le dell’Ateneo Lucano 10, 85028 Rionero in Vulture, Potenza, ItalyDepartment of Food BioSciences, Teagasc Food Research Centre Ashtown, D15KN3K Dublin, IrelandDepartment of Science, University of Basilicata, V.le dell’Ateneo Lucano 10, 85028 Rionero in Vulture, Potenza, ItalyHematology and Stem Cell Transplantation Unit, Centro di Riferimento Oncologico della Basilicata (IRCCS CROB), 85028 Rionero in Vulture, Potenza, ItalyScientific Direction, Centro di Riferimento Oncologico della Basilicata (IRCCS CROB), 85028 Rionero in Vulture, Potenza, ItalyUnit of Clinical Pathology, Centro di Riferimento Oncologico della Basilicata (IRCCS CROB), 85028 Rionero in Vulture, Potenza, ItalyDepartment of Science, University of Basilicata, V.le dell’Ateneo Lucano 10, 85028 Rionero in Vulture, Potenza, ItalyUnit of Clinical Pathology, Centro di Riferimento Oncologico della Basilicata (IRCCS CROB), 85028 Rionero in Vulture, Potenza, Italy<i>Azorella glabra</i> Wedd. (AG) is traditionally used to treat gonorrhea or kidney’s problems. The antioxidant, antidiabetic, anticholinesterase and in vitro antitumor activities of AG extracts were recently reported. The aim of this work was to investigate anti-leukemic properties of AG chloroform fraction (AG CHCl<sub>3</sub>) and of its ten sub-fractions (I-X) and to identify their possible bioactive compounds. We determined their in vitro antioxidant activity using 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), nitric oxide (NO) and superoxide anion (SO) assays, and their phytochemical profile by spectrophotometric and LC-MS/MS techniques. I-X action on two acute myeloid leukemia (AML) cell lines viability, apoptosis and cell cycle were evaluated by MTS, western blotting and cytofluorimetric assays. Different polyphenol, flavonoid and terpenoid amount, and antioxidant activity were found among all samples. Most of I-X induced a dose/time dependent reduction of cell viability higher than parent extract. IV and VI sub-fractions showed highest cytotoxic activity and, of note, a negligible reduction of healthy cell viability. They activated intrinsic apoptotic pathway, induced a G<sub>0</sub>/G<sub>1</sub> block in leukemic cells and, interestingly, led to apoptosis in patient AML cells. These activities could be due to mulinic acid or azorellane terpenoids and their derivatives, tentatively identified in both IV and VI. In conclusion, our data suggest AG plant as a source of potential anti-AML agents.https://www.mdpi.com/1420-3049/25/21/4890<i>Azorella glabra</i> Wedd.<i>Azorella diapensioides</i> A. Gray<i>yareta</i>phytochemicalsterpenoidsantioxidant |
| spellingShingle | Daniela Lamorte Immacolata Faraone Ilaria Laurenzana Stefania Trino Daniela Russo Dilip K. Rai Maria Francesca Armentano Pellegrino Musto Alessandro Sgambato Luciana De Luca Luigi Milella Antonella Caivano Advances in <i>Azorella glabra</i> Wedd. Extract Research: In Vitro Antioxidant Activity, Antiproliferative Effects on Acute Myeloid Leukemia Cells and Bioactive Compound Characterization Molecules <i>Azorella glabra</i> Wedd. <i>Azorella diapensioides</i> A. Gray <i>yareta</i> phytochemicals terpenoids antioxidant |
| title | Advances in <i>Azorella glabra</i> Wedd. Extract Research: In Vitro Antioxidant Activity, Antiproliferative Effects on Acute Myeloid Leukemia Cells and Bioactive Compound Characterization |
| title_full | Advances in <i>Azorella glabra</i> Wedd. Extract Research: In Vitro Antioxidant Activity, Antiproliferative Effects on Acute Myeloid Leukemia Cells and Bioactive Compound Characterization |
| title_fullStr | Advances in <i>Azorella glabra</i> Wedd. Extract Research: In Vitro Antioxidant Activity, Antiproliferative Effects on Acute Myeloid Leukemia Cells and Bioactive Compound Characterization |
| title_full_unstemmed | Advances in <i>Azorella glabra</i> Wedd. Extract Research: In Vitro Antioxidant Activity, Antiproliferative Effects on Acute Myeloid Leukemia Cells and Bioactive Compound Characterization |
| title_short | Advances in <i>Azorella glabra</i> Wedd. Extract Research: In Vitro Antioxidant Activity, Antiproliferative Effects on Acute Myeloid Leukemia Cells and Bioactive Compound Characterization |
| title_sort | advances in i azorella glabra i wedd extract research in vitro antioxidant activity antiproliferative effects on acute myeloid leukemia cells and bioactive compound characterization |
| topic | <i>Azorella glabra</i> Wedd. <i>Azorella diapensioides</i> A. Gray <i>yareta</i> phytochemicals terpenoids antioxidant |
| url | https://www.mdpi.com/1420-3049/25/21/4890 |
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