Heterologous Hydrogenase Overproduction Systems for Biotechnology—An Overview
Hydrogenases are complex metalloenzymes, showing tremendous potential as H<sub>2</sub>-converting redox catalysts for application in light-driven H<sub>2</sub> production, enzymatic fuel cells and H<sub>2</sub>-driven cofactor regeneration. They catalyze the rever...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-08-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/16/5890 |
| _version_ | 1797557830333497344 |
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| author | Qin Fan Peter Neubauer Oliver Lenz Matthias Gimpel |
| author_facet | Qin Fan Peter Neubauer Oliver Lenz Matthias Gimpel |
| author_sort | Qin Fan |
| collection | DOAJ |
| description | Hydrogenases are complex metalloenzymes, showing tremendous potential as H<sub>2</sub>-converting redox catalysts for application in light-driven H<sub>2</sub> production, enzymatic fuel cells and H<sub>2</sub>-driven cofactor regeneration. They catalyze the reversible oxidation of hydrogen into protons and electrons. The apo-enzymes are not active unless they are modified by a complicated post-translational maturation process that is responsible for the assembly and incorporation of the complex metal center. The catalytic center is usually easily inactivated by oxidation, and the separation and purification of the active protein is challenging. The understanding of the catalytic mechanisms progresses slowly, since the purification of the enzymes from their native hosts is often difficult, and in some case impossible. Over the past decades, only a limited number of studies report the homologous or heterologous production of high yields of hydrogenase. In this review, we emphasize recent discoveries that have greatly improved our understanding of microbial hydrogenases. We compare various heterologous hydrogenase production systems as well as in vitro hydrogenase maturation systems and discuss their perspectives for enhanced biohydrogen production. Additionally, activities of hydrogenases isolated from either recombinant organisms or in vivo/in vitro maturation approaches were systematically compared, and future perspectives for this research area are discussed. |
| first_indexed | 2024-03-10T17:21:49Z |
| format | Article |
| id | doaj.art-bc52f86ab6144f438a38e44e149dedfd |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T17:21:49Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-bc52f86ab6144f438a38e44e149dedfd2023-11-20T10:19:20ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-08-012116589010.3390/ijms21165890Heterologous Hydrogenase Overproduction Systems for Biotechnology—An OverviewQin Fan0Peter Neubauer1Oliver Lenz2Matthias Gimpel3Institute of Biotechnology, Technical University of Berlin, Ackerstraße 76, 13355 Berlin, GermanyInstitute of Biotechnology, Technical University of Berlin, Ackerstraße 76, 13355 Berlin, GermanyDepartment of Chemistry, Technical University of Berlin, Straße des 17. Juni 135, 10623 Berlin, GermanyInstitute of Biotechnology, Technical University of Berlin, Ackerstraße 76, 13355 Berlin, GermanyHydrogenases are complex metalloenzymes, showing tremendous potential as H<sub>2</sub>-converting redox catalysts for application in light-driven H<sub>2</sub> production, enzymatic fuel cells and H<sub>2</sub>-driven cofactor regeneration. They catalyze the reversible oxidation of hydrogen into protons and electrons. The apo-enzymes are not active unless they are modified by a complicated post-translational maturation process that is responsible for the assembly and incorporation of the complex metal center. The catalytic center is usually easily inactivated by oxidation, and the separation and purification of the active protein is challenging. The understanding of the catalytic mechanisms progresses slowly, since the purification of the enzymes from their native hosts is often difficult, and in some case impossible. Over the past decades, only a limited number of studies report the homologous or heterologous production of high yields of hydrogenase. In this review, we emphasize recent discoveries that have greatly improved our understanding of microbial hydrogenases. We compare various heterologous hydrogenase production systems as well as in vitro hydrogenase maturation systems and discuss their perspectives for enhanced biohydrogen production. Additionally, activities of hydrogenases isolated from either recombinant organisms or in vivo/in vitro maturation approaches were systematically compared, and future perspectives for this research area are discussed.https://www.mdpi.com/1422-0067/21/16/5890hydrogenasesheterologous expression systemsmetalloproteinbiohydrogenrenewable energyoxygen-tolerance |
| spellingShingle | Qin Fan Peter Neubauer Oliver Lenz Matthias Gimpel Heterologous Hydrogenase Overproduction Systems for Biotechnology—An Overview International Journal of Molecular Sciences hydrogenases heterologous expression systems metalloprotein biohydrogen renewable energy oxygen-tolerance |
| title | Heterologous Hydrogenase Overproduction Systems for Biotechnology—An Overview |
| title_full | Heterologous Hydrogenase Overproduction Systems for Biotechnology—An Overview |
| title_fullStr | Heterologous Hydrogenase Overproduction Systems for Biotechnology—An Overview |
| title_full_unstemmed | Heterologous Hydrogenase Overproduction Systems for Biotechnology—An Overview |
| title_short | Heterologous Hydrogenase Overproduction Systems for Biotechnology—An Overview |
| title_sort | heterologous hydrogenase overproduction systems for biotechnology an overview |
| topic | hydrogenases heterologous expression systems metalloprotein biohydrogen renewable energy oxygen-tolerance |
| url | https://www.mdpi.com/1422-0067/21/16/5890 |
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