Single antibody detection in a DNA origami nanoantenna
Summary: DNA nanotechnology offers new biosensing approaches by templating different sensor and transducer components. Here, we combine DNA origami nanoantennas with label-free antibody detection by incorporating a nanoswitch in the plasmonic hotspot of the nanoantenna. The nanoswitch contains two a...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2021-09-01
|
| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004221010403 |
| _version_ | 1819134652729786368 |
|---|---|
| author | Martina Pfeiffer Kateryna Trofymchuk Simona Ranallo Francesco Ricci Florian Steiner Fiona Cole Viktorija Glembockyte Philip Tinnefeld |
| author_facet | Martina Pfeiffer Kateryna Trofymchuk Simona Ranallo Francesco Ricci Florian Steiner Fiona Cole Viktorija Glembockyte Philip Tinnefeld |
| author_sort | Martina Pfeiffer |
| collection | DOAJ |
| description | Summary: DNA nanotechnology offers new biosensing approaches by templating different sensor and transducer components. Here, we combine DNA origami nanoantennas with label-free antibody detection by incorporating a nanoswitch in the plasmonic hotspot of the nanoantenna. The nanoswitch contains two antigens that are displaced by antibody binding, thereby eliciting a fluorescent signal. Single-antibody detection is demonstrated with a DNA origami integrated anti-digoxigenin antibody nanoswitch. In combination with the nanoantenna, the signal generated by the antibody is additionally amplified. This allows the detection of single antibodies on a portable smartphone microscope. Overall, fluorescence-enhanced antibody detection in DNA origami nanoantennas shows that fluorescence-enhanced biosensing can be expanded beyond the scope of the nucleic acids realm. |
| first_indexed | 2024-12-22T10:06:36Z |
| format | Article |
| id | doaj.art-bc56764a874446e9ae707e4cb93a0723 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-12-22T10:06:36Z |
| publishDate | 2021-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-bc56764a874446e9ae707e4cb93a07232022-12-21T18:29:57ZengElsevieriScience2589-00422021-09-01249103072Single antibody detection in a DNA origami nanoantennaMartina Pfeiffer0Kateryna Trofymchuk1Simona Ranallo2Francesco Ricci3Florian Steiner4Fiona Cole5Viktorija Glembockyte6Philip Tinnefeld7Department of Chemistry and Center for NanoScience, Ludwig-Maximilians-Universität München, Butenandtstr. 5-13, 81377 München, GermanyDepartment of Chemistry and Center for NanoScience, Ludwig-Maximilians-Universität München, Butenandtstr. 5-13, 81377 München, GermanyDepartment of Chemical Science and Technologies, University of Rome, Tor Vergata, Via della Ricerca Scientifica 1, 00133 Rome, Italy; Department of Chemistry and Biochemistry, University of California Santa Barbara, Santa Barbara, CA 93106, USADepartment of Chemical Science and Technologies, University of Rome, Tor Vergata, Via della Ricerca Scientifica 1, 00133 Rome, ItalyDepartment of Chemistry and Center for NanoScience, Ludwig-Maximilians-Universität München, Butenandtstr. 5-13, 81377 München, GermanyDepartment of Chemistry and Center for NanoScience, Ludwig-Maximilians-Universität München, Butenandtstr. 5-13, 81377 München, GermanyDepartment of Chemistry and Center for NanoScience, Ludwig-Maximilians-Universität München, Butenandtstr. 5-13, 81377 München, Germany; Corresponding authorDepartment of Chemistry and Center for NanoScience, Ludwig-Maximilians-Universität München, Butenandtstr. 5-13, 81377 München, Germany; Corresponding authorSummary: DNA nanotechnology offers new biosensing approaches by templating different sensor and transducer components. Here, we combine DNA origami nanoantennas with label-free antibody detection by incorporating a nanoswitch in the plasmonic hotspot of the nanoantenna. The nanoswitch contains two antigens that are displaced by antibody binding, thereby eliciting a fluorescent signal. Single-antibody detection is demonstrated with a DNA origami integrated anti-digoxigenin antibody nanoswitch. In combination with the nanoantenna, the signal generated by the antibody is additionally amplified. This allows the detection of single antibodies on a portable smartphone microscope. Overall, fluorescence-enhanced antibody detection in DNA origami nanoantennas shows that fluorescence-enhanced biosensing can be expanded beyond the scope of the nucleic acids realm.http://www.sciencedirect.com/science/article/pii/S2589004221010403Physical chemistryOpticsNanotechnologyBiochemistry |
| spellingShingle | Martina Pfeiffer Kateryna Trofymchuk Simona Ranallo Francesco Ricci Florian Steiner Fiona Cole Viktorija Glembockyte Philip Tinnefeld Single antibody detection in a DNA origami nanoantenna iScience Physical chemistry Optics Nanotechnology Biochemistry |
| title | Single antibody detection in a DNA origami nanoantenna |
| title_full | Single antibody detection in a DNA origami nanoantenna |
| title_fullStr | Single antibody detection in a DNA origami nanoantenna |
| title_full_unstemmed | Single antibody detection in a DNA origami nanoantenna |
| title_short | Single antibody detection in a DNA origami nanoantenna |
| title_sort | single antibody detection in a dna origami nanoantenna |
| topic | Physical chemistry Optics Nanotechnology Biochemistry |
| url | http://www.sciencedirect.com/science/article/pii/S2589004221010403 |
| work_keys_str_mv | AT martinapfeiffer singleantibodydetectioninadnaorigaminanoantenna AT katerynatrofymchuk singleantibodydetectioninadnaorigaminanoantenna AT simonaranallo singleantibodydetectioninadnaorigaminanoantenna AT francescoricci singleantibodydetectioninadnaorigaminanoantenna AT floriansteiner singleantibodydetectioninadnaorigaminanoantenna AT fionacole singleantibodydetectioninadnaorigaminanoantenna AT viktorijaglembockyte singleantibodydetectioninadnaorigaminanoantenna AT philiptinnefeld singleantibodydetectioninadnaorigaminanoantenna |