Changes in corneal innervation and pain responses in fungal keratitis
AIM: To characterize changes in the cornea nerve and pain responses in fungal keratitis (FK). METHODS: A retrospective analysis of in vivo confocal microscopy images of 11 FK corneas was performed, and the results were compared with those for 11 normal corneas. Subbasal corneal nerves were analyzed...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Press of International Journal of Ophthalmology (IJO PRESS)
2020-01-01
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| Series: | International Journal of Ophthalmology |
| Subjects: | |
| Online Access: | http://www.ijo.cn/en_publish/2020/1/20200101.pdf |
| _version_ | 1828475044735483904 |
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| author | Chang-Jie Ren Yi-Fan Zhou Yuan Wu Xu-Dong Peng Cui Li Qian Wang Guo-Qiang Zhu Jia You Jie Zhang Gui-Qiu Zhao Jing Lin |
| author_facet | Chang-Jie Ren Yi-Fan Zhou Yuan Wu Xu-Dong Peng Cui Li Qian Wang Guo-Qiang Zhu Jia You Jie Zhang Gui-Qiu Zhao Jing Lin |
| author_sort | Chang-Jie Ren |
| collection | DOAJ |
| description | AIM: To characterize changes in the cornea nerve and pain responses in fungal keratitis (FK).
METHODS: A retrospective analysis of in vivo confocal microscopy images of 11 FK corneas was performed, and the results were compared with those for 11 normal corneas. Subbasal corneal nerves were analyzed for total nerve number, main nerve trunk number, branching patterns and tortuosity. C57BL/6 mice were infected with Aspergillus fumigatus. Disease severity was determined through clinical scoring and slit lamp photography. Corneas were harvested at 1, 3, 5, and 7d post infection (p.i.) and assessed for β III tubulin. Corneal mechanical sensitivity thresholds were detected by von Frey test. β-endorphin (β-EP) and μ receptor protein expression was detected through Western blotting.
RESULTS: Total nerve number, main nerve trunk number, and nerve branching were significantly lower in FK patients than in controls, but tortuosity was not significantly different. In infected mice, subbasal nerve density decreased from 1d p.i., reaching a minimum at 5d p.i. Clinical scores rose at 1d p.i., peaked at 3d p.i., and decreased at 5d p.i. Mechanical sensitivity thresholds showed the same trends. β-EP and μ receptor protein expression increased after infection.
CONCLUSION: Corneal nerve density is lower in FK patients and Aspergillus fumigatus-infected mice than in controls. Pain sensitivity decreases with postinfection corneal ulcer aggravation. β-EP and μ receptor proteins are both upregulated in infected mouse corneas. |
| first_indexed | 2024-12-11T06:07:11Z |
| format | Article |
| id | doaj.art-bc5c60ae579e42f787dbf14e397d5530 |
| institution | Directory Open Access Journal |
| issn | 2222-3959 2227-4898 |
| language | English |
| last_indexed | 2024-12-11T06:07:11Z |
| publishDate | 2020-01-01 |
| publisher | Press of International Journal of Ophthalmology (IJO PRESS) |
| record_format | Article |
| series | International Journal of Ophthalmology |
| spelling | doaj.art-bc5c60ae579e42f787dbf14e397d55302022-12-22T01:18:15ZengPress of International Journal of Ophthalmology (IJO PRESS)International Journal of Ophthalmology2222-39592227-48982020-01-011311610.18240/ijo.2020.01.01Changes in corneal innervation and pain responses in fungal keratitisChang-Jie Ren0Yi-Fan Zhou1Yuan Wu2Xu-Dong Peng3Cui Li4Qian Wang5Guo-Qiang Zhu6Jia You7Jie Zhang8Gui-Qiu Zhao9Jing Lin10Department of Ophthalmology, the Affiliated Hospital of Qingdao University, Qingdao 26600, Shandong Province, ChinaDepartment of Ophthalmology, the Affiliated Hospital of Qingdao University, Qingdao 26600, Shandong Province, ChinaDepartment of Ophthalmology, the Affiliated Hospital of Qingdao University, Qingdao 26600, Shandong Province, ChinaDepartment of Ophthalmology, the Affiliated Hospital of Qingdao University, Qingdao 26600, Shandong Province, ChinaDepartment of Ophthalmology, the Affiliated Hospital of Qingdao University, Qingdao 26600, Shandong Province, ChinaDepartment of Ophthalmology, the Affiliated Hospital of Qingdao University, Qingdao 26600, Shandong Province, ChinaDepartment of Ophthalmology, the Affiliated Hospital of Qingdao University, Qingdao 26600, Shandong Province, ChinaDepartment of Ophthalmology, the Affiliated Hospital of Qingdao University, Qingdao 26600, Shandong Province, ChinaDepartment of Ophthalmology, the Affiliated Hospital of Qingdao University, Qingdao 26600, Shandong Province, ChinaDepartment of Ophthalmology, the Affiliated Hospital of Qingdao University, Qingdao 26600, Shandong Province, ChinaDepartment of Ophthalmology, the Affiliated Hospital of Qingdao University, Qingdao 26600, Shandong Province, ChinaAIM: To characterize changes in the cornea nerve and pain responses in fungal keratitis (FK). METHODS: A retrospective analysis of in vivo confocal microscopy images of 11 FK corneas was performed, and the results were compared with those for 11 normal corneas. Subbasal corneal nerves were analyzed for total nerve number, main nerve trunk number, branching patterns and tortuosity. C57BL/6 mice were infected with Aspergillus fumigatus. Disease severity was determined through clinical scoring and slit lamp photography. Corneas were harvested at 1, 3, 5, and 7d post infection (p.i.) and assessed for β III tubulin. Corneal mechanical sensitivity thresholds were detected by von Frey test. β-endorphin (β-EP) and μ receptor protein expression was detected through Western blotting. RESULTS: Total nerve number, main nerve trunk number, and nerve branching were significantly lower in FK patients than in controls, but tortuosity was not significantly different. In infected mice, subbasal nerve density decreased from 1d p.i., reaching a minimum at 5d p.i. Clinical scores rose at 1d p.i., peaked at 3d p.i., and decreased at 5d p.i. Mechanical sensitivity thresholds showed the same trends. β-EP and μ receptor protein expression increased after infection. CONCLUSION: Corneal nerve density is lower in FK patients and Aspergillus fumigatus-infected mice than in controls. Pain sensitivity decreases with postinfection corneal ulcer aggravation. β-EP and μ receptor proteins are both upregulated in infected mouse corneas.http://www.ijo.cn/en_publish/2020/1/20200101.pdfkeratitispainfungalinnervationsubbasal nervemice |
| spellingShingle | Chang-Jie Ren Yi-Fan Zhou Yuan Wu Xu-Dong Peng Cui Li Qian Wang Guo-Qiang Zhu Jia You Jie Zhang Gui-Qiu Zhao Jing Lin Changes in corneal innervation and pain responses in fungal keratitis International Journal of Ophthalmology keratitis pain fungal innervation subbasal nerve mice |
| title | Changes in corneal innervation and pain responses in fungal keratitis |
| title_full | Changes in corneal innervation and pain responses in fungal keratitis |
| title_fullStr | Changes in corneal innervation and pain responses in fungal keratitis |
| title_full_unstemmed | Changes in corneal innervation and pain responses in fungal keratitis |
| title_short | Changes in corneal innervation and pain responses in fungal keratitis |
| title_sort | changes in corneal innervation and pain responses in fungal keratitis |
| topic | keratitis pain fungal innervation subbasal nerve mice |
| url | http://www.ijo.cn/en_publish/2020/1/20200101.pdf |
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