Galanginin Protects AML-12 Cells Against Dactinomycin Induced Hepatotoxicity
Aim:The purpose of this study was to evaluate the effects of galanginin (Gal) on dactinomycin induced hepatotoxicity in vitro.Materials and Methods:AML-12 cell line was divided into 4 groups as the control, Gal, dactinomycin, and Gal+dactinomycin groups. IC50 dose was determined by the thiazolyl blu...
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| Format: | Article |
| Language: | English |
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Galenos Yayincilik
2023-09-01
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| Series: | Namık Kemal Tıp Dergisi |
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http://namikkemalmedj.com/archives/archive-detail/article-preview/galanginin-protects-aml-12-cells-against-dactinomy/62103
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| author | Melek AKINCI Çağatay OLTULU Elvan BAKAR Zatiye Ayça ÇEVİKELLİ YAKUT |
| author_facet | Melek AKINCI Çağatay OLTULU Elvan BAKAR Zatiye Ayça ÇEVİKELLİ YAKUT |
| author_sort | Melek AKINCI |
| collection | DOAJ |
| description | Aim:The purpose of this study was to evaluate the effects of galanginin (Gal) on dactinomycin induced hepatotoxicity in vitro.Materials and Methods:AML-12 cell line was divided into 4 groups as the control, Gal, dactinomycin, and Gal+dactinomycin groups. IC50 dose was determined by the thiazolyl blue tetrazolium bromide test. Gene expressions of glutathione (GSH), superoxide dismutase (SOD), catalase, caspase 3 (Cas-3), Cas-9, apoptotic protease activating factor-1 (Apaf-1), B cell CLL/lymphoma-2 (Bcl-2), Bcl-2 associated X protein (Bax), tumor protein p53 (p53), second mitochondria-derived activator of caspase/direct inhibitor of apoptosis-binding protein (smac/DIABLO), topoisomerase (Top) I, and Top II were determined with quantitative real-time polymerase chain reaction analysis.Results:Dactinomycin elevated the expression of SOD, catalase, and GSH in response to oxidative effects. In the Gal+dactinomycin group, Gal administration reduced Apaf-1 expression and increased Bcl-2 expression with antiapoptotic effects. In the dactinomycin group, p53 levels increased due to the defense mechanism against DNA damage. Gal increased smac/DIABLO expression to remove damaged structures. Bcl-2 and smac/DIABLO expression levels in the groups were inversely proportional. In the Gal+dactinomycin group, Top II expression level was lower than in the dactinomycin group. This result indicated that double strand of DNA damage was diminished by Gal.Conclusion:Gal protected against the hepatotoxicity due to dactinomycin with antioxidant and antiapoptotic effects. Further experimental studies are needed to establish the use of Gal in liver damage. |
| first_indexed | 2024-03-12T00:23:23Z |
| format | Article |
| id | doaj.art-bc59d7c8ce5b49c7b74fcaad48c6a4e2 |
| institution | Directory Open Access Journal |
| issn | 2587-0262 |
| language | English |
| last_indexed | 2024-03-12T00:23:23Z |
| publishDate | 2023-09-01 |
| publisher | Galenos Yayincilik |
| record_format | Article |
| series | Namık Kemal Tıp Dergisi |
| spelling | doaj.art-bc59d7c8ce5b49c7b74fcaad48c6a4e22023-09-15T11:08:39ZengGalenos YayincilikNamık Kemal Tıp Dergisi2587-02622023-09-0111325726410.4274/nkmj.galenos.2023.0922613049054Galanginin Protects AML-12 Cells Against Dactinomycin Induced HepatotoxicityMelek AKINCI0Çağatay OLTULU1Elvan BAKAR2Zatiye Ayça ÇEVİKELLİ YAKUT3 Trakya Üniversitesi Eczacılık Fakültesi, Farmakoloji Anabilim Dalı, Edirne, Türkiye Trakya Üniversitesi Eczacılık Fakültesi, Farmasötik Toksikoloji Anabilim Dalı, Edirne, Türkiye Trakya Üniversitesi Eczacılık Fakültesi, Temel Bilimler Anabilim Dalı, Edirne, Türkiye Trakya Üniversitesi Eczacılık Fakültesi, Farmakognozi Anabilim Dalı, Edirne; Marmara Üniversitesi Eczacılık Fakültesi, Farmakoloji Anabilim Dalı, İstanbul, Türkiye Aim:The purpose of this study was to evaluate the effects of galanginin (Gal) on dactinomycin induced hepatotoxicity in vitro.Materials and Methods:AML-12 cell line was divided into 4 groups as the control, Gal, dactinomycin, and Gal+dactinomycin groups. IC50 dose was determined by the thiazolyl blue tetrazolium bromide test. Gene expressions of glutathione (GSH), superoxide dismutase (SOD), catalase, caspase 3 (Cas-3), Cas-9, apoptotic protease activating factor-1 (Apaf-1), B cell CLL/lymphoma-2 (Bcl-2), Bcl-2 associated X protein (Bax), tumor protein p53 (p53), second mitochondria-derived activator of caspase/direct inhibitor of apoptosis-binding protein (smac/DIABLO), topoisomerase (Top) I, and Top II were determined with quantitative real-time polymerase chain reaction analysis.Results:Dactinomycin elevated the expression of SOD, catalase, and GSH in response to oxidative effects. In the Gal+dactinomycin group, Gal administration reduced Apaf-1 expression and increased Bcl-2 expression with antiapoptotic effects. In the dactinomycin group, p53 levels increased due to the defense mechanism against DNA damage. Gal increased smac/DIABLO expression to remove damaged structures. Bcl-2 and smac/DIABLO expression levels in the groups were inversely proportional. In the Gal+dactinomycin group, Top II expression level was lower than in the dactinomycin group. This result indicated that double strand of DNA damage was diminished by Gal.Conclusion:Gal protected against the hepatotoxicity due to dactinomycin with antioxidant and antiapoptotic effects. Further experimental studies are needed to establish the use of Gal in liver damage. http://namikkemalmedj.com/archives/archive-detail/article-preview/galanginin-protects-aml-12-cells-against-dactinomy/62103 dactinomycingalanginaml-12 cell linehepatotoxicityoxidative stressapoptosis |
| spellingShingle | Melek AKINCI Çağatay OLTULU Elvan BAKAR Zatiye Ayça ÇEVİKELLİ YAKUT Galanginin Protects AML-12 Cells Against Dactinomycin Induced Hepatotoxicity Namık Kemal Tıp Dergisi dactinomycin galangin aml-12 cell line hepatotoxicity oxidative stress apoptosis |
| title | Galanginin Protects AML-12 Cells Against Dactinomycin Induced Hepatotoxicity |
| title_full | Galanginin Protects AML-12 Cells Against Dactinomycin Induced Hepatotoxicity |
| title_fullStr | Galanginin Protects AML-12 Cells Against Dactinomycin Induced Hepatotoxicity |
| title_full_unstemmed | Galanginin Protects AML-12 Cells Against Dactinomycin Induced Hepatotoxicity |
| title_short | Galanginin Protects AML-12 Cells Against Dactinomycin Induced Hepatotoxicity |
| title_sort | galanginin protects aml 12 cells against dactinomycin induced hepatotoxicity |
| topic | dactinomycin galangin aml-12 cell line hepatotoxicity oxidative stress apoptosis |
| url |
http://namikkemalmedj.com/archives/archive-detail/article-preview/galanginin-protects-aml-12-cells-against-dactinomy/62103
|
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