Screening of Various Organic Substrates and the Development of a Suitable Low-Cost Fermentation Medium for the Production of α-Amylase by Bacillus subtilis
The production of extracellular amylase by Bacillus subtilis has been studied in solid-state fermentation (SSF). In a sequential order, various process parameters were optimized for maximum amylase production. The tested process parameters were different solid substrates such as banana husk (BH), wa...
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| Format: | Article |
| Language: | English |
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University of Zagreb Faculty of Food Technology and Biotechnology
2009-01-01
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| Series: | Food Technology and Biotechnology |
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| Online Access: | http://hrcak.srce.hr/file/68378 |
| _version_ | 1797423094102491136 |
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| author | Sadin Özdemir Zübeyde Baysal Fikret Uyar Kemal Güven |
| author_facet | Sadin Özdemir Zübeyde Baysal Fikret Uyar Kemal Güven |
| author_sort | Sadin Özdemir |
| collection | DOAJ |
| description | The production of extracellular amylase by Bacillus subtilis has been studied in solid-state fermentation (SSF). In a sequential order, various process parameters were optimized for maximum amylase production. The tested process parameters were different solid substrates such as banana husk (BH), water melon husk (WMH), lentil bran (LB), wheat bran (WB), melon husk (MH) and maize oil cake (MOC), different incubation time (24–144 h), particle size (500–2500 μm), initial moisture content of the substrate (40–70 %, by mass per volume), inoculum size (10–60 %, by mass per volume) and inoculum concentration (10–60 %, by volume per mass). The maximum production of amylase (4857 U/mg) was achieved when 1 % starch (by mass, particle size of 1500 μm) was added to banana husk as the solid substrate, fermented for 72 h at 37 °C, at an inoculum level of 30 % (by volume per mass), and initial moisture content of 60 % (by volume per mass). |
| first_indexed | 2024-03-09T07:42:30Z |
| format | Article |
| id | doaj.art-bc76c590fc454e5d95a6bc4cbc1820a9 |
| institution | Directory Open Access Journal |
| issn | 1330-9862 1334-2606 |
| language | English |
| last_indexed | 2024-03-09T07:42:30Z |
| publishDate | 2009-01-01 |
| publisher | University of Zagreb Faculty of Food Technology and Biotechnology |
| record_format | Article |
| series | Food Technology and Biotechnology |
| spelling | doaj.art-bc76c590fc454e5d95a6bc4cbc1820a92023-12-03T04:22:01ZengUniversity of Zagreb Faculty of Food Technology and BiotechnologyFood Technology and Biotechnology1330-98621334-26062009-01-01474364369Screening of Various Organic Substrates and the Development of a Suitable Low-Cost Fermentation Medium for the Production of α-Amylase by Bacillus subtilisSadin Özdemir0Zübeyde Baysal1Fikret Uyar2Kemal Güven3Molecular Biology and Genetics Section, Science Faculty, Dicle University, TR-21280 Diyarbakir, TurkeyChemistry Department, Science Faculty, Dicle University, TR-21280 Diyarbakir, TurkeyMolecular Biology and Genetics Section, Science Faculty, Dicle University, TR-21280 Diyarbakir, TurkeyMolecular Biology and Genetics Section, Science Faculty, Dicle University, TR-21280 Diyarbakir, TurkeyThe production of extracellular amylase by Bacillus subtilis has been studied in solid-state fermentation (SSF). In a sequential order, various process parameters were optimized for maximum amylase production. The tested process parameters were different solid substrates such as banana husk (BH), water melon husk (WMH), lentil bran (LB), wheat bran (WB), melon husk (MH) and maize oil cake (MOC), different incubation time (24–144 h), particle size (500–2500 μm), initial moisture content of the substrate (40–70 %, by mass per volume), inoculum size (10–60 %, by mass per volume) and inoculum concentration (10–60 %, by volume per mass). The maximum production of amylase (4857 U/mg) was achieved when 1 % starch (by mass, particle size of 1500 μm) was added to banana husk as the solid substrate, fermented for 72 h at 37 °C, at an inoculum level of 30 % (by volume per mass), and initial moisture content of 60 % (by volume per mass).http://hrcak.srce.hr/file/68378Bacillus subtilisα-amylasebanana husksolid-state fermentation |
| spellingShingle | Sadin Özdemir Zübeyde Baysal Fikret Uyar Kemal Güven Screening of Various Organic Substrates and the Development of a Suitable Low-Cost Fermentation Medium for the Production of α-Amylase by Bacillus subtilis Food Technology and Biotechnology Bacillus subtilis α-amylase banana husk solid-state fermentation |
| title | Screening of Various Organic Substrates and the Development of a Suitable Low-Cost Fermentation Medium for the Production of α-Amylase by Bacillus subtilis |
| title_full | Screening of Various Organic Substrates and the Development of a Suitable Low-Cost Fermentation Medium for the Production of α-Amylase by Bacillus subtilis |
| title_fullStr | Screening of Various Organic Substrates and the Development of a Suitable Low-Cost Fermentation Medium for the Production of α-Amylase by Bacillus subtilis |
| title_full_unstemmed | Screening of Various Organic Substrates and the Development of a Suitable Low-Cost Fermentation Medium for the Production of α-Amylase by Bacillus subtilis |
| title_short | Screening of Various Organic Substrates and the Development of a Suitable Low-Cost Fermentation Medium for the Production of α-Amylase by Bacillus subtilis |
| title_sort | screening of various organic substrates and the development of a suitable low cost fermentation medium for the production of α amylase by bacillus subtilis |
| topic | Bacillus subtilis α-amylase banana husk solid-state fermentation |
| url | http://hrcak.srce.hr/file/68378 |
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