Microalgae-based drug delivery system for tumor microenvironment photo-modulating and synergistic chemo-photodynamic therapy of osteosarcoma
Osteosarcoma (OS) is one of the most common malignant tumors in children and young adults. As chemotherapy and other therapies are limited by low therapeutic efficiency, severe side effects and single therapeutic function, it is of high value to develop innovative therapies for precise and efficient...
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| Format: | Article |
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KeAi Communications Co., Ltd.
2024-06-01
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| Series: | Engineered Regeneration |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666138124000161 |
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| author | Feng Liang Xueying An Ruoxi Wang Wenshu Wu Lin Yang Yixin Zheng Qing Jiang Xingquan Xu Danni Zhong Min Zhou |
| author_facet | Feng Liang Xueying An Ruoxi Wang Wenshu Wu Lin Yang Yixin Zheng Qing Jiang Xingquan Xu Danni Zhong Min Zhou |
| author_sort | Feng Liang |
| collection | DOAJ |
| description | Osteosarcoma (OS) is one of the most common malignant tumors in children and young adults. As chemotherapy and other therapies are limited by low therapeutic efficiency, severe side effects and single therapeutic function, it is of high value to develop innovative therapies for precise and efficient treatment of OS. Herein, natural photosynthetic microalgae (C. vulgaris, CV) were utilized as carriers for the chemotherapeutic agent doxorubicin (DOX) to create a multifunctional therapeutic platform (CV@DOX) for the photo-modulation of the tumor microenvironment (TME) and synergistic chemo-photodynamic therapy of osteosarcoma. CV@DOX exhibited rapid drug release behavior in the acidic TME, improving the efficiency of chemotherapy against tumors and reducing side effects on other normal tissues. Under 650 nm laser irradiation, CV@DOX demonstrated the ability to effectively generate oxygen to alleviate tumor hypoxia and utilize the photosensitizing properties of chlorophyll in CV to produce an increased amount of reactive oxygen species (ROS), thereby enhancing photodynamic therapy (PDT). CV@DOX-mediated synergistic chemo-photodynamic therapy demonstrated efficacy in halting tumor progression in an orthotopic osteosarcoma mouse model by promoting tumor cell apoptosis, inhibiting tumor proliferation and angiogenesis. Moreover, chlorophyll-assisted fluorescence imaging enabled monitoring of the distribution of CV@DOX in osteosarcoma after administration. Finally, CV@DOX did not cause significant hematological and tissue toxicity, and prevented DOX-induced cardiotoxicity, showing good in vivo biocompatibility. Overall, this work presents a novel TME-responsive and TME-modulating platform for imaging-guided multimodal osteosarcoma treatment. |
| first_indexed | 2024-04-24T11:37:36Z |
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| id | doaj.art-bc78b4aeb1cf4dbcae67219dc32da80b |
| institution | Directory Open Access Journal |
| issn | 2666-1381 |
| language | English |
| last_indexed | 2025-03-21T16:54:29Z |
| publishDate | 2024-06-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Engineered Regeneration |
| spelling | doaj.art-bc78b4aeb1cf4dbcae67219dc32da80b2024-06-15T06:13:06ZengKeAi Communications Co., Ltd.Engineered Regeneration2666-13812024-06-0152199209Microalgae-based drug delivery system for tumor microenvironment photo-modulating and synergistic chemo-photodynamic therapy of osteosarcomaFeng Liang0Xueying An1Ruoxi Wang2Wenshu Wu3Lin Yang4Yixin Zheng5Qing Jiang6Xingquan Xu7Danni Zhong8Min Zhou9Eye Center, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310000, PR China; Department of Orthopedics, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310000, PR ChinaState Key Laboratory of Pharmaceutical Biotechnology, Division of Sports Medicine and Adult Reconstructive Surgery, Department of Orthopedic Surgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, 210008, PR ChinaInstitute of Translational Medicine, Zhejiang University, Hangzhou, 310029, PR ChinaState Key Laboratory of Pharmaceutical Biotechnology, Division of Sports Medicine and Adult Reconstructive Surgery, Department of Orthopedic Surgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, 210008, PR ChinaState Key Laboratory of Pharmaceutical Biotechnology, Division of Sports Medicine and Adult Reconstructive Surgery, Department of Orthopedic Surgery, Nanjing Drum Tower Hospital, Clinical