Evaluation of Anthelmintic Activity of Different Fractions of Azadirachta indica A. Juss Seed Extract
This investigation was aimed at determination of anthelmintic activity of different fractions of Azadirachta indica seed prepared from the crude aqueous methanol extract (CAME). For this purpose, eggs and adult Haemonchus contortus were exposed to aqueous, ethyl acetate, chloroform and petroleum spi...
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| Format: | Article |
| Language: | English |
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University of Agriculture, Faisalabad
2012-10-01
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| Series: | Pakistan Veterinary Journal |
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| Online Access: | http://pvj.com.pk/pdf-files/32_4/579-583.pdf |
| _version_ | 1819298576392519680 |
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| author | Zafar Iqbal*, Wasim Babar, Zia ud Din Sindhu, Rao Zahid Abbas and Muhammad Sohail Sajid |
| author_facet | Zafar Iqbal*, Wasim Babar, Zia ud Din Sindhu, Rao Zahid Abbas and Muhammad Sohail Sajid |
| author_sort | Zafar Iqbal*, Wasim Babar, Zia ud Din Sindhu, Rao Zahid Abbas and Muhammad Sohail Sajid |
| collection | DOAJ |
| description | This investigation was aimed at determination of anthelmintic activity of different fractions of Azadirachta indica seed prepared from the crude aqueous methanol extract (CAME). For this purpose, eggs and adult Haemonchus contortus were exposed to aqueous, ethyl acetate, chloroform and petroleum spirit fractions of A. indica seeds employing standard bioassays. All the fractions of A. indica seeds exhibited dose and/or time dependent ovicidal and wormicidal effects against H. contortus. The best ovicidal activity was demonstrated by the ethyl acetate fraction with LC50=21.32 µg/ml; whereas, it was 6-14 times lower for the other fractions. Likewise, ethyl acetate fraction @ 50 mg ml-1 exhibited the best wormicidal effects by killing 83% adult H. contortus one hour post-exposure. For in vivo anthelmintic activity faecal egg count reduction test was perform on sheep naturally infected with helminthes. Maximum reduction (98.9%) was observed with CAME. Therefore, it is concluded that future studies aimed at identification of active anthelmintic principles of A. indica may be focused on ethyl acetate fraction of the plant. |
| first_indexed | 2024-12-24T05:32:06Z |
| format | Article |
| id | doaj.art-bc51c17268ed421ca2f903fef9275a61 |
| institution | Directory Open Access Journal |
| issn | 0253-8318 |
| language | English |
| last_indexed | 2024-12-24T05:32:06Z |
| publishDate | 2012-10-01 |
| publisher | University of Agriculture, Faisalabad |
| record_format | Article |
| series | Pakistan Veterinary Journal |
| spelling | doaj.art-bc51c17268ed421ca2f903fef9275a612022-12-21T17:13:08ZengUniversity of Agriculture, FaisalabadPakistan Veterinary Journal0253-83182012-10-01324579583Evaluation of Anthelmintic Activity of Different Fractions of Azadirachta indica A. Juss Seed ExtractZafar Iqbal*, Wasim Babar, Zia ud Din Sindhu, Rao Zahid Abbas and Muhammad Sohail SajidThis investigation was aimed at determination of anthelmintic activity of different fractions of Azadirachta indica seed prepared from the crude aqueous methanol extract (CAME). For this purpose, eggs and adult Haemonchus contortus were exposed to aqueous, ethyl acetate, chloroform and petroleum spirit fractions of A. indica seeds employing standard bioassays. All the fractions of A. indica seeds exhibited dose and/or time dependent ovicidal and wormicidal effects against H. contortus. The best ovicidal activity was demonstrated by the ethyl acetate fraction with LC50=21.32 µg/ml; whereas, it was 6-14 times lower for the other fractions. Likewise, ethyl acetate fraction @ 50 mg ml-1 exhibited the best wormicidal effects by killing 83% adult H. contortus one hour post-exposure. For in vivo anthelmintic activity faecal egg count reduction test was perform on sheep naturally infected with helminthes. Maximum reduction (98.9%) was observed with CAME. Therefore, it is concluded that future studies aimed at identification of active anthelmintic principles of A. indica may be focused on ethyl acetate fraction of the plant.http://pvj.com.pk/pdf-files/32_4/579-583.pdfAnthelminticFractionationHaemonchus contortusNeemPlant extract |
| spellingShingle | Zafar Iqbal*, Wasim Babar, Zia ud Din Sindhu, Rao Zahid Abbas and Muhammad Sohail Sajid Evaluation of Anthelmintic Activity of Different Fractions of Azadirachta indica A. Juss Seed Extract Pakistan Veterinary Journal Anthelmintic Fractionation Haemonchus contortus Neem Plant extract |
| title | Evaluation of Anthelmintic Activity of Different Fractions of Azadirachta indica A. Juss Seed Extract |
| title_full | Evaluation of Anthelmintic Activity of Different Fractions of Azadirachta indica A. Juss Seed Extract |
| title_fullStr | Evaluation of Anthelmintic Activity of Different Fractions of Azadirachta indica A. Juss Seed Extract |
| title_full_unstemmed | Evaluation of Anthelmintic Activity of Different Fractions of Azadirachta indica A. Juss Seed Extract |
| title_short | Evaluation of Anthelmintic Activity of Different Fractions of Azadirachta indica A. Juss Seed Extract |
| title_sort | evaluation of anthelmintic activity of different fractions of azadirachta indica a juss seed extract |
| topic | Anthelmintic Fractionation Haemonchus contortus Neem Plant extract |
| url | http://pvj.com.pk/pdf-files/32_4/579-583.pdf |
| work_keys_str_mv | AT zafariqbalwasimbabarziauddinsindhuraozahidabbasandmuhammadsohailsajid evaluationofanthelminticactivityofdifferentfractionsofazadirachtaindicaajussseedextract |