Longitudinal study of reproductive performance of female cattle produced by somatic cell nuclear transfer.
The objective of this study was to determine whether or not reproductive performance in cattle produced by somatic cell nuclear transfer (SCNT) is significantly different from that of their genetic donors. To address this question, we directed two longitudinal studies using different embryo producti...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3877258?pdf=render |
| _version_ | 1818203973564235776 |
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| author | Irina A Polejaeva Diane M Broek Shawn C Walker Wenli Zhou Mark Walton Abby D Benninghoff David C Faber |
| author_facet | Irina A Polejaeva Diane M Broek Shawn C Walker Wenli Zhou Mark Walton Abby D Benninghoff David C Faber |
| author_sort | Irina A Polejaeva |
| collection | DOAJ |
| description | The objective of this study was to determine whether or not reproductive performance in cattle produced by somatic cell nuclear transfer (SCNT) is significantly different from that of their genetic donors. To address this question, we directed two longitudinal studies using different embryo production procedures: (1) superovulation followed by artificial insemination (AI) and embryo collection and (2) ultrasound-guided ovum pick-up followed by in vitro fertilization (OPU-IVF). Collectively, these two studies represent the largest data set available for any species on the reproductive performance of female clones and their genetic donors as measured by their embryo production outcomes in commercial embryo production program. The large-scale study described herein was conducted over a six-year period of time and provides a unique comparison of 96 clones to the 40 corresponding genetic donors. To our knowledge, this is the first longitudinal study on the reproductive performance of cattle clones using OPU-IVF. With nearly 2,000 reproductive procedures performed and more than 9,200 transferable embryos produced, our observations show that the reproductive performance of cattle produced by SCNT is not different compared to their genetic donors for the production of transferable embryos after either AI followed by embryo collection (P = 0.77) or OPU-IVF (P = 0.97). These data are in agreement with previous reports showing that the reproductive capabilities of cloned cattle are equal to that of conventionally produced cattle. In conclusion, results of this longitudinal study once again demonstrate that cloning technology, in combination with superovulation, AI and embryo collection or OPU-IVF, provides a valuable tool for faster dissemination of superior maternal genetics. |
| first_indexed | 2024-12-12T03:33:51Z |
| format | Article |
| id | doaj.art-bc73c61ab3ab40e5b4d1d60922e27783 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-12T03:33:51Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-bc73c61ab3ab40e5b4d1d60922e277832022-12-22T00:39:51ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-01812e8428310.1371/journal.pone.0084283Longitudinal study of reproductive performance of female cattle produced by somatic cell nuclear transfer.Irina A PolejaevaDiane M BroekShawn C WalkerWenli ZhouMark WaltonAbby D BenninghoffDavid C FaberThe objective of this study was to determine whether or not reproductive performance in cattle produced by somatic cell nuclear transfer (SCNT) is significantly different from that of their genetic donors. To address this question, we directed two longitudinal studies using different embryo production procedures: (1) superovulation followed by artificial insemination (AI) and embryo collection and (2) ultrasound-guided ovum pick-up followed by in vitro fertilization (OPU-IVF). Collectively, these two studies represent the largest data set available for any species on the reproductive performance of female clones and their genetic donors as measured by their embryo production outcomes in commercial embryo production program. The large-scale study described herein was conducted over a six-year period of time and provides a unique comparison of 96 clones to the 40 corresponding genetic donors. To our knowledge, this is the first longitudinal study on the reproductive performance of cattle clones using OPU-IVF. With nearly 2,000 reproductive procedures performed and more than 9,200 transferable embryos produced, our observations show that the reproductive performance of cattle produced by SCNT is not different compared to their genetic donors for the production of transferable embryos after either AI followed by embryo collection (P = 0.77) or OPU-IVF (P = 0.97). These data are in agreement with previous reports showing that the reproductive capabilities of cloned cattle are equal to that of conventionally produced cattle. In conclusion, results of this longitudinal study once again demonstrate that cloning technology, in combination with superovulation, AI and embryo collection or OPU-IVF, provides a valuable tool for faster dissemination of superior maternal genetics.http://europepmc.org/articles/PMC3877258?pdf=render |
| spellingShingle | Irina A Polejaeva Diane M Broek Shawn C Walker Wenli Zhou Mark Walton Abby D Benninghoff David C Faber Longitudinal study of reproductive performance of female cattle produced by somatic cell nuclear transfer. PLoS ONE |
| title | Longitudinal study of reproductive performance of female cattle produced by somatic cell nuclear transfer. |
| title_full | Longitudinal study of reproductive performance of female cattle produced by somatic cell nuclear transfer. |
| title_fullStr | Longitudinal study of reproductive performance of female cattle produced by somatic cell nuclear transfer. |
| title_full_unstemmed | Longitudinal study of reproductive performance of female cattle produced by somatic cell nuclear transfer. |
| title_short | Longitudinal study of reproductive performance of female cattle produced by somatic cell nuclear transfer. |
| title_sort | longitudinal study of reproductive performance of female cattle produced by somatic cell nuclear transfer |
| url | http://europepmc.org/articles/PMC3877258?pdf=render |
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