Cellulosic Fiber Waste Feedstock for Bioethanol Production via Bioreactor-Dependent Fermentation
The bioconversion of environmental wastes into energy is gaining much interest in most developing and developed countries. The current study is concerned with the proper exploitation of some industrial wastes. Cellulosic fiber waste was selected as a raw material for producing bioethanol as an alter...
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MDPI AG
2023-02-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/9/2/176 |
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| author | Ahmed E. Mansy Eman El-Desouky Hamada El-Gendi Mohamed A. Abu-Saied Tarek H. Taha Ranya A. Amer |
| author_facet | Ahmed E. Mansy Eman El-Desouky Hamada El-Gendi Mohamed A. Abu-Saied Tarek H. Taha Ranya A. Amer |
| author_sort | Ahmed E. Mansy |
| collection | DOAJ |
| description | The bioconversion of environmental wastes into energy is gaining much interest in most developing and developed countries. The current study is concerned with the proper exploitation of some industrial wastes. Cellulosic fiber waste was selected as a raw material for producing bioethanol as an alternative energy source. A combination of physical, chemical, and enzymatic hydrolysis treatments was applied to maximize the concentration of glucose that could be fermented with yeast into bioethanol. The results showed that the maximum production of 13.9 mg/mL of glucose was achieved when 5% cellulosic fiber waste was treated with 40% HCl, autoclaved, and followed with enzymatic hydrolysis. Using SEM and FTIR analysis, the instrumental characterization of the waste fiber treatment confirmed the effectiveness of the degradation by turning the long threads of the fibers into small pieces, in addition to the appearance of new functional groups and peak shifting. A potent yeast strain isolated from rotten grapes was identified as <i>Starmerella bacillaris</i> STDF-G4 (accession number OP872748), which was used to ferment the obtained glucose units into bioethanol under optimized conditions. The maximum production of 3.16 mg/mL of bioethanol was recorded when 7% of the yeast strain was anaerobically incubated at 30 °C in a broth culture with the pH adjusted to 5. The optimized conditions were scaled up from flasks to a fermentation bioreactor to maximize the bioethanol concentration. The obtained data showed the ability of the yeast strain to produce 4.13 mg/mL of bioethanol after the first 6 h of incubation and double the amount after 36 h of incubation to reach 8.6 mg/mL, indicating the efficiency of the bioreactor in reducing the time and significantly increasing the product. |
| first_indexed | 2024-03-11T08:51:09Z |
| format | Article |
| id | doaj.art-bc5654d91a8242d2a88906e6f216fd63 |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-11T08:51:09Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-bc5654d91a8242d2a88906e6f216fd632023-11-16T20:26:32ZengMDPI AGFermentation2311-56372023-02-019217610.3390/fermentation9020176Cellulosic Fiber Waste Feedstock for Bioethanol Production via Bioreactor-Dependent FermentationAhmed E. Mansy0Eman El-Desouky1Hamada El-Gendi2Mohamed A. Abu-Saied3Tarek H. Taha4Ranya A. Amer5Environment and Natural Materials Research Institute (ENMRI), City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab City 21934, Alexandria, EgyptChemistry Department, Faculty of Science, Alexandria University, Ibrahimia, Alexandria 21321, Alexandria, EgyptBioprocess Development Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab City 21934, Alexandria, EgyptPolymeric Materials Research Department, Advanced Technology and New Materials Research Institute (ATNMRI), City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab City 21934, Alexandria, EgyptEnvironmental Biotechnology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab City 21934, Alexandria, EgyptEnvironmental Biotechnology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab City 21934, Alexandria, EgyptThe bioconversion of environmental wastes into energy is gaining much interest in most developing and developed countries. The current study is concerned with the proper exploitation of some industrial wastes. Cellulosic fiber waste was selected as a raw material for producing bioethanol as an alternative energy source. A combination of physical, chemical, and enzymatic hydrolysis treatments was applied to maximize the concentration of glucose that could be fermented with yeast into bioethanol. The results showed that the maximum production of 13.9 mg/mL of glucose was achieved when 5% cellulosic fiber waste was treated with 40% HCl, autoclaved, and followed with enzymatic hydrolysis. Using SEM and FTIR analysis, the instrumental characterization of the waste fiber treatment confirmed the effectiveness of the degradation by turning the long threads of the fibers into small pieces, in addition to the appearance of new functional groups and peak shifting. A potent yeast strain isolated from rotten grapes was identified as <i>Starmerella bacillaris</i> STDF-G4 (accession number OP872748), which was used to ferment the obtained glucose units into bioethanol under optimized conditions. The maximum production of 3.16 mg/mL of bioethanol was recorded when 7% of the yeast strain was anaerobically incubated at 30 °C in a broth culture with the pH adjusted to 5. The optimized conditions were scaled up from flasks to a fermentation bioreactor to maximize the bioethanol concentration. The obtained data showed the ability of the yeast strain to produce 4.13 mg/mL of bioethanol after the first 6 h of incubation and double the amount after 36 h of incubation to reach 8.6 mg/mL, indicating the efficiency of the bioreactor in reducing the time and significantly increasing the product.https://www.mdpi.com/2311-5637/9/2/176bioethanolbioreactor fermentationcellulosic fiber wasteenzymatic hydrolysisphysico-chemical treatments |
| spellingShingle | Ahmed E. Mansy Eman El-Desouky Hamada El-Gendi Mohamed A. Abu-Saied Tarek H. Taha Ranya A. Amer Cellulosic Fiber Waste Feedstock for Bioethanol Production via Bioreactor-Dependent Fermentation Fermentation bioethanol bioreactor fermentation cellulosic fiber waste enzymatic hydrolysis physico-chemical treatments |
| title | Cellulosic Fiber Waste Feedstock for Bioethanol Production via Bioreactor-Dependent Fermentation |
| title_full | Cellulosic Fiber Waste Feedstock for Bioethanol Production via Bioreactor-Dependent Fermentation |
| title_fullStr | Cellulosic Fiber Waste Feedstock for Bioethanol Production via Bioreactor-Dependent Fermentation |
| title_full_unstemmed | Cellulosic Fiber Waste Feedstock for Bioethanol Production via Bioreactor-Dependent Fermentation |
| title_short | Cellulosic Fiber Waste Feedstock for Bioethanol Production via Bioreactor-Dependent Fermentation |
| title_sort | cellulosic fiber waste feedstock for bioethanol production via bioreactor dependent fermentation |
| topic | bioethanol bioreactor fermentation cellulosic fiber waste enzymatic hydrolysis physico-chemical treatments |
| url | https://www.mdpi.com/2311-5637/9/2/176 |
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