<italic toggle="yes">In Vivo</italic> Thermodynamic Analysis of Glycolysis in <named-content content-type="genus-species">Clostridium thermocellum</named-content> and <named-content content-type="genus-species">Thermoanaerobacterium saccharolyticum</named-content> Using <sup>13</sup>C and <sup>2</sup>H Tracers

<italic toggle="yes">In Vivo</italic> Thermodynamic Analysis of Glycolysis in <named-content content-type="genus-species">Clostridium thermocellum</named-content> and <named-content content-type="genus-species">Thermoanaerobacterium saccharolyticum</named-content> Using <sup>13</sup>C and <sup>2</sup>H Tracers

ABSTRACT Clostridium thermocellum and Thermoanaerobacterium saccharolyticum are thermophilic anaerobic bacteria with complementary metabolic capabilities that utilize distinct glycolytic pathways for the conversion of cellulosic sugars to biofuels. We integrated quantitative metabolomics with 2H and...

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Bibliographic Details
Main Authors: Tyler B. Jacobson, Travis K. Korosh, David M. Stevenson, Charles Foster, Costas Maranas, Daniel G. Olson, Lee R. Lynd, Daniel Amador-Noguez
Format: Article
Language:English
Published: American Society for Microbiology 2020-04-01
Series:mSystems
Subjects:
microbial metabolism
metabolic flux analysis
MFA
mass spectrometry
biofuels
Gibbs free energy
Online Access:https://journals.asm.org/doi/10.1128/mSystems.00736-19

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