Palmitoleate Protects against Zika Virus-Induced Placental Trophoblast Apoptosis
Zika virus (ZIKV) infection in pregnancy is associated with the development of microcephaly, intrauterine growth restriction, and ocular damage in the fetus. ZIKV infection of the placenta plays a crucial role in the vertical transmission from the maternal circulation to the fetus. Our previous stud...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-06-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/9/6/643 |
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| author | Philma Glora Muthuraj Aryamav Pattnaik Prakash K. Sahoo Md Torikul Islam Asit K. Pattnaik Siddappa N. Byrareddy Corrine Hanson Ann Anderson Berry Stephen D. Kachman Sathish Kumar Natarajan |
| author_facet | Philma Glora Muthuraj Aryamav Pattnaik Prakash K. Sahoo Md Torikul Islam Asit K. Pattnaik Siddappa N. Byrareddy Corrine Hanson Ann Anderson Berry Stephen D. Kachman Sathish Kumar Natarajan |
| author_sort | Philma Glora Muthuraj |
| collection | DOAJ |
| description | Zika virus (ZIKV) infection in pregnancy is associated with the development of microcephaly, intrauterine growth restriction, and ocular damage in the fetus. ZIKV infection of the placenta plays a crucial role in the vertical transmission from the maternal circulation to the fetus. Our previous study suggested that ZIKV induces endoplasmic reticulum (ER) stress and apoptosis of placental trophoblasts. Here, we showed that palmitoleate, an omega-7 monounsaturated fatty acid, prevents ZIKV-induced ER stress and apoptosis in placental trophoblasts. Human trophoblast cell lines (JEG-3 and JAR) and normal immortalized trophoblasts (HTR-8) were used. We observed that ZIKV infection of the trophoblasts resulted in apoptosis and treatment of palmitoleate to ZIKV-infected cells significantly prevented apoptosis. However, palmitate (saturated fatty acid) did not offer protection from ZIKV-induced ER stress and apoptosis. We also observed that the Zika viral RNA copies were decreased, and the cell viability improved in ZIKV-infected cells treated with palmitoleate as compared to the infected cells without palmitoleate treatment. Further, palmitoleate was shown to protect against ZIKV-induced upregulation of ER stress markers, C/EBP homologous protein and X-box binding protein-1 splicing in placental trophoblasts. In conclusion, our studies suggest that palmitoleate protects placental trophoblasts against ZIKV-induced ER stress and apoptosis. |
| first_indexed | 2024-03-10T10:42:18Z |
| format | Article |
| id | doaj.art-bc532a1c34e445f5beb6f8063cea2157 |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-10T10:42:18Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-bc532a1c34e445f5beb6f8063cea21572023-11-21T22:53:27ZengMDPI AGBiomedicines2227-90592021-06-019664310.3390/biomedicines9060643Palmitoleate Protects against Zika Virus-Induced Placental Trophoblast ApoptosisPhilma Glora Muthuraj0Aryamav Pattnaik1Prakash K. Sahoo2Md Torikul Islam3Asit K. Pattnaik4Siddappa N. Byrareddy5Corrine Hanson6Ann Anderson Berry7Stephen D. Kachman8Sathish Kumar Natarajan9Department of Nutrition and Health Sciences, University of Nebraska-Lincoln, Lincoln, NE 68583, USANebraska Center for Virology, University of Nebraska-Lincoln, Lincoln, NE 68583, USADepartment of Nutrition and Health Sciences, University of Nebraska-Lincoln, Lincoln, NE 68583, USADepartment of Nutrition and Health Sciences, University of Nebraska-Lincoln, Lincoln, NE 68583, USANebraska Center for Virology, University of Nebraska-Lincoln, Lincoln, NE 68583, USADepartment of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, NE 68198, USAChild Health Research Institute, University of Nebraska Medical Center, Omaha, NE 68198, USAChild Health Research Institute, University of Nebraska Medical Center, Omaha, NE 68198, USADepartment of Statistics, University of Nebraska-Lincoln, Lincoln, NE 68583, USADepartment of Nutrition and Health Sciences, University of Nebraska-Lincoln, Lincoln, NE 68583, USAZika virus (ZIKV) infection in pregnancy is associated with the development of microcephaly, intrauterine growth restriction, and ocular damage in the fetus. ZIKV infection of the placenta plays a crucial role in the vertical transmission from the maternal circulation to the fetus. Our previous study suggested that ZIKV induces endoplasmic reticulum (ER) stress and apoptosis of placental trophoblasts. Here, we showed that palmitoleate, an omega-7 monounsaturated fatty acid, prevents ZIKV-induced ER stress and apoptosis in placental trophoblasts. Human trophoblast cell lines (JEG-3 and JAR) and normal immortalized trophoblasts (HTR-8) were used. We observed that ZIKV infection of the trophoblasts resulted in apoptosis and treatment of palmitoleate to ZIKV-infected cells significantly prevented apoptosis. However, palmitate (saturated fatty acid) did not offer protection from ZIKV-induced ER stress and apoptosis. We also observed that the Zika viral RNA copies were decreased, and the cell viability improved in ZIKV-infected cells treated with palmitoleate as compared to the infected cells without palmitoleate treatment. Further, palmitoleate was shown to protect against ZIKV-induced upregulation of ER stress markers, C/EBP homologous protein and X-box binding protein-1 splicing in placental trophoblasts. In conclusion, our studies suggest that palmitoleate protects placental trophoblasts against ZIKV-induced ER stress and apoptosis.https://www.mdpi.com/2227-9059/9/6/643monounsaturated fatty acidsendoplasmic reticulum stressapoptosisviral replicationZika virusplacenta |
| spellingShingle | Philma Glora Muthuraj Aryamav Pattnaik Prakash K. Sahoo Md Torikul Islam Asit K. Pattnaik Siddappa N. Byrareddy Corrine Hanson Ann Anderson Berry Stephen D. Kachman Sathish Kumar Natarajan Palmitoleate Protects against Zika Virus-Induced Placental Trophoblast Apoptosis Biomedicines monounsaturated fatty acids endoplasmic reticulum stress apoptosis viral replication Zika virus placenta |
| title | Palmitoleate Protects against Zika Virus-Induced Placental Trophoblast Apoptosis |
| title_full | Palmitoleate Protects against Zika Virus-Induced Placental Trophoblast Apoptosis |
| title_fullStr | Palmitoleate Protects against Zika Virus-Induced Placental Trophoblast Apoptosis |
| title_full_unstemmed | Palmitoleate Protects against Zika Virus-Induced Placental Trophoblast Apoptosis |
| title_short | Palmitoleate Protects against Zika Virus-Induced Placental Trophoblast Apoptosis |
| title_sort | palmitoleate protects against zika virus induced placental trophoblast apoptosis |
| topic | monounsaturated fatty acids endoplasmic reticulum stress apoptosis viral replication Zika virus placenta |
| url | https://www.mdpi.com/2227-9059/9/6/643 |
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