Bioactive Composition, Antioxidant, And Cytotoxic Activities of Rheum Ribes Extracts
The aim of the study is to evaluate the ethanol extract of Rheum ribes root (RTE), as well as the root and young shoots (RYSE), for its chemical composition, antioxidant properties and cytotoxic effects. Total polyphenols (TPC), total flavonoid (TFC), radical scavenging activity (DPPH), the ferric r...
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| Format: | Article |
| Language: | English |
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Turkish Science and Technology Publishing (TURSTEP)
2023-08-01
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| Series: | Turkish Journal of Agriculture: Food Science and Technology |
| Subjects: | |
| Online Access: | http://www.agrifoodscience.com/index.php/TURJAF/article/view/6023 |
| _version_ | 1797671553829175296 |
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| author | Sema Mısır Mahmut Özbek Ceylan Hepokur |
| author_facet | Sema Mısır Mahmut Özbek Ceylan Hepokur |
| author_sort | Sema Mısır |
| collection | DOAJ |
| description | The aim of the study is to evaluate the ethanol extract of Rheum ribes root (RTE), as well as the root and young shoots (RYSE), for its chemical composition, antioxidant properties and cytotoxic effects. Total polyphenols (TPC), total flavonoid (TFC), radical scavenging activity (DPPH), the ferric reducing antioxidant power (FRAP) of Rheum ribes extracts were determined using colorimetric methods. Mineral contents and vitamin values of samples were determined by ICP-MS and HPLC, respectively. The cytotoxic effect of Rheum ribes extracts was determined on different cells using XTT assay. The cytotoxic effects of RTE and RYSE on cancer cells were evaluated with regard to apoptosis. According to results, mineral contents, vitamine A and C values were very high in both samples. Ethanolic extracts of Rheum ribes young shoot exhibited a selective cytotoxic effect on all cancer cells compared to WI-38 cells, and the IC50 values of the extract in the cancer cells between 26.10 to 54.81 μg/mL. Ethanolic extracts of RTE and RYSE induced apoptosis on MCF-7 cells. The ethanolic extracts of Rheum ribes has effective cytotoxic and antioxidant activity. More research is needed to determine the cytotoxic effect mechanisms on cancer cells. |
| first_indexed | 2024-03-11T21:17:14Z |
| format | Article |
| id | doaj.art-bc5b2195c86340da817266bb6146ce6d |
| institution | Directory Open Access Journal |
| issn | 2148-127X |
| language | English |
| last_indexed | 2024-03-11T21:17:14Z |
| publishDate | 2023-08-01 |
| publisher | Turkish Science and Technology Publishing (TURSTEP) |
| record_format | Article |
| series | Turkish Journal of Agriculture: Food Science and Technology |
| spelling | doaj.art-bc5b2195c86340da817266bb6146ce6d2023-09-28T16:05:13ZengTurkish Science and Technology Publishing (TURSTEP)Turkish Journal of Agriculture: Food Science and Technology2148-127X2023-08-011181345135010.24925/turjaf.v11i8.1345-1350.60234724Bioactive Composition, Antioxidant, And Cytotoxic Activities of Rheum Ribes ExtractsSema Mısır0https://orcid.org/0000-0002-5919-3295Mahmut Özbek1https://orcid.org/0000-0002-8126-2293Ceylan Hepokur2https://orcid.org/0000-0001-6397-1291Sivas Cumhuriyet University, Faculty of Pharmacy, Department of Biochemistry, Sivas, TurkeyWorld Medicine Pharmaceutical Companysivas cumhuriyet üniversitesiThe aim of the study is to evaluate the ethanol extract of Rheum ribes root (RTE), as well as the root and young shoots (RYSE), for its chemical composition, antioxidant properties and cytotoxic effects. Total polyphenols (TPC), total flavonoid (TFC), radical scavenging activity (DPPH), the ferric reducing antioxidant power (FRAP) of Rheum ribes extracts were determined using colorimetric methods. Mineral contents and vitamin values of samples were determined by ICP-MS and HPLC, respectively. The cytotoxic effect of Rheum ribes extracts was determined on different cells using XTT assay. The cytotoxic effects of RTE and RYSE on cancer cells were evaluated with regard to apoptosis. According to results, mineral contents, vitamine A and C values were very high in both samples. Ethanolic extracts of Rheum ribes young shoot exhibited a selective cytotoxic effect on all cancer cells compared to WI-38 cells, and the IC50 values of the extract in the cancer cells between 26.10 to 54.81 μg/mL. Ethanolic extracts of RTE and RYSE induced apoptosis on MCF-7 cells. The ethanolic extracts of Rheum ribes has effective cytotoxic and antioxidant activity. More research is needed to determine the cytotoxic effect mechanisms on cancer cells.http://www.agrifoodscience.com/index.php/TURJAF/article/view/6023antioxidant acitivtycytotoxicityhplcicp-ms |
| spellingShingle | Sema Mısır Mahmut Özbek Ceylan Hepokur Bioactive Composition, Antioxidant, And Cytotoxic Activities of Rheum Ribes Extracts Turkish Journal of Agriculture: Food Science and Technology antioxidant acitivty cytotoxicity hplc icp-ms |
| title | Bioactive Composition, Antioxidant, And Cytotoxic Activities of Rheum Ribes Extracts |
| title_full | Bioactive Composition, Antioxidant, And Cytotoxic Activities of Rheum Ribes Extracts |
| title_fullStr | Bioactive Composition, Antioxidant, And Cytotoxic Activities of Rheum Ribes Extracts |
| title_full_unstemmed | Bioactive Composition, Antioxidant, And Cytotoxic Activities of Rheum Ribes Extracts |
| title_short | Bioactive Composition, Antioxidant, And Cytotoxic Activities of Rheum Ribes Extracts |
| title_sort | bioactive composition antioxidant and cytotoxic activities of rheum ribes extracts |
| topic | antioxidant acitivty cytotoxicity hplc icp-ms |
| url | http://www.agrifoodscience.com/index.php/TURJAF/article/view/6023 |
| work_keys_str_mv | AT semamısır bioactivecompositionantioxidantandcytotoxicactivitiesofrheumribesextracts AT mahmutozbek bioactivecompositionantioxidantandcytotoxicactivitiesofrheumribesextracts AT ceylanhepokur bioactivecompositionantioxidantandcytotoxicactivitiesofrheumribesextracts |