College of Nanjing Medical University, Nanjing, 210008, PR ChinaDepartment of Orthopedics, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310000, PR ChinaState Key Laboratory of Pharmaceutical Biotechnology, Division of Sports Medicine and Adult Reconstructive Surgery, Department of Orthopedic Surgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, 210008, PR China; Corresponding authors.State Key Laboratory of Pharmaceutical Biotechnology, Division of Sports Medicine and Adult Reconstructive Surgery, Department of Orthopedic Surgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, 210008, PR China; Corresponding authors.Institute of Translational Medicine, Zhejiang University, Hangzhou, 310029, PR China; Corresponding authors.Eye Center, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310000, PR China; Zhejiang University-University of Edinburgh Institute (ZJU-UoE Institute), Zhejiang University School of Medicine, Zhejiang University, Haining, 314400, PR China; Zhejiang University-Ordos City Etuoke Banner Joint Research Center, Zhejiang University, Haining, 314400, PR China; Corresponding authors.Osteosarcoma (OS) is one of the most common malignant tumors in children and young adults. As chemotherapy and other therapies are limited by low therapeutic efficiency, severe side effects and single therapeutic function, it is of high value to develop innovative therapies for precise and efficient treatment of OS. Herein, natural photosynthetic microalgae (C. vulgaris, CV) were utilized as carriers for the chemotherapeutic agent doxorubicin (DOX) to create a multifunctional therapeutic platform (CV@DOX) for the photo-modulation of the tumor microenvironment (TME) and synergistic chemo-photodynamic therapy of osteosarcoma. CV@DOX exhibited rapid drug release behavior in the acidic TME, improving the efficiency of chemotherapy against tumors and reducing side effects on other normal tissues. Under 650 nm laser irradiation, CV@DOX demonstrated the ability to effectively generate oxygen to alleviate tumor hypoxia and utilize the photosensitizing properties of chlorophyll in CV to produce an increased amount of reactive oxygen species (ROS), thereby enhancing photodynamic therapy (PDT). CV@DOX-mediated synergistic chemo-photodynamic therapy demonstrated efficacy in halting tumor progression in an orthotopic osteosarcoma mouse model by promoting tumor cell apoptosis, inhibiting tumor proliferation and angiogenesis. Moreover, chlorophyll-assisted fluorescence imaging enabled monitoring of the distribution of CV@DOX in osteosarcoma after administration. Finally, CV@DOX did not cause significant hematological and tissue toxicity, and prevented DOX-induced cardiotoxicity, showing good in vivo biocompatibility. Overall, this work presents a novel TME-responsive and TME-modulating platform for imaging-guided multimodal osteosarcoma treatment.http://www.sciencedirect.com/science/article/pii/S2666138124000161OsteosarcomaTumor hypoxiaPhotodynamicChemotherapySynergistic therapy |
| spellingShingle | Feng Liang Xueying An Ruoxi Wang Wenshu Wu Lin Yang Yixin Zheng Qing Jiang Xingquan Xu Danni Zhong Min Zhou Microalgae-based drug delivery system for tumor microenvironment photo-modulating and synergistic chemo-photodynamic therapy of osteosarcoma Engineered Regeneration Osteosarcoma Tumor hypoxia Photodynamic Chemotherapy Synergistic therapy |
| title | Microalgae-based drug delivery system for tumor microenvironment photo-modulating and synergistic chemo-photodynamic therapy of osteosarcoma |
| title_full | Microalgae-based drug delivery system for tumor microenvironment photo-modulating and synergistic chemo-photodynamic therapy of osteosarcoma |
| title_fullStr | Microalgae-based drug delivery system for tumor microenvironment photo-modulating and synergistic chemo-photodynamic therapy of osteosarcoma |
| title_full_unstemmed | Microalgae-based drug delivery system for tumor microenvironment photo-modulating and synergistic chemo-photodynamic therapy of osteosarcoma |
| title_short | Microalgae-based drug delivery system for tumor microenvironment photo-modulating and synergistic chemo-photodynamic therapy of osteosarcoma |
| title_sort | microalgae based drug delivery system for tumor microenvironment photo modulating and synergistic chemo photodynamic therapy of osteosarcoma |
| topic | Osteosarcoma Tumor hypoxia Photodynamic Chemotherapy Synergistic therapy |
| url | http://www.sciencedirect.com/science/article/pii/S2666138124000161 |
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