Head-to-Head Comparison of Different Blood Collecting Tubes for Quantification of Alzheimer’s Disease Biomarkers in Plasma
To examine whether the type of blood collection tubes affects the quantification of plasma biomarkers for Alzheimer’s disease analyzed with a single-molecule array (Simoa), we recruited a healthy cohort (n = 34, 11 males, mean age = 28.7 ± 7.55) and collected plasma in the following tubes: dipotassi...
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| Format: | Article |
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MDPI AG
2022-08-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/12/9/1194 |
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| author | Lijun Jiang Xulong Ding Wenxiao Wang Xiaobin Yang Tao Li Peng Lei |
| author_facet | Lijun Jiang Xulong Ding Wenxiao Wang Xiaobin Yang Tao Li Peng Lei |
| author_sort | Lijun Jiang |
| collection | DOAJ |
| description | To examine whether the type of blood collection tubes affects the quantification of plasma biomarkers for Alzheimer’s disease analyzed with a single-molecule array (Simoa), we recruited a healthy cohort (n = 34, 11 males, mean age = 28.7 ± 7.55) and collected plasma in the following tubes: dipotassium ethylenediaminetetraacetic acid (K2-EDTA), heparin lithium (Li-Hep), and heparin sodium (Na-Hep). Plasma tau, phosphorylated tau 181 (p-tau181), amyloid β (1–40) (Aβ40), and amyloid β (1–42) (Aβ42) were quantified using Simoa. We compared the value of plasma analytes, as well as the effects of sex on the measurements. We found that plasma collected in Li-Hep and Na-Hep tubes yielded significantly higher tau and p-tau181 levels compared to plasma collected in K2-EDTA tubes from the same person, but there was no difference in the measured values of the Aβ40, Aβ42, and Aβ42/40 ratio. Therefore, the type of blood collecting tubes should be considered when planning studies that measure plasma tau. |
| first_indexed | 2024-03-10T00:37:34Z |
| format | Article |
| id | doaj.art-bc731a9e828e4cff901cffa544b90f4b |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-10T00:37:34Z |
| publishDate | 2022-08-01 |
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| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-bc731a9e828e4cff901cffa544b90f4b2023-11-23T15:14:27ZengMDPI AGBiomolecules2218-273X2022-08-01129119410.3390/biom12091194Head-to-Head Comparison of Different Blood Collecting Tubes for Quantification of Alzheimer’s Disease Biomarkers in PlasmaLijun Jiang0Xulong Ding1Wenxiao Wang2Xiaobin Yang3Tao Li4Peng Lei5Mental Health Center and Department of Neurology, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, ChinaMental Health Center and Department of Neurology, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, ChinaDeyang Mental Health Center, Deyang 618099, ChinaDeyang Mental Health Center, Deyang 618099, ChinaMental Health Center and Department of Neurology, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, ChinaMental Health Center and Department of Neurology, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, ChinaTo examine whether the type of blood collection tubes affects the quantification of plasma biomarkers for Alzheimer’s disease analyzed with a single-molecule array (Simoa), we recruited a healthy cohort (n = 34, 11 males, mean age = 28.7 ± 7.55) and collected plasma in the following tubes: dipotassium ethylenediaminetetraacetic acid (K2-EDTA), heparin lithium (Li-Hep), and heparin sodium (Na-Hep). Plasma tau, phosphorylated tau 181 (p-tau181), amyloid β (1–40) (Aβ40), and amyloid β (1–42) (Aβ42) were quantified using Simoa. We compared the value of plasma analytes, as well as the effects of sex on the measurements. We found that plasma collected in Li-Hep and Na-Hep tubes yielded significantly higher tau and p-tau181 levels compared to plasma collected in K2-EDTA tubes from the same person, but there was no difference in the measured values of the Aβ40, Aβ42, and Aβ42/40 ratio. Therefore, the type of blood collecting tubes should be considered when planning studies that measure plasma tau.https://www.mdpi.com/2218-273X/12/9/1194blood collection tubestauAlzheimer’s diseaseSimoaEDTAheparin |
| spellingShingle | Lijun Jiang Xulong Ding Wenxiao Wang Xiaobin Yang Tao Li Peng Lei Head-to-Head Comparison of Different Blood Collecting Tubes for Quantification of Alzheimer’s Disease Biomarkers in Plasma Biomolecules blood collection tubes tau Alzheimer’s disease Simoa EDTA heparin |
| title | Head-to-Head Comparison of Different Blood Collecting Tubes for Quantification of Alzheimer’s Disease Biomarkers in Plasma |
| title_full | Head-to-Head Comparison of Different Blood Collecting Tubes for Quantification of Alzheimer’s Disease Biomarkers in Plasma |
| title_fullStr | Head-to-Head Comparison of Different Blood Collecting Tubes for Quantification of Alzheimer’s Disease Biomarkers in Plasma |
| title_full_unstemmed | Head-to-Head Comparison of Different Blood Collecting Tubes for Quantification of Alzheimer’s Disease Biomarkers in Plasma |
| title_short | Head-to-Head Comparison of Different Blood Collecting Tubes for Quantification of Alzheimer’s Disease Biomarkers in Plasma |
| title_sort | head to head comparison of different blood collecting tubes for quantification of alzheimer s disease biomarkers in plasma |
| topic | blood collection tubes tau Alzheimer’s disease Simoa EDTA heparin |
| url | https://www.mdpi.com/2218-273X/12/9/1194 |
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