Anti-inflammatory effect of simvastatin by impeding TNF-α and interleukin-1ß pathways: antiangiogenic activity of simvastatin and simvastatin-loaded silver nanoparticles
AbstractPurpose The present study was carried out to evaluate anti-inflammatory and antiangiogenic attributes of simvastatin and its nanofilms containing silver nanoparticles.Methods Silver nanoparticles and simvastatin-loaded nanocomposite (SNSN) films were formulated by using polymeric solution (p...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2022-12-01
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| Series: | Artificial Cells, Nanomedicine, and Biotechnology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/21691401.2022.2098306 |
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| author | Manal Buabeid El-Shaimaa A. Arafa Hafiza Sidra Yaseen Muhammad Ihtisham Umar Ghulam Murtaza |
| author_facet | Manal Buabeid El-Shaimaa A. Arafa Hafiza Sidra Yaseen Muhammad Ihtisham Umar Ghulam Murtaza |
| author_sort | Manal Buabeid |
| collection | DOAJ |
| description | AbstractPurpose The present study was carried out to evaluate anti-inflammatory and antiangiogenic attributes of simvastatin and its nanofilms containing silver nanoparticles.Methods Silver nanoparticles and simvastatin-loaded nanocomposite (SNSN) films were formulated by using polymeric solution (pectin + sericin) through casting solution method. Different in vitro and in vivo anti-inflammatory assays were performed. In addition, chick chorioallantoic membrane assay (CAM) was also employed for angiogenesis activity.Results FTIR spectra of the film depicted the presence of intact simvastatin. Differential scanning calorimetry exhibited no endothermic expression in F9 film thermogram. The simvastatin release from all films exhibited a burst effect. Cotton-pellet induced granuloma model study showed that high dose of simvastatin and indomethacin produced comparable (p < 0.05) anti-inflammatory effect. Noteworthy, RT-PCR showed dose-dependent, anti-oedematous effect of simvastatin through downregulation of serum TNF-α and interleukin-1ß levels. While results of CAM assay exhibited remarkable anti-angiogenic potential of SNSN films showing dissolved blood vessels network macroscopically.Conclusion To reiterate, simvastatin and its SNSN films can add significant contribution to the field of biomedicines due to their promising anti-inflammatory and antiangiogenic properties, however, clinical studies are required to validate their commercial use. |
| first_indexed | 2024-04-11T12:15:16Z |
| format | Article |
| id | doaj.art-bc521c1bd6c342c29a8b92e605b9d091 |
| institution | Directory Open Access Journal |
| issn | 2169-1401 2169-141X |
| language | English |
| last_indexed | 2024-04-11T12:15:16Z |
| publishDate | 2022-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Artificial Cells, Nanomedicine, and Biotechnology |
| spelling | doaj.art-bc521c1bd6c342c29a8b92e605b9d0912022-12-22T04:24:24ZengTaylor & Francis GroupArtificial Cells, Nanomedicine, and Biotechnology2169-14012169-141X2022-12-0150120821710.1080/21691401.2022.2098306Anti-inflammatory effect of simvastatin by impeding TNF-α and interleukin-1ß pathways: antiangiogenic activity of simvastatin and simvastatin-loaded silver nanoparticlesManal Buabeid0El-Shaimaa A. Arafa1Hafiza Sidra Yaseen2Muhammad Ihtisham Umar3Ghulam Murtaza4College of Pharmacy and Health Sciences, Ajman University, Ajman, UAECollege of Pharmacy and Health Sciences, Ajman University, Ajman, UAEDepartment of Pharmacy, COMSATS University Islamabad, Lahore Campus, Lahore, PakistanDepartment of Pharmacy, COMSATS University Islamabad, Lahore Campus, Lahore, PakistanDepartment of Pharmacy, COMSATS University Islamabad, Lahore Campus, Lahore, PakistanAbstractPurpose The present study was carried out to evaluate anti-inflammatory and antiangiogenic attributes of simvastatin and its nanofilms containing silver nanoparticles.Methods Silver nanoparticles and simvastatin-loaded nanocomposite (SNSN) films were formulated by using polymeric solution (pectin + sericin) through casting solution method. Different in vitro and in vivo anti-inflammatory assays were performed. In addition, chick chorioallantoic membrane assay (CAM) was also employed for angiogenesis activity.Results FTIR spectra of the film depicted the presence of intact simvastatin. Differential scanning calorimetry exhibited no endothermic expression in F9 film thermogram. The simvastatin release from all films exhibited a burst effect. Cotton-pellet induced granuloma model study showed that high dose of simvastatin and indomethacin produced comparable (p < 0.05) anti-inflammatory effect. Noteworthy, RT-PCR showed dose-dependent, anti-oedematous effect of simvastatin through downregulation of serum TNF-α and interleukin-1ß levels. While results of CAM assay exhibited remarkable anti-angiogenic potential of SNSN films showing dissolved blood vessels network macroscopically.Conclusion To reiterate, simvastatin and its SNSN films can add significant contribution to the field of biomedicines due to their promising anti-inflammatory and antiangiogenic properties, however, clinical studies are required to validate their commercial use.https://www.tandfonline.com/doi/10.1080/21691401.2022.2098306TNF-alphainflammationangiogenesisinterleukinsantihyperlipidemic |
| spellingShingle | Manal Buabeid El-Shaimaa A. Arafa Hafiza Sidra Yaseen Muhammad Ihtisham Umar Ghulam Murtaza Anti-inflammatory effect of simvastatin by impeding TNF-α and interleukin-1ß pathways: antiangiogenic activity of simvastatin and simvastatin-loaded silver nanoparticles Artificial Cells, Nanomedicine, and Biotechnology TNF-alpha inflammation angiogenesis interleukins antihyperlipidemic |
| title | Anti-inflammatory effect of simvastatin by impeding TNF-α and interleukin-1ß pathways: antiangiogenic activity of simvastatin and simvastatin-loaded silver nanoparticles |
| title_full | Anti-inflammatory effect of simvastatin by impeding TNF-α and interleukin-1ß pathways: antiangiogenic activity of simvastatin and simvastatin-loaded silver nanoparticles |
| title_fullStr | Anti-inflammatory effect of simvastatin by impeding TNF-α and interleukin-1ß pathways: antiangiogenic activity of simvastatin and simvastatin-loaded silver nanoparticles |
| title_full_unstemmed | Anti-inflammatory effect of simvastatin by impeding TNF-α and interleukin-1ß pathways: antiangiogenic activity of simvastatin and simvastatin-loaded silver nanoparticles |
| title_short | Anti-inflammatory effect of simvastatin by impeding TNF-α and interleukin-1ß pathways: antiangiogenic activity of simvastatin and simvastatin-loaded silver nanoparticles |
| title_sort | anti inflammatory effect of simvastatin by impeding tnf α and interleukin 1ss pathways antiangiogenic activity of simvastatin and simvastatin loaded silver nanoparticles |
| topic | TNF-alpha inflammation angiogenesis interleukins antihyperlipidemic |
| url | https://www.tandfonline.com/doi/10.1080/21691401.2022.2098306 |
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