Analysis of efficiency of purification of HEX-oligonucleotides obtained by modified deblocking
A new way of deblocking of synthetic hexachlorofluorescein-oligodeoxyribonucleotides (HEX-ODN) prevents the modification of the label and the accumulation of difficult to separated by-product. It was shown that this method increases the efficiency of purification HEX-ON and the sensitivity of DNA di...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | Russian |
| Published: |
MIREA - Russian Technological University
2012-08-01
|
| Series: | Тонкие химические технологии |
| Subjects: | |
| Online Access: | https://www.finechem-mirea.ru/jour/article/view/719 |
| _version_ | 1797882134325624832 |
|---|---|
| author | A. G. Mosina A. N. Chuvilin I. P. Smirnov V. F. Travkin G. E. Pozmogova |
| author_facet | A. G. Mosina A. N. Chuvilin I. P. Smirnov V. F. Travkin G. E. Pozmogova |
| author_sort | A. G. Mosina |
| collection | DOAJ |
| description | A new way of deblocking of synthetic hexachlorofluorescein-oligodeoxyribonucleotides (HEX-ODN) prevents the modification of the label and the accumulation of difficult to separated by-product. It was shown that this method increases the efficiency of purification HEX-ON and the sensitivity of DNA diagnostics. |
| first_indexed | 2024-04-10T03:31:12Z |
| format | Article |
| id | doaj.art-bc6e658f2afb4a508e4faf4c5933a269 |
| institution | Directory Open Access Journal |
| issn | 2410-6593 2686-7575 |
| language | Russian |
| last_indexed | 2024-04-10T03:31:12Z |
| publishDate | 2012-08-01 |
| publisher | MIREA - Russian Technological University |
| record_format | Article |
| series | Тонкие химические технологии |
| spelling | doaj.art-bc6e658f2afb4a508e4faf4c5933a2692023-03-13T07:25:33ZrusMIREA - Russian Technological UniversityТонкие химические технологии2410-65932686-75752012-08-01747274713Analysis of efficiency of purification of HEX-oligonucleotides obtained by modified deblockingA. G. Mosina0A. N. Chuvilin1I. P. Smirnov2V. F. Travkin3G. E. Pozmogova4M.V. Lomonosov Moscow State University of Fine Chemical Technologies, 86, Vernadskogo pr., Moscow 119571Научно-исследовательский институт физико-химической медицины Федерального медико-биологического агентства РоссииНаучно-исследовательский институт физико-химической медицины Федерального медико-биологического агентства РоссииM.V. Lomonosov Moscow State University of Fine Chemical Technologies, 86, Vernadskogo pr., Moscow 119571МИТХТ им. М.В. ЛомоносоваA new way of deblocking of synthetic hexachlorofluorescein-oligodeoxyribonucleotides (HEX-ODN) prevents the modification of the label and the accumulation of difficult to separated by-product. It was shown that this method increases the efficiency of purification HEX-ON and the sensitivity of DNA diagnostics.https://www.finechem-mirea.ru/jour/article/view/719hexachlorofluorescein lаbeloligodeoxyribonucleotideshplcreal time pcr |
| spellingShingle | A. G. Mosina A. N. Chuvilin I. P. Smirnov V. F. Travkin G. E. Pozmogova Analysis of efficiency of purification of HEX-oligonucleotides obtained by modified deblocking Тонкие химические технологии hexachlorofluorescein lаbel oligodeoxyribonucleotides hplc real time pcr |
| title | Analysis of efficiency of purification of HEX-oligonucleotides obtained by modified deblocking |
| title_full | Analysis of efficiency of purification of HEX-oligonucleotides obtained by modified deblocking |
| title_fullStr | Analysis of efficiency of purification of HEX-oligonucleotides obtained by modified deblocking |
| title_full_unstemmed | Analysis of efficiency of purification of HEX-oligonucleotides obtained by modified deblocking |
| title_short | Analysis of efficiency of purification of HEX-oligonucleotides obtained by modified deblocking |
| title_sort | analysis of efficiency of purification of hex oligonucleotides obtained by modified deblocking |
| topic | hexachlorofluorescein lаbel oligodeoxyribonucleotides hplc real time pcr |
| url | https://www.finechem-mirea.ru/jour/article/view/719 |
| work_keys_str_mv | AT agmosina analysisofefficiencyofpurificationofhexoligonucleotidesobtainedbymodifieddeblocking AT anchuvilin analysisofefficiencyofpurificationofhexoligonucleotidesobtainedbymodifieddeblocking AT ipsmirnov analysisofefficiencyofpurificationofhexoligonucleotidesobtainedbymodifieddeblocking AT vftravkin analysisofefficiencyofpurificationofhexoligonucleotidesobtainedbymodifieddeblocking AT gepozmogova analysisofefficiencyofpurificationofhexoligonucleotidesobtainedbymodifieddeblocking |