Rice Bran Stabilization Using Autoclave and Optimization of Crude Rice Bran Oil Recovery Using Ultrasound-Assisted Extraction

This research aimed to stabilize rice bran to reduce enzymatic reactions and optimize rice bran oil extraction using ultrasound-assisted extraction (UAE). The optimization was performed using response surface methodology (RSM). The results showed that rice bran oil has three primary fatty acids (ole...

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Main Authors: Dodyk Pranowo, Tsamara Dhany Savira, Sukardi
Format: Article
Language:English
Published: North Carolina State University 2023-10-01
Series:BioResources
Subjects:
Online Access:https://ojs.cnr.ncsu.edu/index.php/BRJ/article/view/22824
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author Dodyk Pranowo
Tsamara Dhany Savira
Sukardi
author_facet Dodyk Pranowo
Tsamara Dhany Savira
Sukardi
author_sort Dodyk Pranowo
collection DOAJ
description This research aimed to stabilize rice bran to reduce enzymatic reactions and optimize rice bran oil extraction using ultrasound-assisted extraction (UAE). The optimization was performed using response surface methodology (RSM). The results showed that rice bran oil has three primary fatty acids (oleic, linoleic, palmitic). Stabilization of rice bran using an autoclave (121 °C) for 10 min was recommended, with oil yield (14.2%), free fatty acid/FFA (0.41%), and gamma oryzanol (16.1 ppm). The storage significantly affected oil yield and gamma oryzanol but not FFA (p>0.05). The untreated and stabilized rice bran FFA during 18-day storage ranged from 1.33 to 5.73% and 0.41 to 1.12%. This confirmed that stabilization could inactivate lipase enzymes by reducing FFA. The optimization showed that the linear model best explained oil yield, total phenol content (TPC), and antioxidant activity (IC50), while the 2FI model was best fitted for gamma oryzanol. The optimal condition was found for UAE extraction at an amplitude of 90% and a time of 30 min. Verifying optimal conditions resulted in oil yield (13.6%), TPC (44.8 mg GAE/g), IC50 (207.2 ppm), and gamma oryzanol (15.8 ppm). Further in-depth studies are required to investigate using green solvents in the UAE and gamma oryzanol purification.
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spelling doaj.art-bc65a0e2fa384a4e83d743cd7d568f722023-11-01T17:13:51ZengNorth Carolina State UniversityBioResources1930-21262023-10-0118483418361890Rice Bran Stabilization Using Autoclave and Optimization of Crude Rice Bran Oil Recovery Using Ultrasound-Assisted ExtractionDodyk Pranowo0Tsamara Dhany Savira1Sukardi2Department of Agro-industrial Technology, Faculty of Agricultural Technology, Universitas Brawijaya, Malang 65145, IndonesiaDepartment of Agro-industrial Technology, Faculty of Agricultural Technology, Universitas Brawijaya, Malang 65145, IndonesiaDepartment of Agro-industrial Technology, Faculty of Agricultural Technology, Universitas Brawijaya, Malang 65145, IndonesiaThis research aimed to stabilize rice bran to reduce enzymatic reactions and optimize rice bran oil extraction using ultrasound-assisted extraction (UAE). The optimization was performed using response surface methodology (RSM). The results showed that rice bran oil has three primary fatty acids (oleic, linoleic, palmitic). Stabilization of rice bran using an autoclave (121 °C) for 10 min was recommended, with oil yield (14.2%), free fatty acid/FFA (0.41%), and gamma oryzanol (16.1 ppm). The storage significantly affected oil yield and gamma oryzanol but not FFA (p>0.05). The untreated and stabilized rice bran FFA during 18-day storage ranged from 1.33 to 5.73% and 0.41 to 1.12%. This confirmed that stabilization could inactivate lipase enzymes by reducing FFA. The optimization showed that the linear model best explained oil yield, total phenol content (TPC), and antioxidant activity (IC50), while the 2FI model was best fitted for gamma oryzanol. The optimal condition was found for UAE extraction at an amplitude of 90% and a time of 30 min. Verifying optimal conditions resulted in oil yield (13.6%), TPC (44.8 mg GAE/g), IC50 (207.2 ppm), and gamma oryzanol (15.8 ppm). Further in-depth studies are required to investigate using green solvents in the UAE and gamma oryzanol purification.https://ojs.cnr.ncsu.edu/index.php/BRJ/article/view/22824autoclaveoptimizationrice bran oilultrasound-assisted extraction
spellingShingle Dodyk Pranowo
Tsamara Dhany Savira
Sukardi
Rice Bran Stabilization Using Autoclave and Optimization of Crude Rice Bran Oil Recovery Using Ultrasound-Assisted Extraction
BioResources
autoclave
optimization
rice bran oil
ultrasound-assisted extraction
title Rice Bran Stabilization Using Autoclave and Optimization of Crude Rice Bran Oil Recovery Using Ultrasound-Assisted Extraction
title_full Rice Bran Stabilization Using Autoclave and Optimization of Crude Rice Bran Oil Recovery Using Ultrasound-Assisted Extraction
title_fullStr Rice Bran Stabilization Using Autoclave and Optimization of Crude Rice Bran Oil Recovery Using Ultrasound-Assisted Extraction
title_full_unstemmed Rice Bran Stabilization Using Autoclave and Optimization of Crude Rice Bran Oil Recovery Using Ultrasound-Assisted Extraction
title_short Rice Bran Stabilization Using Autoclave and Optimization of Crude Rice Bran Oil Recovery Using Ultrasound-Assisted Extraction
title_sort rice bran stabilization using autoclave and optimization of crude rice bran oil recovery using ultrasound assisted extraction
topic autoclave
optimization
rice bran oil
ultrasound-assisted extraction
url https://ojs.cnr.ncsu.edu/index.php/BRJ/article/view/22824
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AT sukardi ricebranstabilizationusingautoclaveandoptimizationofcrudericebranoilrecoveryusingultrasoundassistedextraction