In Vitro Antibacterial Activity of Ethanolic Extract of Aloe Vera and Silver Nanoparticles on Standard Strains of Some Pathogenic Bacteria
Background and objectives: Today, with the increasing rate of antibiotic resistance, treatment of bacterial infections has become challenging. Therefore, it is essential to find suitable alternative antibacterial compounds. The aim of this study was to investigate in vitro effects of silver nanopart...
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| Format: | Article |
| Language: | English |
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Golestan University Of Medical Sciences
2021-12-01
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| Series: | Journal of Clinical and Basic Research |
| Subjects: | |
| Online Access: | http://jcbr.goums.ac.ir/article-1-332-en.html |
| _version_ | 1797974514831720448 |
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| author | Abolfazl jafari-Sales Parisa Meshinchi Ali Shabkhosh |
| author_facet | Abolfazl jafari-Sales Parisa Meshinchi Ali Shabkhosh |
| author_sort | Abolfazl jafari-Sales |
| collection | DOAJ |
| description | Background and objectives: Today, with the increasing rate of antibiotic resistance, treatment of bacterial infections has become challenging. Therefore, it is essential to find suitable alternative antibacterial compounds. The aim of this study was to investigate in vitro effects of silver nanoparticles and ethanolic extract of Aloe vera alone and combined on standard strains of some pathogenic bacteria.
Methods: After collection and verification of A. vera plants, extraction was performed by the Soxhlet extractor method. Antibacterial effects of ethanolic extract of A. vera and silver nanoparticles on standard strains of Staphylococcus aureus, Bacillus cereus, Escherichia coli and Pseudomonas aeruginosa were investigated by agar well diffusion and tube dilution methods, respectively.
Results: The ethanolic extract of A. vera and silver nanoparticles had antibacterial effects on the tested bacteria in a dose-dependent manner. The ethanolic extract of A. vera was more effective against S. aureus and B. cereus compared to gram-negative bacteria. However, silver nanoparticles were more effective against gram-negative bacteria (P. aeruginosa and E. coli). The effect of combination of ethanolic extract of A. vera and silver nanoparticles was much greater than the effect of either alone. This combination showed the greatest and lowest effect on P. aeruginosa and S. aureus, respectively.
Conclusion: For the first time, this study showed that the combination of ethanolic extract of A. vera and silver nanoparticles is effective against potentially pathogenic bacteria. Given the high rate of antibiotic resistance and side effects of conventional antibiotics, it is recommended to identify active compounds of this plant and evaluate the antimicrobial effects of this combination of fungi and other pathogenic bacteria both in vitro and in vivo. |
| first_indexed | 2024-04-11T04:20:59Z |
| format | Article |
| id | doaj.art-bc71846ac6234be1a9741f4767a7b4a6 |
| institution | Directory Open Access Journal |
| issn | 2538-3736 |
| language | English |
| last_indexed | 2024-04-11T04:20:59Z |
| publishDate | 2021-12-01 |
| publisher | Golestan University Of Medical Sciences |
| record_format | Article |
| series | Journal of Clinical and Basic Research |
| spelling | doaj.art-bc71846ac6234be1a9741f4767a7b4a62022-12-30T17:26:03ZengGolestan University Of Medical SciencesJournal of Clinical and Basic Research2538-37362021-12-01542230In Vitro Antibacterial Activity of Ethanolic Extract of Aloe Vera and Silver Nanoparticles on Standard Strains of Some Pathogenic BacteriaAbolfazl jafari-Sales0Parisa Meshinchi1Ali Shabkhosh2 Department of Microbiology School of Basic Sciences, Kazerun Branch, Islamic Azad University, Kazerun, Iran Department of Chemical Engineering, University of Tabriz, Tabriz, Iran Department of Microbiology School of Basic Sciences, Ahar Branch, Islamic Azad University, Ahar, Iran Background and objectives: Today, with the increasing rate of antibiotic resistance, treatment of bacterial infections has become challenging. Therefore, it is essential to find suitable alternative antibacterial compounds. The aim of this study was to investigate in vitro effects of silver nanoparticles and ethanolic extract of Aloe vera alone and combined on standard strains of some pathogenic bacteria. Methods: After collection and verification of A. vera plants, extraction was performed by the Soxhlet extractor method. Antibacterial effects of ethanolic extract of A. vera and silver nanoparticles on standard strains of Staphylococcus aureus, Bacillus cereus, Escherichia coli and Pseudomonas aeruginosa were investigated by agar well diffusion and tube dilution methods, respectively. Results: The ethanolic extract of A. vera and silver nanoparticles had antibacterial effects on the tested bacteria in a dose-dependent manner. The ethanolic extract of A. vera was more effective against S. aureus and B. cereus compared to gram-negative bacteria. However, silver nanoparticles were more effective against gram-negative bacteria (P. aeruginosa and E. coli). The effect of combination of ethanolic extract of A. vera and silver nanoparticles was much greater than the effect of either alone. This combination showed the greatest and lowest effect on P. aeruginosa and S. aureus, respectively. Conclusion: For the first time, this study showed that the combination of ethanolic extract of A. vera and silver nanoparticles is effective against potentially pathogenic bacteria. Given the high rate of antibiotic resistance and side effects of conventional antibiotics, it is recommended to identify active compounds of this plant and evaluate the antimicrobial effects of this combination of fungi and other pathogenic bacteria both in vitro and in vivo.http://jcbr.goums.ac.ir/article-1-332-en.htmlantibacterial susceptibilityaloe verananoparticlessilverplant extract |
| spellingShingle | Abolfazl jafari-Sales Parisa Meshinchi Ali Shabkhosh In Vitro Antibacterial Activity of Ethanolic Extract of Aloe Vera and Silver Nanoparticles on Standard Strains of Some Pathogenic Bacteria Journal of Clinical and Basic Research antibacterial susceptibility aloe vera nanoparticles silver plant extract |
| title | In Vitro Antibacterial Activity of Ethanolic Extract of Aloe Vera and Silver Nanoparticles on Standard Strains of Some Pathogenic Bacteria |
| title_full | In Vitro Antibacterial Activity of Ethanolic Extract of Aloe Vera and Silver Nanoparticles on Standard Strains of Some Pathogenic Bacteria |
| title_fullStr | In Vitro Antibacterial Activity of Ethanolic Extract of Aloe Vera and Silver Nanoparticles on Standard Strains of Some Pathogenic Bacteria |
| title_full_unstemmed | In Vitro Antibacterial Activity of Ethanolic Extract of Aloe Vera and Silver Nanoparticles on Standard Strains of Some Pathogenic Bacteria |
| title_short | In Vitro Antibacterial Activity of Ethanolic Extract of Aloe Vera and Silver Nanoparticles on Standard Strains of Some Pathogenic Bacteria |
| title_sort | in vitro antibacterial activity of ethanolic extract of aloe vera and silver nanoparticles on standard strains of some pathogenic bacteria |
| topic | antibacterial susceptibility aloe vera nanoparticles silver plant extract |
| url | http://jcbr.goums.ac.ir/article-1-332-en.html |
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